Rabbit Polyclonal ULK1 phospho S555 antibody. Suitable for WB and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ULK1 phospho S555.
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- The Journal of physiology 601:2917-29332023High-intensity interval training in the form of isometric contraction improves fatigue resistance in dystrophin-deficient muscle.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNao Yamauchi,Katsuyuki Tamai,Iori Kimura,Azuma Naito,Nao Tokuda,Yuki Ashida,Norio Motohashi,Yoshitsugu Aoki,Takashi YamadaPubMed 37184335
- Nature communications 13:11722022Oxygen-sensitive methylation of ULK1 is required for hypoxia-induced autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingyi Li,Tao Zhang,Tao Ren,Xiaoyu Liao,Yilong Hao,Je Sun Lim,Jong-Ho Lee,Mi Li,Jichun Shao,Rui LiuPubMed 35246531
- BMC cancer 21:1952021Blockage of AMPK-ULK1 pathway mediated autophagy promotes cell apoptosis to increase doxorubicin sensitivity in breast cancer (BC) cells: an in vitro study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLibo Yu,Qingtao Shi,Yan Jin,Zhixin Liu,Jiaxin Li,Wenzhou SunPubMed 33632157
- Molecular medicine reports 22:1335-13412020Diosmetin alleviates hypoxia‑induced myocardial apoptosis by inducing autophagy through AMPK activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQijun Si et. al.PubMed 32627001
- Nutrients 11:2019Exercise Mitigates the Loss of Muscle Mass by Attenuating the Activation of Autophagy during Severe Energy Deficit.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarcos Martin-Rincon et. al.PubMed 31752260
- Stem cells (Dayton, Ohio) 37:1542-15552019Mitochondrial Phosphoenolpyruvate Carboxykinase Regulates Osteogenic Differentiation by Modulating AMPK/ULK1-Dependent Autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZheng Li et. al.PubMed 31574189
- Pharmacological research 148:1044612019Enhancement of epithelial cell autophagy induced by sinensetin alleviates epithelial barrier dysfunction in colitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYong-Jian Xiong et. al.PubMed 31542404
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ULK1 phospho S555. The exact immunogen used to generate this antibody is proprietary information. Database link O75385
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
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7 products for Alternative Product
Target data
Function
Serine/threonine-protein kinase involved in autophagy in response to starvation (PubMed:18936157, PubMed:21460634, PubMed:21795849, PubMed:23524951, PubMed:25040165, PubMed:29487085, PubMed:31123703). Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes (PubMed:18936157, PubMed:21460634, PubMed:21795849, PubMed:25040165). Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR (PubMed:21795849). Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity (PubMed:21460634). May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences (PubMed:18936157). Plays a role early in neuronal differentiation and is required for granule cell axon formation (PubMed:11146101). Also phosphorylates SESN2 and SQSTM1 to regulate autophagy (PubMed:25040165, PubMed:37306101). Phosphorylates FLCN, promoting autophagy (PubMed:25126726). Phosphorylates AMBRA1 in response to autophagy induction, releasing AMBRA1 from the cytoskeletal docking site to induce autophagosome nucleation (PubMed:20921139). Phosphorylates ATG4B, leading to inhibit autophagy by decreasing both proteolytic activation and delipidation activities of ATG4B (PubMed:28821708).
Alternative names
KIAA0722, ULK1, Serine/threonine-protein kinase ULK1, Autophagy-related protein 1 homolog, Unc-51-like kinase 1, ATG1, hATG1
Recommended products
Rabbit Polyclonal ULK1 phospho S555 antibody. Suitable for WB and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ULK1 phospho S555.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ULK1 phospho S555. The exact immunogen used to generate this antibody is proprietary information. Database link O75385
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ULK1 also known as Unc-51-like kinase 1 is a serine/threonine-protein kinase with a molecular mass of approximately 112 kDa. ULK1 is expressed in many tissues including the brain heart and skeletal muscle and plays a significant mechanical role in initializing autophagy—a process vital for cellular recycling and homeostasis. The protein interacts with several autophagy-related proteins to facilitate its functions.
- Biological function summary
This protein serves as an essential part of the ULK1 complex which includes ATG13 FIP200 and ATG101. This complex acts as an important regulator in the initiation stage of autophagy responding to cellular nutrient status and energy levels. ULK1 activity promotes the formation of the phagophore which is important for capturing and degrading cytoplasmic content.
- Pathways
ULK1 plays a major role in autophagy and mTOR signaling pathways. The mTOR complex 1 (mTORC1) inhibits ULK1 activity under nutrient-rich conditions preventing unnecessary autophagy. When nutrients are scarce mTORC1 inhibition releases ULK1 enhancing autophagy initiation. ULK1 also interacts with AMPK an energy-sensing kinase adjusting cellular processes according to energy levels and promoting autophagy when necessary.
- Associated diseases and disorders
Research links ULK1 to cancer and neurodegenerative diseases. Deregulation of autophagy pathways influenced by impaired ULK1 activity associates with cancer progression and tumor growth. Cancer cells often exploit autophagy to survive in nutrient-poor environments. In neurodegenerative diseases such as Alzheimer's ULK1-mediated autophagy activity can help clear protein aggregates. Understanding ULK1 interactions with proteins like AMPK in these contexts could offer advancements in therapeutic approaches.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-ULK1 (phospho S555) antibody (ab229537)
7.5% SDS-PAGE gel.
All lanes: Western blot - Anti-ULK1 (phospho S555) antibody (ab229537) at 1/1000 dilution
Lane 1: Untreated MCF7 (human breast adenocarcinoma cell line) whole cell extract at 60 µg
Lane 2: MCF7, treated with 100 µM CCCP for 2 hours, whole cell extract at 60 µg
Predicted band size: 112 kDa
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