AB235988

Anti-UGT2B7 antibody

Name: Anti-UGT2B7 antibody (ab235988) | Abcam
Brand: Abcam Limited
SKU: AB235988

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(1 Publication)

Rabbit Polyclonal UGT2B7 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human UGT2B7 aa 200-400.

View Alternative Names

UGTB2B9, UGT2B7, UDP-glucuronosyltransferase 2B7, UDPGT 2B7, UDP-glucuronosyltransferase 2B9, UDPGTh-2, UDPGT 2B9

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-UGT2B7 antibody (AB235988)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-UGT2B7 antibody (AB235988)

4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells stained for UGT2B7 (green) using ab235988 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488^® conjugated Goat Anti-Rabbit IgG (H+L). Counter-stained with DAPI (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGT2B7 antibody (AB235988)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGT2B7 antibody (AB235988)

Paraffin-embedded human colon cancer tissue stained for UGT2B7 using ab235988 at 1/300 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human UGT2B7 aa 200-400. The exact immunogen used to generate this antibody is proprietary information.

P16662

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purity >95%.

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UGT2B7 also known as UDP-glucuronosyltransferase 2B7 is an enzyme that helps in the process of glucuronidation. This enzyme has a molecular mass of approximately 56 kDa. It is part of the UGT2B family and is mainly expressed in the liver but also found in other tissues like the kidney and intestines. UGT2B7 plays an important role in metabolizing diverse compounds including drugs and endogenous substances by adding glucuronic acid to them making them more water-soluble and easier to excrete.

Biological function summary

The glucuronidation function of UGT2B7 is important for detoxifying and eliminating hormones bilirubin and xenobiotics from the body. This enzyme does not function as part of a complex; instead it acts individually. The activity of UGT2B7 aids in maintaining homeostasis by processing toxins and facilitating their clearance from the bloodstream. As a member of the large family of UDP-glucuronosyltransferases UGT2B7 exhibits substrate specificity which ensures efficient metabolism of various substrates.

Pathways

UGT2B7 contributes to the drug metabolism pathway and bilirubin clearance pathway. It works closely with other enzymes such as cytochrome P450s which modify the substrates before UGT2B7 adds glucuronic acid. In the drug metabolism pathway the enzyme participates directly in the phase II reaction stages converting lipophilic drug molecules into hydrophilic metabolites. The enzyme's role in bilirubin clearance assists in preventing the buildup of this compound which could lead to harmful conditions.

Faulty UGT2B7 activity correlates with conditions like Gilbert's syndrome and adverse drug reactions. Gilbert's syndrome a mild genetic condition results from reduced glucuronidation capability leading to increased levels of unconjugated bilirubin. The interaction with drugs can also impact their pharmacokinetics and dynamics resulting in toxicity or ineffectiveness. UGT2B7 activity or inhibition directly connects with the response to various drugs underlining its significance in pharmacogenomics and personalized medicine approaches.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

UDP-glucuronosyltransferase (UGT) that catalyzes phase II biotransformation reactions in which lipophilic substrates are conjugated with glucuronic acid to increase the metabolite's water solubility, thereby facilitating excretion into either the urine or bile (PubMed : 10702251, PubMed : 15470161, PubMed : 15472229, PubMed : 17442341, PubMed : 18674515, PubMed : 18719240, PubMed : 19022937, PubMed : 23288867, PubMed : 23756265, PubMed : 26220143). Essential for the elimination and detoxification of drugs, xenobiotics and endogenous compounds (PubMed : 15470161, PubMed : 18674515, PubMed : 23756265). Catalyzes the glucuronidation of endogenous steroid hormones such as androgens (epitestosterone, androsterone) and estrogens (estradiol, epiestradiol, estriol, catechol estrogens) (PubMed : 15472229, PubMed : 17442341, PubMed : 18719240, PubMed : 19022937, PubMed : 2159463, PubMed : 23288867, PubMed : 26220143). Also regulates the levels of retinoic acid, a major metabolite of vitamin A involved in apoptosis, cellular growth and differentiation, and embryonic development (PubMed : 10702251). Contributes to bile acid (BA) detoxification by catalyzing the glucuronidation of BA substrates, which are natural detergents for dietary lipids absorption (PubMed : 23756265). Involved in the glucuronidation of the AGTR1 angiotensin receptor antagonist losartan, caderastan and zolarsatan, drugs which can inhibit the effect of angiotensin II (PubMed : 18674515). Also metabolizes mycophenolate, an immunosuppressive agent (PubMed : 15470161).

See full target information UGT2B7

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular neurobiology 61:1936-1952 PubMed37819429

2023

Teriflunomide Promotes Blood-Brain Barrier Integrity by Upregulating Claudin-1 via the Wnt/β-catenin Signaling Pathway in Multiple Sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yipeng Zhao,Chen Chen,Xiuqing Xiao,Ling Fang,Xi Cheng,Yanyu Chang,Fuhua Peng,Jingqi Wang,Shishi Shen,Shilin Wu,Yiying Huang,Wei Cai,Linli Zhou,Wei Qiu

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-UGT2B7 antibody

Immunocytochemistry/ Immunofluorescence - Anti-UGT2B7 antibody (AB235988)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGT2B7 antibody (AB235988)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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