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AB290746

Anti-ULBP2+ULBP5+ULBP6 antibody [EPR26171-156] (BSA and Azide free)

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Rabbit Recombinant Monoclonal ULBP6 antibody. Carrier free. Suitable for Flow Cyt, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

ULBP6, RAET1L, UL16-binding protein 6, Retinoic acid early transcript 1L protein

3 Images
Flow Cytometry (Intracellular) - Anti-ULBP2+ULBP5+ULBP6 antibody [EPR26171-156] (BSA and Azide free) (AB290746)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ULBP2+ULBP5+ULBP6 antibody [EPR26171-156] (BSA and Azide free) (AB290746)

This data was developed using ab290731, the same antibody clone in a different buffer formulation. Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized HEK-293T (Human embryonic kidney epithelial cell) transfected with human ULBP-5 (Left) and ULBP-6 (Right) expression vector cells labelling ULBP2+ULBP5+ULBP6 with ab290731 at 1/500 dilution (0.1ug)/ Left and Right (Red) compared with a isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Cells were surface stained with ab290731, then fixed with 2% PFA followed by intracellular staining with Myc-tag conjugated to Alexa Fluor® 647.

Flow Cytometry (Intracellular) - Anti-ULBP2+ULBP5+ULBP6 antibody [EPR26171-156] (BSA and Azide free) (AB290746)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ULBP2+ULBP5+ULBP6 antibody [EPR26171-156] (BSA and Azide free) (AB290746)

This data was developed using ab290731, the same antibody clone in a different buffer formulation. Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized HEK-293T (Human embryonic kidney epithelial cell) transfected with a human ULBP2 expression vector cells labelling ULBP2+ULBP5+ULBP6 with ab290731 at 1/500 dilution (0.1ug)/ Right (Red) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Cells were surface stained with rabbit IgG or ab290731, then fixed with 2% PFA followed by intracellular staining with Myc-tag conjugated to Alexa Fluor® 647.

Flow Cytometry - Anti-ULBP2+ULBP5+ULBP6 antibody [EPR26171-156] (BSA and Azide free) (AB290746)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-ULBP2+ULBP5+ULBP6 antibody [EPR26171-156] (BSA and Azide free) (AB290746)

This data was developed using ab290731, the same antibody clone in a different buffer formulation. Flow cytometric analysis of Raji (Human Burkitt's lymphoma B lymphocyte, Left) / BxPC-3 (Human pancreas adenocarcinoma epithelial cell, Right) cells labelling ULBP2+ULBP5+ULBP6 with ab290731 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Negative control : Raji.Gated on viable cells.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26171-156

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ULBP2 also known as UL16-binding protein 2 and N2DL2 is a glycoprotein with a mass of approximately 30 kDa. It belongs to the family of NKG2D ligands and plays an essential role in the immune response. This protein is expressed on the surface of a variety of cells including epithelial cells fibroblasts and activated immune cells. Its expression can be induced by cellular stress infection or transformation making it an important player in the regulation of the immune system.
Biological function summary

The function of ULBP2 is to facilitate the activation of natural killer (NK) cells and cytotoxic T lymphocytes through binding to the NKG2D receptor. ULBP2 is not a part of a complex but acts independently to trigger immune responses. By interacting with NKG2D ULBP2 promotes the release of cytokines and enhances the cytolytic activity of these immune cells leading to the destruction of infected or transformed cells. This mechanism allows the immune system to efficiently target and eliminate compromised cells.

Pathways

The interaction between ULBP2 and the NKG2D receptor forms a critical part of the immune surveillance pathway. This pathway detects and responds to cellular abnormalities such as tumorigenesis or viral infections. ULBP2's activity relates closely with other NKG2D ligands and components of the immune checkpoint pathways notably involving proteins like MICA and MICB. Additionally ULBP2’s interaction impacts the balance between activating and inhibitory signals within the NK cell cascade thereby influencing immune system homeostasis.

ULBP2 expression has significant implications in cancer and autoimmune conditions. Elevated levels of ULBP2 are frequently associated with various types of cancer where they can either stimulate anti-tumor immunity or be shed to evade immune detection. In this context the protein interacts with NKG2D which is also expressed on tumor-infiltrating lymphocytes. Additionally altered expression of ULBP2 has been observed in inflammatory disorders potentially contributing to tissue damage due to sustained immune activation. Understanding these interactions is important for developing therapeutic strategies targeting ULBP2-related pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds and activates the KLRK1/NKG2D receptor, mediating natural killer cell cytotoxicity.
See full target information RAET1L

Additional targets

ULBP2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com