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AB313499

Anti-ULBP3 antibody [RP23040254]

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Anti-Anti antibody [RP23040254] is a Rabbit Recombinant Multiclonal antibody that is used in Anti ICC/IF. Suitable for Human samples.

ULBP3 is a ligand for the NKG2D receptor, playing a crucial role in immune surveillance and tumor immunity. Research has shown that ULBP3 expression on tumor cells can enhance natural killer (NK) cell-mediated cytotoxicity, making it a promising target for cancer immunotherapy. Its ability to stimulate antitumor responses highlights its potential in developing novel cancer treatments.

View Alternative Names

N2DL3, RAET1N, ULBP3, UL16-binding protein 3, ALCAN-gamma, NKG2D ligand 3, Retinoic acid early transcript 1N, N2DL-3, NKG2DL3

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-ULBP3 Antibody [RP23040254] (AB313499)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ULBP3 Antibody [RP23040254] (AB313499)

Immunofluorescent analysis of GTL-16 cells fixed with 4% formaldehyde reconstituted in 1X PBS for 10 min at room temperature and permabilized using 0.1 % Triton X-100 in PBS for 15 min at room temperatulabeling endogenous ULBP3 with ab313499 at 1/100 dilution followed by Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 488 conjugate at 1/2000 dilution. Nuclei (blue) were stained using DAPI and Rhodamine Phalloidin at 1/300 dilution was used for cytoskeletal F-actin (red) staining. Panel a-d) clearly demonstrates membrane localization of ULBP3 in GTL-16 cells. Panel e-h) shows no specific staining in A549 cells which is ULBP3 negative, demonstrating specificity. The images were captured at 60X magnification.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ULBP3.

Q9BZM4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" } } }
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Product details

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.09% Sodium azide Constituents: 99.91% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ULBP3 also known as UL16 binding protein 3 plays a significant role in immune surveillance. The protein is a member of the NKG2D ligand family and has a mass of approximately 30 kDa. ULBP3 is expressed on the cell surface of many human tissues predominantly in stressed transformed or infected cells where it becomes more prominent. The expression of ULBP3 signals to immune cells especially natural killer (NK) cells and some subsets of T-cells acting as an essential point of recognition and interaction.
Biological function summary

ULBP3 interacts with the activating receptor NKG2D to initiate immune responses against tumor cells or pathogen-infected cells. The binding of ULBP3 to NKG2D forms a part of the recognition process leading to the activation of NK cells and cytotoxic T cells enhancing their ability to destroy target cells. ULBP3 operates independently and not as part of a larger ligand complex making its interactions directly important for regulating immune function across different environments and conditions aiding in the maintenance of cellular homeostasis.

Pathways

ULBP3 participates integrally in immune signaling pathways like the NKG2D receptor-mediated pathway. Within this pathway its role in signaling enhances NK cell cytotoxicity and cytokine production. ULBP3 is related to other NKG2D ligands like MICA MICB and other ULBP molecules through this immune recognition pathway collectively contributing to immune defense mechanisms. These pathways form a critical element of the broader immune surveillance mechanism in the human body.

ULBP3 has associations with various cancers and infectious diseases. Overexpression of ULBP3 has been observed in certain types of cancers such as lymphoma where it promotes recognition by the NK cells. Another area of interest is its role in viral infections where pathogens may modulate ULBP3 expression to evade the immune system. ULBP3's interaction with proteins like NKG2D is vital in modulating immune responses during these diseases highlighting its potential both as a target for therapeutic intervention and a biomarker for disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds and activates the KLRK1/NKG2D receptor, mediating natural killer cell cytotoxicity.
See full target information ULBP3
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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