JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB181245

Anti-UGT3A1 antibody [EPR13723]

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal UGT3A1 antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

UDP-glucuronosyltransferase 3A1, UDPGT 3A1, UGT3A1

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGT3A1 antibody [EPR13723] (AB181245)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGT3A1 antibody [EPR13723] (AB181245)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling UGT3A1 with ab181245 at a 1/100 dilution. Counter stained with hematoxylin. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGT3A1 antibody [EPR13723] (AB181245)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGT3A1 antibody [EPR13723] (AB181245)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling UGT3A1 with ab181245 at a 1/100 dilution. Counter stained with hematoxylin.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-UGT3A1 antibody [EPR13723] (AB181245)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-UGT3A1 antibody [EPR13723] (AB181245)

Immunofluorescence analysis of paraformaldehyde-fixed HepG2 cells labeling UGT3A1 with ab181245 at a 1/500 dilution. As secondary antibody goat anti rabbit IgG (Alexa Fluor®555) was used at a 1/200. In blue DAPI staining.

Immunoprecipitation - Anti-UGT3A1 antibody [EPR13723] (AB181245)
  • IP

Supplier Data

Immunoprecipitation - Anti-UGT3A1 antibody [EPR13723] (AB181245)

Western blot analysis on immunoprecipitation from 1) Human fetal liver lysate or 2) negative control, labeling UGT3A1 using ab181245 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-UGT3A1 antibody [EPR13723] (ab181245)

Predicted band size: 59 kDa

Observed band size: 59 kDa

true

Western blot - Anti-UGT3A1 antibody [EPR13723] (AB181245)
  • WB

Supplier Data

Western blot - Anti-UGT3A1 antibody [EPR13723] (AB181245)

All lanes:

Western blot - Anti-UGT3A1 antibody [EPR13723] (ab181245) at 1/1000 dilution

Lane 1:

Human fetal liver lysate at 20 µg

Lane 2:

Human fetal kidney lysate at 20 µg

Lane 3:

Human testis lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/500 dilution

Predicted band size: 59 kDa

Observed band size: 59 kDa

true

Western blot - Anti-UGT3A1 antibody [EPR13723] (AB181245)
  • WB

Supplier Data

Western blot - Anti-UGT3A1 antibody [EPR13723] (AB181245)

All lanes:

Western blot - Anti-UGT3A1 antibody [EPR13723] (ab181245) at 1/1000 dilution

All lanes:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 59 kDa

Observed band size: 59 kDa

true

  • Carrier free

    Anti-UGT3A1 antibody [EPR13723] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13723

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/40 - 1/60", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UGT3A1 also known as UDP-glucuronosyltransferase 3A1 is an enzyme involved in the process of glucuronidation which adds glucuronic acid to substances aiding in their solubility and excretion from the body. This enzyme has a molecular mass of approximately 60 kDa. UGT3A1 is primarily expressed in the liver with some expression in other tissues such as the kidney and intestine. It acts by conjugating a variety of lipophilic compounds including drugs toxins and endogenous compounds like hormones and bilirubin facilitating their elimination.
Biological function summary

This enzyme plays an important role in detoxification and metabolism processes. UGT3A1 is part of the larger UDP-glucuronosyltransferase family which participates in phase II of drug metabolism. It does not function individually but as a homodimer offering structural support for catalytic activity. By making hydrophobic molecules more water-soluble UGT3A1 reduces their biological activity and assists in their removal from the body maintaining homeostasis.

Pathways

UGT3A1 is integral to the glucuronidation pathway a critical phase II metabolic process. This pathway is essential for the clearance of a wide range of substrates including therapeutic drugs and endogenous substrates. UGT3A1 interacts with the cytochrome P450 family which primarily deal with phase I of drug metabolism. Together they constitute a comprehensive metabolic framework essential for biotransformation of many xenobiotics and endogenous substances.

UGT3A1 has been associated with altered drug metabolism potentially influencing drug clearance and leading to adverse drug reactions. Additionally variations in UGT3A1 activity might contribute to disorders involving hormone imbalances due to its role in hormone glucuronidation. Polymorphisms in UGT3A1 gene may impact its expression and function linking it indirectly with CYP3A4 another pivotal enzyme in drug metabolism affecting overall pharmaceutical efficacy and safety.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

UDP-glucuronosyltransferases catalyze phase II biotransformation reactions in which lipophilic substrates are conjugated with glucuronic acid to increase water solubility and enhance excretion. They are of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds (By similarity).
See full target information UGT3A1
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 20:626 PubMed36578050

2022

miR-4443 promotes radiation resistance of esophageal squamous cell carcinoma via targeting PTPRJ.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaobo Shi,Xiaoxiao Liu,Shan Huang,Yu Hao,Shupei Pan,Yue Ke,Wei Guo,Yuchen Wang,Hongbing Ma
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB170858

Anti-UGT1A1 antibody [EPR9592]

RabMAb|Recombinant

Western blot - Anti-UGT1A1 antibody [EPR9592] (AB170858)

  • 4
  • 1
Primary Antibodies

AB150353

Anti-RBMS1 antibody [EPR9825(B)]

RabMAb|Recombinant

Western blot - Anti-RBMS1 antibody [EPR9825(B)] (AB150353)

  • 0
  • 0
Primary Antibodies

AB250171

Anti-NUDT19 antibody [EPR13162-106] - BSA and Azide free

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-NUDT19 antibody [EPR13162-106] - BSA and Azide free (AB250171)

  • 0
  • 0
Primary Antibodies

AB181225

Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] (AB181225)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com