Anti-UHRF1 antibody [3A11]

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-UHRF1 antibody [3A11] (AB57083)

ICC/IF image of ab57083 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab57083, 5μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

This image was generated using the ascites version of the product.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UHRF1 antibody [3A11] (AB57083)

UHRF1 antibody (ab57083) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human endometrium.

This image was generated using the ascites version of the product.

• Flow Cyt

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Flow Cytometry - Anti-UHRF1 antibody [3A11] (AB57083)

Overlay histogram showing MCF7 cells stained with ab57083 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57083, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

This image was generated using the ascites version of the product.

• WB

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Western blot - Anti-UHRF1 antibody [3A11] (AB57083)

UHRF1 antibody (ab57083) at 1ug/lane + HeLa cell lysate at 25ug/lane.

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-UHRF1 antibody [3A11] (ab57083)

Predicted band size: 89 kDa

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• WB

CiteAb

Western blot - Anti-UHRF1 antibody [3A11] (AB57083)

UHRF1 western blot using anti-UHRF1 antibody [3A11] ab57083. Publication image and figure legend from Nakamura, K., Baba, Y., et al., 2016, Oncotarget, PubMed 27507047.

ab57083 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab57083 please see the product overview.

Knockdown of UHRF1 with siRNA caused upregulation of LINE-1 methylation levels in ESCC cell lines(A) Western blot analysis of UHRF1 expression in cells transfected with siUHRF1. (B, C) Changes in DNA methylation after cotransfection with siUHRF1 and EGFP-MBD1 vector. (D) EGFP-MBD1 expression after cotransfection, as determined by western blot.

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