Rabbit Recombinant Monoclonal ULBP3 antibody. Carrier free. Suitable for IP, Flow Cyt, Dot, WB and reacts with Human, Recombinant full length protein - Human samples.
Images
![Dot Blot - Anti-ULBP3 antibody [EPR22321-99] (BSA and Azide free) (AB300103), expandable thumbnail](https://content.abcam.com/products/images/ulbp3-antibody-epr22321-99-bsa-and-azide-free-ab300103--dot-blot-img400593.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-ULBP3 antibody [EPR22321-99] (BSA and Azide free) (AB300103)")
![Immunoprecipitation - Anti-ULBP3 antibody [EPR22321-99] (BSA and Azide free) (AB300103), expandable thumbnail](https://content.abcam.com/products/images/ulbp3-antibody-epr22321-99-bsa-and-azide-free-ab300103--immunoprecipitation-img400569.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-ULBP3 antibody [EPR22321-99] (BSA and Azide free) (AB300103)")
![Flow Cytometry (Intracellular) - Anti-ULBP3 antibody [EPR22321-99] (BSA and Azide free) (AB300103), expandable thumbnail](https://content.abcam.com/products/images/ulbp3-antibody-epr22321-99-bsa-and-azide-free-ab300103--flow-cytometry-intracellular-img400542.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-ULBP3 antibody [EPR22321-99] (BSA and Azide free) (AB300103)")
![Flow Cytometry (Intracellular) - Anti-ULBP3 antibody [EPR22321-99] (BSA and Azide free) (AB300103), expandable thumbnail](https://content.abcam.com/products/images/ulbp3-antibody-epr22321-99-bsa-and-azide-free-ab300103--flow-cytometry-intracellular-img400541.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-ULBP3 antibody [EPR22321-99] (BSA and Azide free) (AB300103)")
![Western blot - Anti-ULBP3 antibody [EPR22321-99] (BSA and Azide free) (AB300103), expandable thumbnail](https://content.abcam.com/products/images/ulbp3-antibody-epr22321-99-bsa-and-azide-free-ab300103--western-blot-img400455.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ULBP3 antibody [EPR22321-99] (BSA and Azide free) (AB300103)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | ICC/IF | IHC-P | Dot | WB | |
|---|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Not recommended | Expected | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Recombinant full length protein - Human | Not recommended | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Recombinant full length protein - Human | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Recombinant full length protein - Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Mouse, Recombinant full length protein - Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human, Recombinant full length protein - Human | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Recombinant full length protein - Human | Dilution info - | Notes - |
Target data
Function
Binds and activates the KLRK1/NKG2D receptor, mediating natural killer cell cytotoxicity.
Alternative names
N2DL3, RAET1N, ULBP3, UL16-binding protein 3, ALCAN-gamma, NKG2D ligand 3, Retinoic acid early transcript 1N, N2DL-3, NKG2DL3
Recommended products
Rabbit Recombinant Monoclonal ULBP3 antibody. Carrier free. Suitable for IP, Flow Cyt, Dot, WB and reacts with Human, Recombinant full length protein - Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR22321-99
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ULBP3 also known as UL16 binding protein 3 plays a significant role in immune surveillance. The protein is a member of the NKG2D ligand family and has a mass of approximately 30 kDa. ULBP3 is expressed on the cell surface of many human tissues predominantly in stressed transformed or infected cells where it becomes more prominent. The expression of ULBP3 signals to immune cells especially natural killer (NK) cells and some subsets of T-cells acting as an essential point of recognition and interaction.
- Biological function summary
ULBP3 interacts with the activating receptor NKG2D to initiate immune responses against tumor cells or pathogen-infected cells. The binding of ULBP3 to NKG2D forms a part of the recognition process leading to the activation of NK cells and cytotoxic T cells enhancing their ability to destroy target cells. ULBP3 operates independently and not as part of a larger ligand complex making its interactions directly important for regulating immune function across different environments and conditions aiding in the maintenance of cellular homeostasis.
- Pathways
ULBP3 participates integrally in immune signaling pathways like the NKG2D receptor-mediated pathway. Within this pathway its role in signaling enhances NK cell cytotoxicity and cytokine production. ULBP3 is related to other NKG2D ligands like MICA MICB and other ULBP molecules through this immune recognition pathway collectively contributing to immune defense mechanisms. These pathways form a critical element of the broader immune surveillance mechanism in the human body.
- Associated diseases and disorders
ULBP3 has associations with various cancers and infectious diseases. Overexpression of ULBP3 has been observed in certain types of cancers such as lymphoma where it promotes recognition by the NK cells. Another area of interest is its role in viral infections where pathogens may modulate ULBP3 expression to evade the immune system. ULBP3's interaction with proteins like NKG2D is vital in modulating immune responses during these diseases highlighting its potential both as a target for therapeutic intervention and a biomarker for disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Dot Blot - Anti-ULBP3 antibody [EPR22321-99] (BSA and Azide free) (ab300103), expandable thumbnail](https://content.abcam.com/products/images/ulbp3-antibody-epr22321-99-bsa-and-azide-free-ab300103--dot-blot-img400593.jpg "Dot Blot - Anti-ULBP3 antibody [EPR22321-99] (BSA and Azide free) (ab300103)")
Dot Blot - Anti-ULBP3 antibody [EPR22321-99] (BSA and Azide free) (ab300103)
This data was developed using Anti-ULBP3 antibody [EPR22321-99] ab300102, the same antibody clone in a different buffer formulation.
Dot blot analysis of ULBP3 using Anti-ULBP3 antibody [EPR22321-99] ab300102 at 1:1000 followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.
Lane 1: Human ULBP3 recombinant protein
Lane 2: Human ULBP1 recombinant protein
Lane 3: Human ULBP2 recombinant protein
Lane 4: Human ULBP5 recombinant protein
Lane 5: Human ULBP6 recombinant protein
Exposure time: 3 minutes
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
No cross-reactivity with other family members.
![Immunoprecipitation - Anti-ULBP3 antibody [EPR22321-99] (BSA and Azide free) (ab300103), expandable thumbnail](https://content.abcam.com/products/images/ulbp3-antibody-epr22321-99-bsa-and-azide-free-ab300103--immunoprecipitation-img400569.jpg "Immunoprecipitation - Anti-ULBP3 antibody [EPR22321-99] (BSA and Azide free) (ab300103)")
Immunoprecipitation - Anti-ULBP3 antibody [EPR22321-99] (BSA and Azide free) (ab300103)
This data was developed using Anti-ULBP3 antibody [EPR22321-99] ab300102, the same antibody clone in a different buffer formulation.ULBP3 was immunoprecipitated from 0.35 mg MDA-MB-231 (human breast adenocarcinoma epithelial cell) whole cell lysate 10 ug with Anti-ULBP3 antibody [EPR22321-99] ab300102 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-ULBP3 antibody [EPR22321-99] ab300102 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: MDA-MB-231 (human breast adenocarcinoma epithelial cell) whole cell lysate 10 ug
Lane 2: abAB300102 IP in MDA-MB-231 whole cell lysate
Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-ULBP3 antibody [EPR22321-99] ab300102 in MDA-MB-231 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 119 secondsAll lanes: Immunoprecipitation - Anti-ULBP3 antibody [EPR22321-99] (Anti-ULBP3 antibody [EPR22321-99] ab300102) at 1/1000 dilution
All lanes: MDA-MB-231 whole cell lysate at 10 µg
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 119s
![Flow Cytometry (Intracellular) - Anti-ULBP3 antibody [EPR22321-99] (BSA and Azide free) (ab300103), expandable thumbnail](https://content.abcam.com/products/images/ulbp3-antibody-epr22321-99-bsa-and-azide-free-ab300103--flow-cytometry-intracellular-img400542.jpg "Flow Cytometry (Intracellular) - Anti-ULBP3 antibody [EPR22321-99] (BSA and Azide free) (ab300103)")
Flow Cytometry (Intracellular) - Anti-ULBP3 antibody [EPR22321-99] (BSA and Azide free) (ab300103)
Flow cytometric analysis of Raji (human burkitt's lymphoma b lymphocyte, Left) / NAMALWA (human burkitt's lymphoma b lymphocyte, Right) cells labelling ULBP3 with Anti-ULBP3 antibody [EPR22321-99] ab300102 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti-Rabbit IgG (Alexa Fluor® 647, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed ab150083) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.Low expression: Raji(PMID: 12414645, 11827464).
![Flow Cytometry (Intracellular) - Anti-ULBP3 antibody [EPR22321-99] (BSA and Azide free) (ab300103), expandable thumbnail](https://content.abcam.com/products/images/ulbp3-antibody-epr22321-99-bsa-and-azide-free-ab300103--flow-cytometry-intracellular-img400541.jpg "Flow Cytometry (Intracellular) - Anti-ULBP3 antibody [EPR22321-99] (BSA and Azide free) (ab300103)")
Flow Cytometry (Intracellular) - Anti-ULBP3 antibody [EPR22321-99] (BSA and Azide free) (ab300103)
Flow cytometric analysis of MCF7 (human breast adenocarcinoma epithelial cell, Left) / HeLa (human cervix adenocarcinoma epithelial cell, Right) cells labelling ULBP3 with Anti-ULBP3 antibody [EPR22321-99] ab300102 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti-Rabbit IgG (Alexa Fluor® 647, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed ab150083) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.Low expression: MCF7(PMID: 25217158).
![Western blot - Anti-ULBP3 antibody [EPR22321-99] (BSA and Azide free) (ab300103), expandable thumbnail](https://content.abcam.com/products/images/ulbp3-antibody-epr22321-99-bsa-and-azide-free-ab300103--western-blot-img400455.jpg "Western blot - Anti-ULBP3 antibody [EPR22321-99] (BSA and Azide free) (ab300103)")
Western blot - Anti-ULBP3 antibody [EPR22321-99] (BSA and Azide free) (ab300103)
This data was developed using Anti-ULBP3 antibody [EPR22321-99] ab300102, the same antibody clone in a different buffer formulation. Blocking Buffer and concentration: 5% NFDM/TBST
All lanes: Western blot - Anti-ULBP3 antibody [EPR22321-99] (Anti-ULBP3 antibody [EPR22321-99] ab300102) at 1/1000 dilution
All lanes: Human testis tissue lysate
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Performed under reducing conditions.
Observed band size: 30 kDa
Exposure time: 3min
![Western blot - Anti-ULBP3 antibody [EPR22321-99] (BSA and Azide free) (ab300103), expandable thumbnail](https://content.abcam.com/products/images/ulbp3-antibody-epr22321-99-bsa-and-azide-free-ab300103--western-blot-img400454.jpg "Western blot - Anti-ULBP3 antibody [EPR22321-99] (BSA and Azide free) (ab300103)")
Western blot - Anti-ULBP3 antibody [EPR22321-99] (BSA and Azide free) (ab300103)
This data was developed using Anti-ULBP3 antibody [EPR22321-99] ab300102, the same antibody clone in a different buffer formulation. Blocking Buffer and concentration: 5% NFDM/TBST
All lanes: Western blot - Anti-ULBP3 antibody [EPR22321-99] (Anti-ULBP3 antibody [EPR22321-99] ab300102) at 1/1000 dilution
Lane 1: NAMALWA (human burkitts lymphoma b lymphocyte) whole cell lysate
Lane 2: Raji (human burkitts lymphoma b lymphocyte) whole cell lysate
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 30 kDa
Exposure time: 3min
![Western blot - Anti-ULBP3 antibody [EPR22321-99] (BSA and Azide free) (ab300103), expandable thumbnail](https://content.abcam.com/products/images/ulbp3-antibody-epr22321-99-bsa-and-azide-free-ab300103--western-blot-img400453.jpg "Western blot - Anti-ULBP3 antibody [EPR22321-99] (BSA and Azide free) (ab300103)")
Western blot - Anti-ULBP3 antibody [EPR22321-99] (BSA and Azide free) (ab300103)
This data was developed using Anti-ULBP3 antibody [EPR22321-99] ab300102, the same antibody clone in a different buffer formulation. Blocking Buffer and concentration: 5% NFDM/TBST
All lanes: Western blot - Anti-ULBP3 antibody [EPR22321-99] (Anti-ULBP3 antibody [EPR22321-99] ab300102) at 1/1000 dilution
Lane 1: SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 2: MDA-MB-231 (human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 3: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 4: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 9: PANC-1 (human pancreatic epithelioid carcinoma epithelial cell), whole cell lysate
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Performed under reducing conditions.
Observed band size: 30 kDa
Exposure time: 3min
![Western blot - Anti-ULBP3 antibody [EPR22321-99] (BSA and Azide free) (ab300103), expandable thumbnail](https://content.abcam.com/products/images/ulbp3-antibody-epr22321-99-bsa-and-azide-free-ab300103--western-blot-img400454.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ULBP3 antibody [EPR22321-99] (BSA and Azide free) (ab300103)")
![Western blot - Anti-ULBP3 antibody [EPR22321-99] (BSA and Azide free) (ab300103), expandable thumbnail](https://content.abcam.com/products/images/ulbp3-antibody-epr22321-99-bsa-and-azide-free-ab300103--western-blot-img400453.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ULBP3 antibody [EPR22321-99] (BSA and Azide free) (ab300103)")
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