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Rabbit Polyclonal UHRF1 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human UHRF1 aa 50-550.

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Images

Western blot - Anti-UHRF1 antibody (AB153972), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-UHRF1 antibody (AB153972), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UHRF1 antibody (AB153972), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human UHRF1 aa 50-550. The exact immunogen used to generate this antibody is proprietary information. Database link Q96T88

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIHC-P
Human
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/500 - 1/3000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100 - 1/1000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100 - 1/1000

Notes

Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).

Associated Products

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Target data

Function

Multidomain protein that acts as a key epigenetic regulator by bridging DNA methylation and chromatin modification. Specifically recognizes and binds hemimethylated DNA at replication forks via its YDG domain and recruits DNMT1 methyltransferase to ensure faithful propagation of the DNA methylation patterns through DNA replication. In addition to its role in maintenance of DNA methylation, also plays a key role in chromatin modification: through its tudor-like regions and PHD-type zinc fingers, specifically recognizes and binds histone H3 trimethylated at 'Lys-9' (H3K9me3) and unmethylated at 'Arg-2' (H3R2me0), respectively, and recruits chromatin proteins. Enriched in pericentric heterochromatin where it recruits different chromatin modifiers required for this chromatin replication. Also localizes to euchromatic regions where it negatively regulates transcription possibly by impacting DNA methylation and histone modifications. Has E3 ubiquitin-protein ligase activity by mediating the ubiquitination of target proteins such as histone H3 and PML. It is still unclear how E3 ubiquitin-protein ligase activity is related to its role in chromatin in vivo. Plays a role in DNA repair by cooperating with UHRF2 to ensure recruitment of FANCD2 to interstrand cross-links (ICLs) leading to FANCD2 activation. Acts as a critical player of proper spindle architecture by catalyzing the 'Lys-63'-linked ubiquitination of KIF11, thereby controlling KIF11 localization on the spindle (PubMed:37728657).

Alternative names

Recommended products

Rabbit Polyclonal UHRF1 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human UHRF1 aa 50-550.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human UHRF1 aa 50-550. The exact immunogen used to generate this antibody is proprietary information. Database link Q96T88
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

UHRF1 also known as ICBP90 or Np95 is a nuclear protein playing key roles in epigenetic regulation. It weighs approximately 90 kDa. UHRF1 is expressed in various tissues most highly in proliferating cells. Functionally UHRF1 recognizes hemimethylated DNA and recruits DNA methyltransferase 1 (DNMT1) to maintain DNA methylation after replication. This ability to interact with both DNA and chromatin makes UHRF1 central to chromatin modification processes.

Biological function summary

UHRF1 engages in preserving the DNA methylation landscape ensuring proper gene expression patterns. It is part of a multi-protein complex and processes both histone modification and DNA methylation. Its presence is critical for gene silencing through the recruitment of DNMT1 and histone deacetylases. UHRF1 therefore influences regulatory regions of genes important for cell cycle progression and development.

Pathways

UHRF1 integrates into methylation-dependent gene silencing and cell cycle regulation. It actively participates in DNA damage repair pathways by modulating the p53 pathway aiding cell survival post-DNA damage. UHRF1 closely interacts with epigenetic regulators such as DNMT1 and histone deacetylases bridging methylation and chromatin remodeling to ensure proper cell cycle and transcriptional control.

Associated diseases and disorders

UHRF1's dysregulation links to cancers including breast and lung cancer due to its role in maintaining aberrant methylation. Abnormal UHRF1 expression leads to improper silencing of tumor suppressor genes. UHRF1's interaction with DNMT1 and modulation of pathways involving p53 are important for cancer cell proliferation making it a potential target for cancer therapies.

Product promise

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3 product images

  • Western blot - Anti-UHRF1 antibody (ab153972), expandable thumbnail

    Western blot - Anti-UHRF1 antibody (ab153972)

    7.5% SDS PAGE

    All lanes: Western blot - Anti-UHRF1 antibody (ab153972) at 1/2000 dilution

    All lanes: HeLa whole cell lysate at 30 µg

    Predicted band size: 89 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-UHRF1 antibody (ab153972), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-UHRF1 antibody (ab153972)

    Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling UHRF1 with ab153972 at 1/500 dilution (Green). Alpha-tubulin filaments were labeled Red.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UHRF1 antibody (ab153972), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UHRF1 antibody (ab153972)

    Immunohistochemical analysis of paraffin-embedded Human BT483 xenograft labeling UHRF1 with ab153972 at 1/250 dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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