AB275023

Anti-ULBP2 antibody [EPR22303-124]

RabMAb
Recombinant
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(5 Publications)

Rabbit Recombinant Monoclonal ULBP2 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 5 publications.

View Alternative Names

N2DL2, RAET1H, UNQ463/PRO791, ULBP2, UL16-binding protein 2, ALCAN-alpha, NKG2D ligand 2, Retinoic acid early transcript 1H, N2DL-2, NKG2DL2

2 Images

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Immunoprecipitation - Anti-ULBP2 antibody [EPR22303-124] (AB275023)

ULBP2 was immunoprecipitated from 0.35 mg BxPC-3 (human pancreas adenocarcinoma epithelial cell), whole cell lysate with ab275023 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab275023 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : BxPC-3 whole cell lysate 10 μg

Lane 2 : ab275023 IP in BxPC-3 whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab275023 inBxPC-3 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 5.5 seconds.

All lanes:

Immunoprecipitation - Anti-ULBP2 antibody [EPR22303-124] (ab275023)

Predicted band size: 27 kDa

Observed band size: 27 kDa

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Supplier Data

Western blot - Anti-ULBP2 antibody [EPR22303-124] (AB275023)

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure times : Lane 1 : 10 seconds Lane 2-7 : 3 minutes.

Negative control : Raji, Daudi (PMID : 12414645).

All lanes:

Western blot - Anti-ULBP2 antibody [EPR22303-124] (ab275023) at 1/1000 dilution

Lane 1:

BxPC-3 (human pancreas adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

MOLT-4 (human lymphoblastic leukemia T lymphoblast), whole cell lysate at 20 µg

Lane 3:

Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate at 20 µg

Lane 4:

Daudi (human Burkitt's lymphoma lymphoblast), whole cell lysate at 20 µg

Lane 5:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 6:

HCT116 (human colorectal carcinoma epithelial cell), whole cell lysate at 20 µg

Lane 7:

U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 27 kDa

Observed band size: 27 kDa

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Carrier free

Anti-ULBP2 antibody [EPR22303-124] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22303-124

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

We have preliminary internal testing data to indicate this antibody weakly cross reacts with recombinant human ULBP5 protein in WB. Please contact us for more information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ULBP2 also known as UL16-binding protein 2 and N2DL2 is a glycoprotein with a mass of approximately 30 kDa. It belongs to the family of NKG2D ligands and plays an essential role in the immune response. This protein is expressed on the surface of a variety of cells including epithelial cells fibroblasts and activated immune cells. Its expression can be induced by cellular stress infection or transformation making it an important player in the regulation of the immune system.

Biological function summary

The function of ULBP2 is to facilitate the activation of natural killer (NK) cells and cytotoxic T lymphocytes through binding to the NKG2D receptor. ULBP2 is not a part of a complex but acts independently to trigger immune responses. By interacting with NKG2D ULBP2 promotes the release of cytokines and enhances the cytolytic activity of these immune cells leading to the destruction of infected or transformed cells. This mechanism allows the immune system to efficiently target and eliminate compromised cells.

Pathways

The interaction between ULBP2 and the NKG2D receptor forms a critical part of the immune surveillance pathway. This pathway detects and responds to cellular abnormalities such as tumorigenesis or viral infections. ULBP2's activity relates closely with other NKG2D ligands and components of the immune checkpoint pathways notably involving proteins like MICA and MICB. Additionally ULBP2’s interaction impacts the balance between activating and inhibitory signals within the NK cell cascade thereby influencing immune system homeostasis.

ULBP2 expression has significant implications in cancer and autoimmune conditions. Elevated levels of ULBP2 are frequently associated with various types of cancer where they can either stimulate anti-tumor immunity or be shed to evade immune detection. In this context the protein interacts with NKG2D which is also expressed on tumor-infiltrating lymphocytes. Additionally altered expression of ULBP2 has been observed in inflammatory disorders potentially contributing to tissue damage due to sustained immune activation. Understanding these interactions is important for developing therapeutic strategies targeting ULBP2-related pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds and activates the KLRK1/NKG2D receptor, mediating natural killer cell cytotoxicity.

See full target information ULBP2

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 16:597 PubMed40775202

2025

ULBP2 CAR-T cells enhance gastric cancer immunotherapy by inhibiting CAF activation.

Applications

Unspecified application

Species

Unspecified reactive species

Wentao Zhang,Wen Ren,Shuyan Guo,Haobo Han,Weiwen Cai,Haowen Bai,Long Li,Xiangyan Jiang,Xin Zheng,Tiansheng Zhang,Yan Wang,Huili Ye,Hongtai Cao,Wengui Shi,Huinian Zhou,Zeyuan Yu,Long Qin,Zuoyi Jiao

Frontiers in immunology 16:1515605 PubMed39963142

2025

Resveratrol contributes to NK cell-mediated breast cancer cytotoxicity by upregulating ULBP2 through miR-17-5p downmodulation and activation of MINK1/JNK/c-Jun signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Bisha Ding,Jie Li,Jia-Lin Yan,Chun-Yan Jiang,Ling-Bo Qian,Jie Pan

Oncology letters 29:15 PubMed39492940

2024

UL16‑binding protein 1 is a significant prognostic and diagnostic marker for breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaowei Zhang,Shuhong Dai,Liang Li,Pengyun Wang,Mingxin Dong

Heliyon 10:e34221 PubMed39082023

2024

T cell proliferation-related subtypes, prognosis model and characterization of tumor microenvironment in head and neck squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wanjin Jiang,Qi Yang,Xiaonan Yang,Ruijia Gan,Hongting Hua,Zhimin Ding,Dongyu Si,Xinbei Zhu,Xu Wang,Huabing Zhang,Chaobing Gao

Journal of ethnopharmacology 275:114175 PubMed33933571

2021

Jinfukang inhibits lung cancer metastasis by upregulating CX3CL1 to recruit NK cells to kill CTCs.

Applications

Unspecified application

Species

Unspecified reactive species

Zu-Jun Que,Jia-Liang Yao,Zhi-Yi Zhou,Pan Yu,Bin Luo,He-Gen Li,Jia-Xiang Liu,Hong-Xi Xu,Jian-Hui Tian

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ULBP2 antibody [EPR22303-124]

Immunoprecipitation - Anti-ULBP2 antibody [EPR22303-124] (AB275023)

Western blot - Anti-ULBP2 antibody [EPR22303-124] (AB275023)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise