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AB88645

Anti-ULBP2 antibody

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(4 Publications)

Mouse Polyclonal ULBP2 antibody. Suitable for Flow Cyt, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ULBP2.

View Alternative Names

N2DL2, RAET1H, UNQ463/PRO791, ULBP2, UL16-binding protein 2, ALCAN-alpha, NKG2D ligand 2, Retinoic acid early transcript 1H, N2DL-2, NKG2DL2

3 Images
Flow Cytometry - Anti-ULBP2 antibody (AB88645)
  • Flow Cyt

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Flow Cytometry - Anti-ULBP2 antibody (AB88645)

ab88645 detecting ULBP2 in ULBP2-expressing 293 cells (Green) compared with negative control 293 cells (Black) by flow cytometry.

Western blot - Anti-ULBP2 antibody (AB88645)
  • WB

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Western blot - Anti-ULBP2 antibody (AB88645)

All lanes:

Western blot - Anti-ULBP2 antibody (ab88645) at 1 µg/mL

All lanes:

Human ovary cancer tissue lysate at 50 µg

Predicted band size: 27 kDa

Observed band size: ~35 kDa

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Western blot - Anti-ULBP2 antibody (AB88645)
  • WB

Unknown

Western blot - Anti-ULBP2 antibody (AB88645)

All lanes:

Western blot - Anti-ULBP2 antibody (ab88645) at 1 µg/mL

Lane 1:

cell lysate from ULBP2 transfected 293T cells at 25 µg

Lane 2:

cell lysate from non transfected 293T cells at 25 µg

Predicted band size: 27 kDa

Observed band size: ~27 kDa,~29 kDa,~33 kDa

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Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, Flow Cyt

applications

Immunogen

Recombinant Full Length Protein corresponding to Human ULBP2.

Q9BZM5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg-isotype-control-ab37355'>ab37355</a> - Mouse polyclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ULBP2 also known as UL16-binding protein 2 and N2DL2 is a glycoprotein with a mass of approximately 30 kDa. It belongs to the family of NKG2D ligands and plays an essential role in the immune response. This protein is expressed on the surface of a variety of cells including epithelial cells fibroblasts and activated immune cells. Its expression can be induced by cellular stress infection or transformation making it an important player in the regulation of the immune system.
Biological function summary

The function of ULBP2 is to facilitate the activation of natural killer (NK) cells and cytotoxic T lymphocytes through binding to the NKG2D receptor. ULBP2 is not a part of a complex but acts independently to trigger immune responses. By interacting with NKG2D ULBP2 promotes the release of cytokines and enhances the cytolytic activity of these immune cells leading to the destruction of infected or transformed cells. This mechanism allows the immune system to efficiently target and eliminate compromised cells.

Pathways

The interaction between ULBP2 and the NKG2D receptor forms a critical part of the immune surveillance pathway. This pathway detects and responds to cellular abnormalities such as tumorigenesis or viral infections. ULBP2's activity relates closely with other NKG2D ligands and components of the immune checkpoint pathways notably involving proteins like MICA and MICB. Additionally ULBP2’s interaction impacts the balance between activating and inhibitory signals within the NK cell cascade thereby influencing immune system homeostasis.

ULBP2 expression has significant implications in cancer and autoimmune conditions. Elevated levels of ULBP2 are frequently associated with various types of cancer where they can either stimulate anti-tumor immunity or be shed to evade immune detection. In this context the protein interacts with NKG2D which is also expressed on tumor-infiltrating lymphocytes. Additionally altered expression of ULBP2 has been observed in inflammatory disorders potentially contributing to tissue damage due to sustained immune activation. Understanding these interactions is important for developing therapeutic strategies targeting ULBP2-related pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds and activates the KLRK1/NKG2D receptor, mediating natural killer cell cytotoxicity.
See full target information ULBP2
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 26:297 PubMed37274476

2023

Soluble NKG2D ligands impair CD8 T cell antitumor function dependent of NKG2D downregulation in neuroblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Zhang,Feifei Luo,Kuiran Dong

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2202519 PubMed36683155

2023

Metabolic Reprogramming of NK Cells by Black Phosphorus Quantum Dots Potentiates Cancer Immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Lizhen He,Jianfu Zhao,Hongjun Li,Bin Xie,Ligeng Xu,Guanning Huang,Ting Liu,Zhen Gu,Tianfeng Chen

Oncoimmunology 9:1845424 PubMed33299656

2020

NK cells in pancreatic cancer demonstrate impaired cytotoxicity and a regulatory IL-10 phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Francesca Marcon,Jianmin Zuo,Hayden Pearce,Samantha Nicol,Sandra Margielewska-Davies,Mustafa Farhat,Brinder Mahon,Gary Middleton,Rachel Brown,Keith J Roberts,Paul Moss

Cancer research 75:1603-14 PubMed25762540

2015

Antitumor immunity triggered by melphalan is potentiated by melanoma cell surface-associated calreticulin.

Applications

WB

Species

Human

Aleksandra M Dudek-Perić,Gabriela B Ferreira,Angelika Muchowicz,Jasper Wouters,Nicole Prada,Shaun Martin,Santeri Kiviluoto,Magdalena Winiarska,Louis Boon,Chantal Mathieu,Joost van den Oord,Marguerite Stas,Marie-Lise Gougeon,Jakub Golab,Abhishek D Garg,Patrizia Agostinis
View all publications
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Product promise

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