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AB303552

Anti-UHRF1 antibody [EPR25061-269]

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Rabbit Recombinant Monoclonal UHRF1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IP and reacts with Mouse samples.

View Alternative Names

Np95, Uhrf1, E3 ubiquitin-protein ligase UHRF1, Nuclear protein 95, Nuclear zinc finger protein Np95, RING-type E3 ubiquitin transferase UHRF1, Ubiquitin-like PHD and RING finger domain-containing protein 1, Ubiquitin-like-containing PHD and RING finger domains protein 1, mUhrf1

6 Images
Immunocytochemistry/ Immunofluorescence - Anti-UHRF1 antibody [EPR25061-269] (AB303552)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-UHRF1 antibody [EPR25061-269] (AB303552)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized F9 (mouse embryonal carcinoma epithelial cell) cells labelling UHRF1 with ab303552 at 1/500 (1.066 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing nuclear staining in F9 cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

Flow Cytometry (Intracellular) - Anti-UHRF1 antibody [EPR25061-269] (AB303552)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-UHRF1 antibody [EPR25061-269] (AB303552)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized F9 (mouse embryonal carcinoma epithelial cell) cells labelling UHRF1 with ab303552 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

Immunoprecipitation - Anti-UHRF1 antibody [EPR25061-269] (AB303552)
  • IP

Supplier Data

Immunoprecipitation - Anti-UHRF1 antibody [EPR25061-269] (AB303552)

UHRF1 was immunoprecipitated from 0.35 mg mouse thymus tissue lysate 10 ug with ab303552 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab303552 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution. Lane 1 : Mouse thymus tissue lysate 10 ug. Lane 2 : ab303552 IP in mouse thymus tissue lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab303552 in mouse thymus tissue lysate. Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 111 seconds.

All lanes:

Immunoprecipitation - Anti-UHRF1 antibody [EPR25061-269] (ab303552) at 1/1000 dilution

All lanes:

Mouse thymus tissue lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Immunoprecipitation - Anti-UHRF1 antibody [EPR25061-269] (AB303552)
  • IP

Supplier Data

Immunoprecipitation - Anti-UHRF1 antibody [EPR25061-269] (AB303552)

UHRF1 was immunoprecipitated from 0.35 mg F9 (mouse embryonal carcinoma epithelial cell), whole cell lysate 10 ug with ab303552 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab303552 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : F9 whole cell lysate 10 ug Lane 2 : ab303552 IP in F9 whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab303552 in F9 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 111 seconds.

All lanes:

Immunoprecipitation - Anti-UHRF1 antibody [EPR25061-269] (ab303552) at 1/1000 dilution

All lanes:

F9 (mouse embryonal carcinoma epithelial cell), whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 111s

Western blot - Anti-UHRF1 antibody [EPR25061-269] (AB303552)
  • WB

Supplier Data

Western blot - Anti-UHRF1 antibody [EPR25061-269] (AB303552)

This data was developed using ab303552, the same antibody clone in a different buffer formulation.  Blocking and dilution buffer and concentration : 5% NFDM/TBST.  Exposure time : Lane 1-2 : 37 seconds Lane 3 : 92 seconds The identity of the bands between 30 kDa and 50 kDa is unknown.

All lanes:

Western blot - Anti-UHRF1 antibody [EPR25061-269] (ab303552)

Lane 1:

F9 (mouse embryonal carcinoma epithelial cell), whole cell lysate at 40 µg

Lane 2:

ES-D3 (mouse embryonic multipotent stem Cell), whole cell lysate at 40 µg

Lane 3:

C2C12 (mouse myoblasts myoblast), whole cell lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>)

false

Western blot - Anti-UHRF1 antibody [EPR25061-269] (AB303552)
  • WB

Supplier Data

Western blot - Anti-UHRF1 antibody [EPR25061-269] (AB303552)

Blocking and dilution buffer and concentration : 5% NFDM/TBST.  Low expression : brain (PMID : 9880673) The identity of the lower MW band at approximately 37 kDa is unknown.

All lanes:

Western blot - Anti-UHRF1 antibody [EPR25061-269] (ab303552) at 1/1000 dilution

Lane 1:

Mouse thymus tissue lysate at 40 µg

Lane 2:

Mouse brain tissue lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

false

  • Carrier free

    Anti-UHRF1 antibody [EPR25061-269] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25061-269

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

ICC/IF, Flow Cyt (Intra), IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UHRF1 also known as ICBP90 or Np95 is a nuclear protein playing key roles in epigenetic regulation. It weighs approximately 90 kDa. UHRF1 is expressed in various tissues most highly in proliferating cells. Functionally UHRF1 recognizes hemimethylated DNA and recruits DNA methyltransferase 1 (DNMT1) to maintain DNA methylation after replication. This ability to interact with both DNA and chromatin makes UHRF1 central to chromatin modification processes.
Biological function summary

UHRF1 engages in preserving the DNA methylation landscape ensuring proper gene expression patterns. It is part of a multi-protein complex and processes both histone modification and DNA methylation. Its presence is critical for gene silencing through the recruitment of DNMT1 and histone deacetylases. UHRF1 therefore influences regulatory regions of genes important for cell cycle progression and development.

Pathways

UHRF1 integrates into methylation-dependent gene silencing and cell cycle regulation. It actively participates in DNA damage repair pathways by modulating the p53 pathway aiding cell survival post-DNA damage. UHRF1 closely interacts with epigenetic regulators such as DNMT1 and histone deacetylases bridging methylation and chromatin remodeling to ensure proper cell cycle and transcriptional control.

UHRF1's dysregulation links to cancers including breast and lung cancer due to its role in maintaining aberrant methylation. Abnormal UHRF1 expression leads to improper silencing of tumor suppressor genes. UHRF1's interaction with DNMT1 and modulation of pathways involving p53 are important for cancer cell proliferation making it a potential target for cancer therapies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Multidomain protein that acts as a key epigenetic regulator by bridging DNA methylation and chromatin modification. Specifically recognizes and binds hemimethylated DNA at replication forks via its YDG domain and recruits DNMT1 methyltransferase to ensure faithful propagation of the DNA methylation patterns through DNA replication. In addition to its role in maintenance of DNA methylation, also plays a key role in chromatin modification : through its tudor-like regions and PHD-type zinc fingers, specifically recognizes and binds histone H3 trimethylated at 'Lys-9' (H3K9me3) and unmethylated at 'Arg-2' (H3R2me0), respectively, and recruits chromatin proteins. Enriched in pericentric heterochromatin where it recruits different chromatin modifiers required for this chromatin replication. Also localizes to euchromatic regions where it negatively regulates transcription possibly by impacting DNA methylation and histone modifications. Has E3 ubiquitin-protein ligase activity by mediating the ubiquitination of target proteins such as histone H3 and PML. It is still unclear how E3 ubiquitin-protein ligase activity is related to its role in chromatin in vivo. Plays a role in DNA repair by cooperating with UHRF2 to ensure recruitment of FANCD2 to interstrand cross-links (ICLs) leading to FANCD2 activation. Plays a pivotal role in the establishment of correct spindle architecture by catalyzing the 'Lys-63'-linked ubiquitination of KIF11, thereby controlling KIF11 localization on the spindle.
See full target information Uhrf1
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Product promise

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For full details, please see our Terms & Conditions

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