AB177472

Anti-ULK1 antibody [EPR6153(2)]

20ul selling size
RabMAb
Recombinant
What is this?

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(5 Publications)

Rabbit Recombinant Monoclonal ULK1 antibody. Suitable for WB and reacts with Human samples. Cited in 5 publications.

View Alternative Names

KIAA0722, ULK1, Serine/threonine-protein kinase ULK1, Autophagy-related protein 1 homolog, Unc-51-like kinase 1, ATG1, hATG1

2 Images

Lab

Western blot - Anti-ULK1 antibody [EPR6153(2)] (AB177472)

Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ULK1 antibody [EPR6153(2)] (ab177472) at 1/20000 dilution

All lanes:

HEK293T (human embryonic kidney epithelial cell) transfected with human ULK1 expression vector, whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 112 kDa

Observed band size: 140 kDa

false

Exposure time: 30s

Supplier Data

Western blot - Anti-ULK1 antibody [EPR6153(2)] (AB177472)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The identity of the lower MW band at approximately 75 kDa (in lane 2) is unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-ULK1 antibody [EPR6153 (2)] (ab177472) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-ULK1 (phospho S467+S556+S638) antibody [RM1162] (<a href='/en-us/products/primary-antibodies/ulk1-phospho-s467s556s638-antibody-rm1162-ab322403'>ab322403</a>) at 1/1000 dilution

Lane 1:

HEK-293T (human embryonic kidney epithelial cell) transfected with an empty vector containing a His-tag, whole cell lysate (untreated membrane) at 20 µg

Lane 2:

HEK-293T transfected with a human ULK1 expression vector containing a His-tag, whole cell lysate (untreated membrane) at 20 µg

Lane 3:

HEK-293T transfected with a human ULK1 (S467A, S556A, S638A) expression vector containing a His-tag, whole cell lysate (untreated membrane) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 150 kDa,36 kDa

false

Exposure time: 180s

Carrier free

Anti-ULK1 antibody [EPR6153(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6153(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ULK1 also known as Unc-51-like kinase 1 is a serine/threonine-protein kinase with a molecular mass of approximately 112 kDa. ULK1 is expressed in many tissues including the brain heart and skeletal muscle and plays a significant mechanical role in initializing autophagy—a process vital for cellular recycling and homeostasis. The protein interacts with several autophagy-related proteins to facilitate its functions.

Biological function summary

This protein serves as an essential part of the ULK1 complex which includes ATG13 FIP200 and ATG101. This complex acts as an important regulator in the initiation stage of autophagy responding to cellular nutrient status and energy levels. ULK1 activity promotes the formation of the phagophore which is important for capturing and degrading cytoplasmic content.

Pathways

ULK1 plays a major role in autophagy and mTOR signaling pathways. The mTOR complex 1 (mTORC1) inhibits ULK1 activity under nutrient-rich conditions preventing unnecessary autophagy. When nutrients are scarce mTORC1 inhibition releases ULK1 enhancing autophagy initiation. ULK1 also interacts with AMPK an energy-sensing kinase adjusting cellular processes according to energy levels and promoting autophagy when necessary.

Research links ULK1 to cancer and neurodegenerative diseases. Deregulation of autophagy pathways influenced by impaired ULK1 activity associates with cancer progression and tumor growth. Cancer cells often exploit autophagy to survive in nutrient-poor environments. In neurodegenerative diseases such as Alzheimer's ULK1-mediated autophagy activity can help clear protein aggregates. Understanding ULK1 interactions with proteins like AMPK in these contexts could offer advancements in therapeutic approaches.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Serine/threonine-protein kinase involved in autophagy in response to starvation (PubMed : 18936157, PubMed : 21460634, PubMed : 21795849, PubMed : 23524951, PubMed : 25040165, PubMed : 29487085, PubMed : 31123703). Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes (PubMed : 18936157, PubMed : 21460634, PubMed : 21795849, PubMed : 25040165, PubMed : 39384743). Part of regulatory feedback loops in autophagy : acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR (PubMed : 21795849). Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity (PubMed : 21460634). May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences (PubMed : 18936157). Plays a role early in neuronal differentiation and is required for granule cell axon formation (PubMed : 11146101). Also phosphorylates SESN2 and SQSTM1 to regulate autophagy (PubMed : 25040165, PubMed : 37306101). Phosphorylates FLCN, promoting autophagy (PubMed : 25126726). Phosphorylates AMBRA1 in response to autophagy induction, releasing AMBRA1 from the cytoskeletal docking site to induce autophagosome nucleation (PubMed : 20921139). Phosphorylates ATG4B, leading to inhibit autophagy by decreasing both proteolytic activation and delipidation activities of ATG4B (PubMed : 28821708).

See full target information ULK1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cancer reports (Hoboken, N.J.) 7:e2128 PubMed39229655

2024

CircFAM188A Regulates Autophagy via miR-670-3p and ULK1 in Epithelial Ovarian Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Min Yong,Yuhua Zeng,Yuqin Yao,Miyuan Yang,Furong Tang,Hongtao Zhu,Jianguo Hu

BMC cardiovascular disorders 24:415 PubMed39123142

2024

Isoliquiritigenin attenuates myocardial ischemia reperfusion through autophagy activation mediated by AMPK/mTOR/ULK1 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Liying Shen,Yingwei Zhu,Zhenfeng Chen,Feng Shen,Weiwei Yu,Li Zhang

Translational oncology 46:101996 PubMed38795560

2024

Regulatory feedback loop between circ-EIF4A3 and EIF4A3 Enhances autophagy and growth in colorectal cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qingke Li,Zhiwu Wang,Jian Wang,Jiangong Wang,Xuan Zheng,Dan Li,Zhuo Wang,Jingwu Li,Yufeng Li

Nature communications 15:4195 PubMed38760351

2024

Ferritinophagy mediates adaptive resistance to EGFR tyrosine kinase inhibitors in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Wang,Qianfan Hu,Yuzhong Chen,Xing Huang,Yipeng Feng,Yuanjian Shi,Rutao Li,Xuewen Yin,Xuming Song,Yingkuan Liang,Te Zhang,Lin Xu,Gaochao Dong,Feng Jiang

Allergologia et immunopathologia 52:3-9 PubMed38459884

2024

RNF135 promotes cell proliferation and autophagy in lung adenocarcinoma by promoting the phosphorylation of ULK1.

Applications

Unspecified application

Species

Unspecified reactive species

Lichun Zhuang,Guanhui Shi,Yuejun Sun

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ULK1 antibody [EPR6153(2)]

Western blot - Anti-ULK1 antibody [EPR6153(2)] (AB177472)

Western blot - Anti-ULK1 antibody [EPR6153(2)] (AB177472)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise