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AB281242

Anti-ULBP1 antibody [EPR22836-277] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal ULBP1 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

N2DL1, RAET1I, ULBP1, UL16-binding protein 1, ALCAN-beta, NKG2D ligand 1, Retinoic acid early transcript 1I, N2DL-1, NKG2DL1

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Sandwich ELISA - Anti-ULBP1 antibody [EPR22836-277] - BSA and Azide free (Capture) (AB281242)
  • sELISA

Lab

Sandwich ELISA - Anti-ULBP1 antibody [EPR22836-277] - BSA and Azide free (Capture) (AB281242)

Sandwich ELISA of ab253682 with the capture antibody ab281242 dilution at 2 µg/mL and detector antibody ab281092 dilution at 0.5 µg/mL.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22836-277

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab281242 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human ULBP1. The recommended pair for sandwich ELISA is:
Capture: ab281242, Human ULBP1 Capture Antibody (unconjugated)
Detector: ab281092, Human ULBP1 Detector Antibody (unconjugated)
The reference range value is 46.88 - 3000 pg/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ULBP1 also known as UL16-binding protein 1 is a member of the MHC class I-related molecules. It weighs approximately 28 kDa. ULBP1 acts mechanically as a ligand for the NKG2D receptor part of the activating receptor on natural killer (NK) cells and certain T cells. It is primarily expressed on the surface of various cells including epithelial and some tumor cells. Its expression can be induced by cellular stress or infection.
Biological function summary

This protein plays a critical role in immune surveillance and response to malignancies and infections. ULBP1 does not form a complex on its own but it works closely with other NKG2D ligands contributing to the immune system's ability to recognize and eliminate abnormal cells. Its presence can enhance immune cell-mediated cytotoxicity indicating its role in immune defense.

Pathways

The protein takes an active part in the immune system activation interacting notably within the NK cell-mediated cytotoxicity pathway. It functions alongside other NKG2D ligands like MICA/MICB to modulate the activity of NK cells and T cells. This connection to the NKG2D pathway ensures a critical interface between innate and adaptive immunity reinforcing defense against infected or tumor cells.

Researchers have noted associations between ULBP1 and cancer progression or immune evasion. Its upregulation on tumor cells can lead to tumor clearance; however some cancers may shed ULBP1 to escape immune detection. In autoimmune diseases such as rheumatoid arthritis altered expression of ULBP1 and its interaction with NKG2D can potentially exacerbate inflammation. These interactions emphasize the balance that ULBP1 maintains in managing immune responses and its implications in pathology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds and activates the KLRK1/NKG2D receptor, mediating natural killer cell cytotoxicity.
See full target information ULBP1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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