Rabbit Recombinant Monoclonal UHRF1 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (AB240388), expandable thumbnail](https://content.abcam.com/products/images/uhrf1-antibody-epr18803-11-bsa-and-azide-free-ab240388--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img132737.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (AB240388)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (AB240388), expandable thumbnail](https://content.abcam.com/products/images/uhrf1-antibody-epr18803-11-bsa-and-azide-free-ab240388--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img94617.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (AB240388)")
![Immunoprecipitation - Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (AB240388), expandable thumbnail](https://content.abcam.com/products/images/uhrf1-antibody-epr18803-11-bsa-and-azide-free-ab240388--immunoprecipitation-img15618.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (AB240388)")
![Immunocytochemistry/ Immunofluorescence - Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (AB240388), expandable thumbnail](https://content.abcam.com/products/images/uhrf1-antibody-epr18803-11-bsa-and-azide-free-ab240388--immunocytochemistry-immunofluorescence-img23448.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (AB240388)")
![Immunocytochemistry/ Immunofluorescence - Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (AB240388), expandable thumbnail](https://content.abcam.com/products/images/uhrf1-antibody-epr18803-11-bsa-and-azide-free-ab240388--immunocytochemistry-immunofluorescence-img21934.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (AB240388)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|---|
| Human | Tested | Expected | Tested | Tested | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Recommended for human only. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Recommended for human only. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Multidomain protein that acts as a key epigenetic regulator by bridging DNA methylation and chromatin modification. Specifically recognizes and binds hemimethylated DNA at replication forks via its YDG domain and recruits DNMT1 methyltransferase to ensure faithful propagation of the DNA methylation patterns through DNA replication. In addition to its role in maintenance of DNA methylation, also plays a key role in chromatin modification: through its tudor-like regions and PHD-type zinc fingers, specifically recognizes and binds histone H3 trimethylated at 'Lys-9' (H3K9me3) and unmethylated at 'Arg-2' (H3R2me0), respectively, and recruits chromatin proteins. Enriched in pericentric heterochromatin where it recruits different chromatin modifiers required for this chromatin replication. Also localizes to euchromatic regions where it negatively regulates transcription possibly by impacting DNA methylation and histone modifications. Has E3 ubiquitin-protein ligase activity by mediating the ubiquitination of target proteins such as histone H3 and PML. It is still unclear how E3 ubiquitin-protein ligase activity is related to its role in chromatin in vivo. Plays a role in DNA repair by cooperating with UHRF2 to ensure recruitment of FANCD2 to interstrand cross-links (ICLs) leading to FANCD2 activation. Acts as a critical player of proper spindle architecture by catalyzing the 'Lys-63'-linked ubiquitination of KIF11, thereby controlling KIF11 localization on the spindle (PubMed:37728657).
Alternative names
ICBP90, NP95, RNF106, UHRF1, E3 ubiquitin-protein ligase UHRF1, Inverted CCAAT box-binding protein of 90 kDa, Nuclear protein 95, Nuclear zinc finger protein Np95, RING finger protein 106, RING-type E3 ubiquitin transferase UHRF1, Transcription factor ICBP90, Ubiquitin-like PHD and RING finger domain-containing protein 1, Ubiquitin-like-containing PHD and RING finger domains protein 1, HuNp95, hNp95, hUHRF1
Recommended products
Rabbit Recombinant Monoclonal UHRF1 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR18803-11
- Purification technique
- Affinity purification Protein A
- Specificity
UHRF1 is aboundant in tissues like thymus, bone marrow. For other tissues with low expression level, please upload higher amount of tissue lysate or use lower antibody dilution.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab240388 is the carrier-free version of Anti-UHRF1 antibody [EPR18803-11] ab213223.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
UHRF1 also known as ICBP90 or Np95 is a nuclear protein playing key roles in epigenetic regulation. It weighs approximately 90 kDa. UHRF1 is expressed in various tissues most highly in proliferating cells. Functionally UHRF1 recognizes hemimethylated DNA and recruits DNA methyltransferase 1 (DNMT1) to maintain DNA methylation after replication. This ability to interact with both DNA and chromatin makes UHRF1 central to chromatin modification processes.
- Biological function summary
UHRF1 engages in preserving the DNA methylation landscape ensuring proper gene expression patterns. It is part of a multi-protein complex and processes both histone modification and DNA methylation. Its presence is critical for gene silencing through the recruitment of DNMT1 and histone deacetylases. UHRF1 therefore influences regulatory regions of genes important for cell cycle progression and development.
- Pathways
UHRF1 integrates into methylation-dependent gene silencing and cell cycle regulation. It actively participates in DNA damage repair pathways by modulating the p53 pathway aiding cell survival post-DNA damage. UHRF1 closely interacts with epigenetic regulators such as DNMT1 and histone deacetylases bridging methylation and chromatin remodeling to ensure proper cell cycle and transcriptional control.
- Associated diseases and disorders
UHRF1's dysregulation links to cancers including breast and lung cancer due to its role in maintaining aberrant methylation. Abnormal UHRF1 expression leads to improper silencing of tumor suppressor genes. UHRF1's interaction with DNMT1 and modulation of pathways involving p53 are important for cancer cell proliferation making it a potential target for cancer therapies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (ab240388), expandable thumbnail](https://content.abcam.com/products/images/uhrf1-antibody-epr18803-11-bsa-and-azide-free-ab240388--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img132737.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (ab240388)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (ab240388)
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling UHRF1 with Anti-UHRF1 antibody [EPR18803-11] ab213223 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Nucleus staining on Human tonsil germinal center is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-UHRF1 antibody [EPR18803-11] ab213223).
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (ab240388), expandable thumbnail](https://content.abcam.com/products/images/uhrf1-antibody-epr18803-11-bsa-and-azide-free-ab240388--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img94617.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (ab240388)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (ab240388)
Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling UHRF1 with Anti-UHRF1 antibody [EPR18803-11] ab213223 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Nucleus staining on Human colon cancer is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-UHRF1 antibody [EPR18803-11] ab213223).
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Immunoprecipitation - Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (ab240388), expandable thumbnail](https://content.abcam.com/products/images/uhrf1-antibody-epr18803-11-bsa-and-azide-free-ab240388--immunoprecipitation-img15618.jpg "Immunoprecipitation - Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (ab240388)")
Immunoprecipitation - Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (ab240388)
UHRF1 was immunoprecipitated from 0.35 mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with Anti-UHRF1 antibody [EPR18803-11] ab213223 at 1/30 dilution.
Western blot was performed from the immunoprecipitate using Anti-UHRF1 antibody [EPR18803-11] ab213223 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.
Lane 1: HeLa whole cell lysate, 10μg (Input).
Lane 2: Anti-UHRF1 antibody [EPR18803-11] ab213223 IP in HeLa whole cell lysate.
Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-UHRF1 antibody [EPR18803-11] ab213223 in HeLa whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-UHRF1 antibody [EPR18803-11] ab213223).
All lanes: Immunoprecipitation - Anti-UHRF1 antibody [EPR18803-11] (Anti-UHRF1 antibody [EPR18803-11] ab213223)
Predicted band size: 89 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (ab240388), expandable thumbnail](https://content.abcam.com/products/images/uhrf1-antibody-epr18803-11-bsa-and-azide-free-ab240388--immunocytochemistry-immunofluorescence-img23448.jpg "Immunocytochemistry/ Immunofluorescence - Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (ab240388)")
Immunocytochemistry/ Immunofluorescence - Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (ab240388)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cell line from peripheral blood) cells labeling UHRF1 with Anti-UHRF1 antibody [EPR18803-11] ab213223 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor®488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing nuclear staining on HeLa cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (Goat anti-Mouse IgG (Alexa Fluor® 594)) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: Anti-UHRF1 antibody [EPR18803-11] ab213223 at 1/500 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-UHRF1 antibody [EPR18803-11] ab213223).
![Immunocytochemistry/ Immunofluorescence - Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (ab240388), expandable thumbnail](https://content.abcam.com/products/images/uhrf1-antibody-epr18803-11-bsa-and-azide-free-ab240388--immunocytochemistry-immunofluorescence-img21934.jpg "Immunocytochemistry/ Immunofluorescence - Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (ab240388)")
Immunocytochemistry/ Immunofluorescence - Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (ab240388)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling UHRF1 with Anti-UHRF1 antibody [EPR18803-11] ab213223 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor®488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing nuclear staining on HeLa cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (Goat anti-Mouse IgG (Alexa Fluor® 594)) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: Anti-UHRF1 antibody [EPR18803-11] ab213223 at 1/500 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-UHRF1 antibody [EPR18803-11] ab213223).
![Flow Cytometry (Intracellular) - Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (ab240388), expandable thumbnail](https://content.abcam.com/products/images/uhrf1-antibody-epr18803-11-bsa-and-azide-free-ab240388--flow-cytometry-intracellular-img414412.jpg "Flow Cytometry (Intracellular) - Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (ab240388)")
Flow Cytometry (Intracellular) - Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (ab240388)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed Jurkat (Human T cell leukemia cell line from peripheral blood) cells labeling UHRF1 with Anti-UHRF1 antibody [EPR18803-11] ab213223 at 1/70 dilution (red) compared with a Rabbit IgG,monoclonal [EPR25A] - Isotype control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-UHRF1 antibody [EPR18803-11] ab213223).
![Western blot - Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (ab240388), expandable thumbnail](https://content.abcam.com/products/images/uhrf1-antibody-epr18803-11-bsa-and-azide-free-ab240388--western-blot-img415953.jpg "Western blot - Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (ab240388)")
Western blot - Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (ab240388)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-UHRF1 antibody [EPR18803-11] ab213223).
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
UHRF1 has two isoforms.
UHRF1 undergo degradation under normal conditions as well as in response to DNA damage. (PMID: 23297342, PMID: 36593255)All lanes: Western blot - Anti-UHRF1 antibody [EPR18803-11] (Anti-UHRF1 antibody [EPR18803-11] ab213223) at 1/1000 dilution
Lane 1: Human colon tissue lysate at 20 µg
Lane 2: Human thymus tissue lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/20000 dilution
Predicted band size: 89 kDa
Exposure time: 20s
![Western blot - Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (ab240388), expandable thumbnail](https://content.abcam.com/products/images/uhrf1-antibody-epr18803-11-bsa-and-azide-free-ab240388--western-blot-img415952.jpg "Western blot - Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (ab240388)")
Western blot - Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (ab240388)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-UHRF1 antibody [EPR18803-11] ab213223).
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as GAPDH loading control.
UHRF1 has two isoforms.
UHRF1 undergo degradation under normal conditions as well as in response to DNA damage. (PMID: 23297342, PMID: 36593255)All lanes: Western blot - Anti-UHRF1 antibody [EPR18803-11] (Anti-UHRF1 antibody [EPR18803-11] ab213223) at 1/1000 dilution
Lane 1: Human brain tissue lysate at 20 µg
Lane 2: Human kidney tissue lysate at 20 µg
Lane 3: Human spleen tissue lysate at 20 µg
Lane 4: Human colon tissue lysate at 20 µg
Lane 5: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 6: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 7: U-937 (Human histiocytic lymphoma monocyte) whole cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/20000 dilution
Predicted band size: 89 kDa
Exposure time: 20s
![Flow Cytometry (Intracellular) - Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (ab240388), expandable thumbnail](https://content.abcam.com/products/images/uhrf1-antibody-epr18803-11-bsa-and-azide-free-ab240388--flow-cytometry-intracellular-img414412.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (ab240388)")
![Western blot - Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (ab240388), expandable thumbnail](https://content.abcam.com/products/images/uhrf1-antibody-epr18803-11-bsa-and-azide-free-ab240388--western-blot-img415953.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (ab240388)")
![Western blot - Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (ab240388), expandable thumbnail](https://content.abcam.com/products/images/uhrf1-antibody-epr18803-11-bsa-and-azide-free-ab240388--western-blot-img415952.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (ab240388)")
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