Anti-UHRF1 antibody [EPR18803] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UHRF1 antibody [EPR18803] - BSA and Azide free (AB251181)

This data was developed using ab194236, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling UHRF1 with ab194236 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Nucleus staining on lymphocytes of Human tonsil is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UHRF1 antibody [EPR18803] - BSA and Azide free (AB251181)

This data was developed using ab194236, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling UHRF1 with ab194236 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Nucleus staining on the epithelial cells of Human colon is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IP

Supplier Data

Immunoprecipitation - Anti-UHRF1 antibody [EPR18803] - BSA and Azide free (AB251181)

This data was developed using ab194236, the same antibody clone in a different buffer formulation.

UHRF1 was immunoprecipitated from 1mg of HepG2 (Human liver hepatocellular carcinoma) whole cell lysate with ab194236 at 1/20 dilution.

Western blot was performed from the immunoprecipitate using ab194236 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1 : HepG2 whole cell lysate 10ug (Input).

Lane 2 : ab194236 IP in HepG2 whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab194236 in HepG2 whole cell lysate.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-UHRF1 antibody [EPR18803] (<a href='/en-us/products/primary-antibodies/uhrf1-antibody-epr18803-ab194236'>ab194236</a>)

Predicted band size: 89 kDa

Observed band size: 97 kDa

false

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UHRF1 antibody [EPR18803] - BSA and Azide free (AB251181)

This data was developed using ab194236, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling UHRF1 with ab194236 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Nucleus staining on lymphocytes of rat spleen is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• WB

Supplier Data

Western blot - Anti-UHRF1 antibody [EPR18803] - BSA and Azide free (AB251181)

This data was developed using ab194236, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure times : Lanes 1-2 : 10 seconds; Lane 3 : 3 minutes.

The molecular weight observed is consistent with what has been described in the literature (PMID : 12530060).

All lanes:

Western blot - Anti-UHRF1 antibody [EPR18803] (<a href='/en-us/products/primary-antibodies/uhrf1-antibody-epr18803-ab194236'>ab194236</a>) at 1/5000 dilution

Lane 1:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 20 µg

Lane 3:

HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 89 kDa

Observed band size: 97 kDa

false

• WB

Lab

Western blot - Anti-UHRF1 antibody [EPR18803] - BSA and Azide free (AB251181)

This data was developed using ab194236, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM /TBST. UHRF1 has two isoforms. UHRF1 undergo degradation under normal conditions as well as in response to DNA damage. (PMID : 23297342, PMID : 36593255)

All lanes:

Western blot - Anti-UHRF1 antibody [EPR18803] (<a href='/en-us/products/primary-antibodies/uhrf1-antibody-epr18803-ab194236'>ab194236</a>) at 1/1000 dilution

Lane 1:

Human colon tissue lysate at 20 µg

Lane 2:

Human breast tissue lysate at 20 µg

Lane 3:

Human thymus tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/20000 dilution

Predicted band size: 89 kDa

false

Exposure time: 180s

• WB

Lab

Western blot - Anti-UHRF1 antibody [EPR18803] - BSA and Azide free (AB251181)

This data was developed using ab194236, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM /TBST. ab181602 was used as GAPDH loading control. UHRF1 has two isoforms. UHRF1 undergo degradation under normal conditions as well as in response to DNA damage. (PMID : 23297342, PMID : 36593255)

All lanes:

Western blot - Anti-UHRF1 antibody [EPR18803] (<a href='/en-us/products/primary-antibodies/uhrf1-antibody-epr18803-ab194236'>ab194236</a>) at 1/1000 dilution

Lane 1:

Human brain tissue lysate at 20 µg

Lane 2:

Human kidney tissue lysate at 20 µg

Lane 3:

Human spleen tissue lysate at 20 µg

Lane 4:

Human colon tissue lysate at 20 µg

Lane 5:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 6:

HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 7:

U-937 (Human histiocytic lymphoma monocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/20000 dilution

Predicted band size: 89 kDa

false

Exposure time: 20s