AB126269

Anti-UGT2B7 antibody

Name: Anti-UGT2B7 antibody (ab126269) | Abcam
Brand: Abcam Limited
SKU: AB126269
Price: 520 USD
Availability: InStock

Be the first to review this product! Submit a review

(6 Publications)

Rabbit Polyclonal UGT2B7 antibody. Suitable for WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human UGT2B7 aa 150-500.

View Alternative Names

UGTB2B9, UGT2B7, UDP-glucuronosyltransferase 2B7, UDPGT 2B7, UDP-glucuronosyltransferase 2B9, UDPGTh-2, UDPGT 2B9

2 Images

Unknown

Western blot - Anti-UGT2B7 antibody (AB126269)

7.5% SDS PAGE

All lanes:

Western blot - Anti-UGT2B7 antibody (ab126269) at 1/1000 dilution

All lanes:

HepG2 whole cell lysate at 30 µg

Predicted band size: 61 kDa

false

CiteAb

Western blot - Anti-UGT2B7 antibody (AB126269)

UGT2B7 western blot using anti-UGT2B7 antibody ab126269. Publication image and figure legend from Yang, Z., Li, L., et al., 2016, Front Pharmacol, PubMed 27847477.

ab126269 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab126269 please see the product overview.

Alteration Analysis of genes correlated to morphine tolerance after UGT2B7 knocked down in morphine tolerance-like HL-7702 cells. (A) UGT2B7 mRNA expression or (B) protein expression was determined in the cells which transfected with siRNA (UGT2B7). Tolerance related genes of (C) MOR or (D) CaMKIIα mRNA expression was determined in siRNA(UGT2B7) transfection cells pretreated with 50 μM LCA for 48 h then co-incubated with 1 μM morphine for additional 48 h. Results were normalized to GAPDH with mean ± SEM of three independent experiments. DMSO or NC RNA were used as control. Pairwise comparisons were calculated by student t-test to calculate P-values (**p < 0.01, *p < 0.05).

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Recombinant Fragment Protein within Human UGT2B7 aa 150-500. The exact immunogen used to generate this antibody is proprietary information.

P16662

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UGT2B7 also known as UDP-glucuronosyltransferase 2B7 is an enzyme that helps in the process of glucuronidation. This enzyme has a molecular mass of approximately 56 kDa. It is part of the UGT2B family and is mainly expressed in the liver but also found in other tissues like the kidney and intestines. UGT2B7 plays an important role in metabolizing diverse compounds including drugs and endogenous substances by adding glucuronic acid to them making them more water-soluble and easier to excrete.

Biological function summary

The glucuronidation function of UGT2B7 is important for detoxifying and eliminating hormones bilirubin and xenobiotics from the body. This enzyme does not function as part of a complex; instead it acts individually. The activity of UGT2B7 aids in maintaining homeostasis by processing toxins and facilitating their clearance from the bloodstream. As a member of the large family of UDP-glucuronosyltransferases UGT2B7 exhibits substrate specificity which ensures efficient metabolism of various substrates.

Pathways

UGT2B7 contributes to the drug metabolism pathway and bilirubin clearance pathway. It works closely with other enzymes such as cytochrome P450s which modify the substrates before UGT2B7 adds glucuronic acid. In the drug metabolism pathway the enzyme participates directly in the phase II reaction stages converting lipophilic drug molecules into hydrophilic metabolites. The enzyme's role in bilirubin clearance assists in preventing the buildup of this compound which could lead to harmful conditions.

Faulty UGT2B7 activity correlates with conditions like Gilbert's syndrome and adverse drug reactions. Gilbert’s syndrome a mild genetic condition results from reduced glucuronidation capability leading to increased levels of unconjugated bilirubin. The interaction with drugs can also impact their pharmacokinetics and dynamics resulting in toxicity or ineffectiveness. UGT2B7 activity or inhibition directly connects with the response to various drugs underlining its significance in pharmacogenomics and personalized medicine approaches.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

UDP-glucuronosyltransferase (UGT) that catalyzes phase II biotransformation reactions in which lipophilic substrates are conjugated with glucuronic acid to increase the metabolite's water solubility, thereby facilitating excretion into either the urine or bile (PubMed : 10702251, PubMed : 15470161, PubMed : 15472229, PubMed : 17442341, PubMed : 18674515, PubMed : 18719240, PubMed : 19022937, PubMed : 23288867, PubMed : 23756265, PubMed : 26220143). Essential for the elimination and detoxification of drugs, xenobiotics and endogenous compounds (PubMed : 15470161, PubMed : 18674515, PubMed : 23756265). Catalyzes the glucuronidation of endogenous steroid hormones such as androgens (epitestosterone, androsterone) and estrogens (estradiol, epiestradiol, estriol, catechol estrogens) (PubMed : 15472229, PubMed : 17442341, PubMed : 18719240, PubMed : 19022937, PubMed : 2159463, PubMed : 23288867, PubMed : 26220143). Also regulates the levels of retinoic acid, a major metabolite of vitamin A involved in apoptosis, cellular growth and differentiation, and embryonic development (PubMed : 10702251). Contributes to bile acid (BA) detoxification by catalyzing the glucuronidation of BA substrates, which are natural detergents for dietary lipids absorption (PubMed : 23756265). Involved in the glucuronidation of the AGTR1 angiotensin receptor antagonist losartan, caderastan and zolarsatan, drugs which can inhibit the effect of angiotensin II (PubMed : 18674515). Also metabolizes mycophenolate, an immunosuppressive agent (PubMed : 15470161).

See full target information UGT2B7

style

left-column

Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Endocrine-related cancer 30: PubMed37800655

2023

Semaphorin 3C promotes de novo steroidogenesis in prostate cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Parvin Yenki,Satyam Bhasin,Liang Liu,Noushin Nabavi,Chi Wing Cheng,Kevin J Tam,James W Peacock,Hans H Adomat,Tabitha Tombe,Ladan Fazli,Larissa Ivanova,Christopher Dusek,Shahram Khosravi,Emma S Tomlinson Guns,Yuzhuo Wang,Ralph Buttyan,Martin E Gleave,Christopher J Ong

Journal of environmental science and health. Part C, Toxicology and carcinogenesis 40:106-118 PubMed35895929

2022

The expression of Phase II drug-metabolizing enzymes in human B-lymphoblastoid TK6 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xilin Li,Yuxi Li,Kylie G Ning,Si Chen,Lei Guo,Jessica A Bonzo,Nan Mei

Hepatic medicine : evidence and research 12:79-92 PubMed32607015

2020

In vitro Differentiation of Human TERT-Transfected Multi-Lineage Progenitor Cells (MLPC) into Immortalized Hepatocyte-Like Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel P Collins,Joel H Hapke,Rajagopal N Aravalli,Clifford J Steer

Toxicology and applied pharmacology 359:91-101 PubMed30248416

2018

Emodin-induced hepatotoxicity was exacerbated by probenecid through inhibiting UGTs and MRP2.

Applications

Unspecified application

Species

Unspecified reactive species

Lili Wu,Yulian Chen,Han Liu,Zhikun Zhan,Zhi Liang,Tao Zhang,Zheng Cai,Ling Ye,Menghua Liu,Jie Zhao,Shuwen Liu,Lan Tang

Frontiers in pharmacology 7:404 PubMed27847477

2016

Reverse of Acute and Chronic Morphine Tolerance by Lithocholic Acid Down-Regulating UGT2B7.

Applications

WB, IHC-P

Species

Mouse, Mouse

Zizhao Yang,Li Li,Haihong Hu,Mingcheng Xu,Jingkai Gu,Zaijie Jim Wang,Lushan Yu,Su Zeng

PloS one 10:e0127524 PubMed26010150

2015

Drug-Metabolizing Activity, Protein and Gene Expression of UDP-Glucuronosyltransferases Are Significantly Altered in Hepatocellular Carcinoma Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Linlin Lu,Juan Zhou,Jian Shi,Xiao-juan Peng,Xiao-xiao Qi,Ying Wang,Fang-Yuan Li,Fu-Yuan Zhou,Liang Liu,Zhong-Qiu Liu

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-UGT2B7 antibody

Western blot - Anti-UGT2B7 antibody (AB126269)

Western blot - Anti-UGT2B7 antibody (AB126269)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Product promise