Skip to main content

Rabbit Recombinant Monoclonal ULK1 phospho S450 antibody. Carrier free. Suitable for WB and reacts with Human samples.

Be the first to review this product! Submit a review

Images

Western blot - Anti-ULK1 (phospho S450) antibody [EPR6152] - BSA and Azide free (AB249223), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Not recommended
Tested

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Target data

Function

Serine/threonine-protein kinase involved in autophagy in response to starvation (PubMed:18936157, PubMed:21460634, PubMed:21795849, PubMed:23524951, PubMed:25040165, PubMed:29487085, PubMed:31123703). Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes (PubMed:18936157, PubMed:21460634, PubMed:21795849, PubMed:25040165). Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR (PubMed:21795849). Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity (PubMed:21460634). May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences (PubMed:18936157). Plays a role early in neuronal differentiation and is required for granule cell axon formation (PubMed:11146101). Also phosphorylates SESN2 and SQSTM1 to regulate autophagy (PubMed:25040165, PubMed:37306101). Phosphorylates FLCN, promoting autophagy (PubMed:25126726). Phosphorylates AMBRA1 in response to autophagy induction, releasing AMBRA1 from the cytoskeletal docking site to induce autophagosome nucleation (PubMed:20921139). Phosphorylates ATG4B, leading to inhibit autophagy by decreasing both proteolytic activation and delipidation activities of ATG4B (PubMed:28821708).

Alternative names

KIAA0722, ULK1, Serine/threonine-protein kinase ULK1, Autophagy-related protein 1 homolog, Unc-51-like kinase 1, ATG1, hATG1

Recommended products

Rabbit Recombinant Monoclonal ULK1 phospho S450 antibody. Carrier free. Suitable for WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR6152
Purification technique
Affinity purification
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab249223 is the carrier-free version of Anti-ULK1 (phospho S450) antibody [EPR6152] ab155971.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

ULK1 also known as Unc-51-like kinase 1 is a serine/threonine-protein kinase with a molecular mass of approximately 112 kDa. ULK1 is expressed in many tissues including the brain heart and skeletal muscle and plays a significant mechanical role in initializing autophagy—a process vital for cellular recycling and homeostasis. The protein interacts with several autophagy-related proteins to facilitate its functions.

Biological function summary

This protein serves as an essential part of the ULK1 complex which includes ATG13 FIP200 and ATG101. This complex acts as an important regulator in the initiation stage of autophagy responding to cellular nutrient status and energy levels. ULK1 activity promotes the formation of the phagophore which is important for capturing and degrading cytoplasmic content.

Pathways

ULK1 plays a major role in autophagy and mTOR signaling pathways. The mTOR complex 1 (mTORC1) inhibits ULK1 activity under nutrient-rich conditions preventing unnecessary autophagy. When nutrients are scarce mTORC1 inhibition releases ULK1 enhancing autophagy initiation. ULK1 also interacts with AMPK an energy-sensing kinase adjusting cellular processes according to energy levels and promoting autophagy when necessary.

Associated diseases and disorders

Research links ULK1 to cancer and neurodegenerative diseases. Deregulation of autophagy pathways influenced by impaired ULK1 activity associates with cancer progression and tumor growth. Cancer cells often exploit autophagy to survive in nutrient-poor environments. In neurodegenerative diseases such as Alzheimer's ULK1-mediated autophagy activity can help clear protein aggregates. Understanding ULK1 interactions with proteins like AMPK in these contexts could offer advancements in therapeutic approaches.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Western blot - Anti-ULK1 (phospho S450) antibody [EPR6152] - BSA and Azide free (ab249223), expandable thumbnail

    Western blot - Anti-ULK1 (phospho S450) antibody [EPR6152] - BSA and Azide free (ab249223)

    This data was developed using Anti-ULK1 (phospho S450) antibody [EPR6152] ab155971, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-ULK1 (phospho S450) antibody [EPR6152] (Anti-ULK1 (phospho S450) antibody [EPR6152] ab155971) at 1/1000 dilution

    Lane 1: 293T cell lysate transfected with ULK1. at 10 µg

    Lane 2: 293T cell lysate transfected with ULK1, treated with Lambda Phosphatase at 10 µg

    Predicted band size: 112 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com