Rabbit Recombinant Multiclonal ULK1 phospho S467 + S556 + S638 antibody. Suitable for WB, Dot and reacts with Transfected cell lysate - Human, Synthetic peptide samples.
Images
![Dot Blot - Anti-ULK1 (phospho S467+S556+S638) antibody [RM1162] (AB322403), expandable thumbnail](https://content.abcam.com/products/images/ulk1-phospho-s467s556s638-antibody-rm1162-ab322403--dot-blot-img439625.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-ULK1 (phospho S467+S556+S638) antibody [RM1162] (AB322403)")
![Western blot - Anti-ULK1 (phospho S467+S556+S638) antibody [RM1162] (AB322403), expandable thumbnail](https://content.abcam.com/products/images/ulk1-phospho-s467s556s638-antibody-rm1162-ab322403--western-blot-img439576.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ULK1 (phospho S467+S556+S638) antibody [RM1162] (AB322403)")
![Western blot - Anti-ULK1 (phospho S467+S556+S638) antibody [RM1162] (AB322403), expandable thumbnail](https://content.abcam.com/products/images/ulk1-phospho-s467s556s638-antibody-rm1162-ab322403--western-blot-img439575.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ULK1 (phospho S467+S556+S638) antibody [RM1162] (AB322403)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Multiclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | Flow Cyt (Intra) | Dot | |
|---|---|---|---|---|---|
| Human | Predicted | Not recommended | Not recommended | Not recommended | Predicted |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Synthetic peptide | Not recommended | Not recommended | Not recommended | Not recommended | Tested |
| Transfected cell lysate - Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human | Dilution info 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Synthetic peptide | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Transfected cell lysate - Human, Synthetic peptide | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Transfected cell lysate - Human, Mouse, Rat, Synthetic peptide | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Transfected cell lysate - Human, Mouse, Rat, Synthetic peptide | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Serine/threonine-protein kinase involved in autophagy in response to starvation (PubMed:18936157, PubMed:21460634, PubMed:21795849, PubMed:23524951, PubMed:25040165, PubMed:29487085, PubMed:31123703). Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes (PubMed:18936157, PubMed:21460634, PubMed:21795849, PubMed:25040165). Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR (PubMed:21795849). Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity (PubMed:21460634). May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences (PubMed:18936157). Plays a role early in neuronal differentiation and is required for granule cell axon formation (PubMed:11146101). Also phosphorylates SESN2 and SQSTM1 to regulate autophagy (PubMed:25040165, PubMed:37306101). Phosphorylates FLCN, promoting autophagy (PubMed:25126726). Phosphorylates AMBRA1 in response to autophagy induction, releasing AMBRA1 from the cytoskeletal docking site to induce autophagosome nucleation (PubMed:20921139). Phosphorylates ATG4B, leading to inhibit autophagy by decreasing both proteolytic activation and delipidation activities of ATG4B (PubMed:28821708).
Alternative names
KIAA0722, Serine/threonine-protein kinase ULK1, Autophagy-related protein 1 homolog, Unc-51-like kinase 1, ATG1, hATG1
Rabbit Recombinant Multiclonal ULK1 phospho S467 + S556 + S638 antibody. Suitable for WB, Dot and reacts with Transfected cell lysate - Human, Synthetic peptide samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Multiclonal
- Immunogens
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- RM1162
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:
- - The sensitivity of polyclonal antibodies by recognising multiple epitopes
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
View our range of recombinant multiclonal antibodies.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Dot Blot - Anti-ULK1 (phospho S467+S556+S638) antibody [RM1162] (ab322403), expandable thumbnail](https://content.abcam.com/products/images/ulk1-phospho-s467s556s638-antibody-rm1162-ab322403--dot-blot-img439625.jpg "Dot Blot - Anti-ULK1 (phospho S467+S556+S638) antibody [RM1162] (ab322403)")
Dot Blot - Anti-ULK1 (phospho S467+S556+S638) antibody [RM1162] (ab322403)
Dot blot analysis of ULK1 (phospho S467+S556+S638) using ab322403 at 1:1000 dilution (0.507 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.
Lane1: Human ULK1 (phospho S467) peptide
Lane2: Human ULK1 peptide
Lane3: Human ULK1 (phospho S556) peptide
Lane4: Human ULK1 peptide
Lane5: Human ULK1 (phospho S638) peptide
Lane6: Human ULK1 peptideExposure time: 180 seconds.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
All lanes: Dot Blot - Anti-ULK1 (phospho S467+S556+S638) antibody [RM1162] (ab322403) at 1/1000 dilution
Lane 1: Human ULK1 (phospho S467) peptide
Lanes 2, 4 and 6: Human ULK1 peptide
Lane 3: Human ULK1 (phospho S556) peptide
Lane 5: Human ULK1 (phospho S638) peptide
SecondaryAll lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Exposure time: 180s
![Western blot - Anti-ULK1 (phospho S467+S556+S638) antibody [RM1162] (ab322403), expandable thumbnail](https://content.abcam.com/products/images/ulk1-phospho-s467s556s638-antibody-rm1162-ab322403--western-blot-img439576.jpg "Western blot - Anti-ULK1 (phospho S467+S556+S638) antibody [RM1162] (ab322403)")
Western blot - Anti-ULK1 (phospho S467+S556+S638) antibody [RM1162] (ab322403)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The identity of the lower MW band at approximately 75 kDa (in lane 2 and 3) is unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-ULK1 (phospho S467+S556+S638) antibody [RM1162] (ab322403) at 1/1000 dilution
Lane 1: Untreated HEK-293T (human embryonic kidney epithelial cell) transfected with an empty vector containing a His-tag, whole cell lysate at 40 µg
Lane 2: HEK-293T transfected with an empty vector containing a His-tag were treated with 2mM Metformin for 2 hours, whole cell lysate at 40 µg
Lane 3: Untreated HEK-293T transfected with a human ULK1 expression vector containing a His-tag, whole cell lysate at 40 µg
Lane 4: HEK-293T transfected with a human ULK1 expression vector containing a His-tag were treated with 2mM Metformin for 2 hours, whole cell lysate at 40 µg
Lane 5: Untreated HEK-293T transfected with a human ULK1 (S467A, S556A, S638A) expression vector containing a His-tag, whole cell lysate at 40 µg
Lane 6: HEK-293T transfected with a human ULK1 (S467A, S556A, S638A) expression vector containing a His-tag were treated with 2mM Metformin for 2 hours, whole cell lysate at 40 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 150 kDa, 36 kDa
Exposure time: 26s
![Western blot - Anti-ULK1 (phospho S467+S556+S638) antibody [RM1162] (ab322403), expandable thumbnail](https://content.abcam.com/products/images/ulk1-phospho-s467s556s638-antibody-rm1162-ab322403--western-blot-img439575.jpg "Western blot - Anti-ULK1 (phospho S467+S556+S638) antibody [RM1162] (ab322403)")
Western blot - Anti-ULK1 (phospho S467+S556+S638) antibody [RM1162] (ab322403)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The identity of the lower MW band at approximately 75 kDa (in lane 2) is unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
In Western blot, Anti-ULK1 antibody [EPR6153 (2)] (Anti-ULK1 antibody [EPR6153(2)] ab177472) staining at 1/1000 dilution.
All lanes: Western blot - Anti-ULK1 (phospho S467+S556+S638) antibody [RM1162] (ab322403) at 1/1000 dilution
Lane 1: HEK-293T (human embryonic kidney epithelial cell) transfected with an empty vector containing a His-tag, whole cell lysate (untreated membrane) at 20 µg
Lane 2: HEK-293T transfected with a human ULK1 expression vector containing a His-tag, whole cell lysate (untreated membrane) at 20 µg
Lane 3: HEK-293T transfected with a human ULK1 (S467A, S556A, S638A) expression vector containing a His-tag, whole cell lysate (untreated membrane) at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 150 kDa, 36 kDa
Exposure time: 180s
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