Anti-UGT3A1 antibody [EPR13723] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGT3A1 antibody [EPR13723] - BSA and Azide free (AB250458)

This data was developed using ab181245, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded, Human liver tissue labeling UGT3A1 with ab181245 at a 1/100 dilution. Counter stained with hematoxylin. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGT3A1 antibody [EPR13723] - BSA and Azide free (AB250458)

This data was developed using ab181245, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded, Human kidney tissue labeling UGT3A1 with ab181245 at a 1/100 dilution. Counter stained with hematoxylin.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-UGT3A1 antibody [EPR13723] - BSA and Azide free (AB250458)

This data was developed using ab181245, the same antibody clone in a different buffer formulation.

Immunofluorescence analysis of, paraformaldehyde-fixed, HepG2 cells labeling UGT3A1 with ab181245 at a 1/500 dilution. As secondary antibody goat anti rabbit IgG (Alexa Fluor®555) was used at a 1/200. In blue DAPI staining.

• IP

Supplier Data

Immunoprecipitation - Anti-UGT3A1 antibody [EPR13723] - BSA and Azide free (AB250458)

This data was developed using ab181245, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation from 1) Human fetal liver lysate or 2) negative control, labeling UGT3A1 using ab181245 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-UGT3A1 antibody [EPR13723] (<a href='/en-us/products/primary-antibodies/ugt3a1-antibody-epr13723-ab181245'>ab181245</a>)

Predicted band size: 59 kDa

Observed band size: 59 kDa

true

• WB

Supplier Data

Western blot - Anti-UGT3A1 antibody [EPR13723] - BSA and Azide free (AB250458)

This data was developed using ab181245, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-UGT3A1 antibody [EPR13723] (<a href='/en-us/products/primary-antibodies/ugt3a1-antibody-epr13723-ab181245'>ab181245</a>) at 1/1000 dilution

Lane 1:

Human fetal liver lysate at 20 µg

Lane 2:

Human fetal kidney lysate at 20 µg

Lane 3:

Human testis lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/500 dilution

Predicted band size: 59 kDa

Observed band size: 59 kDa

true

• WB

Supplier Data

Western blot - Anti-UGT3A1 antibody [EPR13723] - BSA and Azide free (AB250458)

This data was developed using ab181245, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-UGT3A1 antibody [EPR13723] (<a href='/en-us/products/primary-antibodies/ugt3a1-antibody-epr13723-ab181245'>ab181245</a>) at 1/1000 dilution

All lanes:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 59 kDa

Observed band size: 59 kDa

true