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AB133747

Anti-ULK1 (phospho S556) antibody [EPR6156]

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(14 Publications)

Rabbit Recombinant Monoclonal ULK1 phospho S556 antibody. Suitable for Dot, WB and reacts with Human samples. Cited in 14 publications.

View Alternative Names

KIAA0722, ULK1, Serine/threonine-protein kinase ULK1, Autophagy-related protein 1 homolog, Unc-51-like kinase 1, ATG1, hATG1

4 Images
Western blot - Anti-ULK1 (phospho S556) antibody [EPR6156] (AB133747)
  • WB

Unknown

Western blot - Anti-ULK1 (phospho S556) antibody [EPR6156] (AB133747)

The bottom panel shows total levels of ULK1 in both samples.

All lanes:

Western blot - Anti-ULK1 (phospho S556) antibody [EPR6156] (ab133747) at 1/10000 dilution

Lane 1:

ULK1 transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate untreated at 10 µg

Lane 2:

ULK1 transfected HEK-293T cell lysate treated with Lambda Phosphatase at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 112 kDa

Observed band size: 120 kDa

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Western blot - Anti-ULK1 (phospho S556) antibody [EPR6156] (AB133747)
  • WB

Lab

Western blot - Anti-ULK1 (phospho S556) antibody [EPR6156] (AB133747)

Blocking and diluting buffer used was 5% NFDM/TBST.

All lanes:

Western blot - Anti-ULK1 (phospho S556) antibody [EPR6156] (ab133747) at 1/5000 dilution

Lane 1:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates at 15 µg

Lane 2:

HEK-293 treated with epidermal growth factor. at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 112 kDa

Observed band size: 120 kDa

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Dot Blot - Anti-ULK1 (phospho S556) antibody [EPR6156] (AB133747)
  • Dot

Lab

Dot Blot - Anti-ULK1 (phospho S556) antibody [EPR6156] (AB133747)

All lanes : Anti-ULK1 (phospho S556) antibody [EPR6156] (ab133747) at 1/1000 dilution

Lane 1 : Human ULK1 (pS556) phospho peptide

Lane 2 : Human ULK1 non-phospho peptide

Secondary antibody : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size : 112 kDa

Exposure time : 3 minutes

Blocking and diluting buffer used was 5% NFDM/TBST.

OI-RD Scanning - Anti-ULK1 (phospho S556) antibody [EPR6156] (AB133747)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-ULK1 (phospho S556) antibody [EPR6156] (AB133747)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Carrier free

    Anti-ULK1 (phospho S556) antibody [EPR6156] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6156

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ULK1 also known as Unc-51-like kinase 1 is a serine/threonine-protein kinase with a molecular mass of approximately 112 kDa. ULK1 is expressed in many tissues including the brain heart and skeletal muscle and plays a significant mechanical role in initializing autophagy—a process vital for cellular recycling and homeostasis. The protein interacts with several autophagy-related proteins to facilitate its functions.
Biological function summary

This protein serves as an essential part of the ULK1 complex which includes ATG13 FIP200 and ATG101. This complex acts as an important regulator in the initiation stage of autophagy responding to cellular nutrient status and energy levels. ULK1 activity promotes the formation of the phagophore which is important for capturing and degrading cytoplasmic content.

Pathways

ULK1 plays a major role in autophagy and mTOR signaling pathways. The mTOR complex 1 (mTORC1) inhibits ULK1 activity under nutrient-rich conditions preventing unnecessary autophagy. When nutrients are scarce mTORC1 inhibition releases ULK1 enhancing autophagy initiation. ULK1 also interacts with AMPK an energy-sensing kinase adjusting cellular processes according to energy levels and promoting autophagy when necessary.

Research links ULK1 to cancer and neurodegenerative diseases. Deregulation of autophagy pathways influenced by impaired ULK1 activity associates with cancer progression and tumor growth. Cancer cells often exploit autophagy to survive in nutrient-poor environments. In neurodegenerative diseases such as Alzheimer's ULK1-mediated autophagy activity can help clear protein aggregates. Understanding ULK1 interactions with proteins like AMPK in these contexts could offer advancements in therapeutic approaches.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Serine/threonine-protein kinase involved in autophagy in response to starvation (PubMed : 18936157, PubMed : 21460634, PubMed : 21795849, PubMed : 23524951, PubMed : 25040165, PubMed : 29487085, PubMed : 31123703). Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes (PubMed : 18936157, PubMed : 21460634, PubMed : 21795849, PubMed : 25040165). Part of regulatory feedback loops in autophagy : acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR (PubMed : 21795849). Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity (PubMed : 21460634). May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences (PubMed : 18936157). Plays a role early in neuronal differentiation and is required for granule cell axon formation (PubMed : 11146101). Also phosphorylates SESN2 and SQSTM1 to regulate autophagy (PubMed : 25040165, PubMed : 37306101). Phosphorylates FLCN, promoting autophagy (PubMed : 25126726). Phosphorylates AMBRA1 in response to autophagy induction, releasing AMBRA1 from the cytoskeletal docking site to induce autophagosome nucleation (PubMed : 20921139). Phosphorylates ATG4B, leading to inhibit autophagy by decreasing both proteolytic activation and delipidation activities of ATG4B (PubMed : 28821708).
See full target information ULK1 phospho S556
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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Cancer science 115:3466-3480 PubMed39118482

2024

CLG promotes mTOR/ULK1 pathway-mediated autophagy to inhibit OS development by inhibiting TRAF6-mediated FLT3 ubiquitination.

Applications

Unspecified application

Species

Unspecified reactive species

Xiongjie Huang,Yanran Huang,Bin Peng,Junfang Wang,Huiyu Tang,Yanming Chen

iScience 27:109321 PubMed38558936

2024

AP39 through AMPK-ULK1-FUNDC1 pathway regulates mitophagy, inhibits pyroptosis, and improves doxorubicin-induced myocardial fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Junxiong Zhao,Ting Yang,Jiali Yi,Hongmin Hu,Qi Lai,Liangui Nie,Maojun Liu,Chun Chu,Jun Yang

Cancer letters 577:216422 PubMed37805162

2023

Rigosertib promotes anti-tumor immunity via autophagic degradation of PD-L1 in colorectal cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyi Zhou,Dongliang Fu,Hang Yang,Chenqin Le,Yier Lu,Jingsun Wei,Yang Tang,Jiawei Zhang,Ying Yuan,Kefeng Ding,Qian Xiao

Journal of diabetes investigation 14:1344-1355 PubMed37688345

2023

Exosomal circHIPK3 derived from umbilical cord-derived mesenchymal stem cells enhances skin fibroblast autophagy by blocking miR-20b-5p/ULK1/Atg13 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi-Yang Qiu,Shi-Shuai Lin,Nan-Fang Pan,Zhi-Hu Lin,Yun-Chuan Pan,Zun-Hong Liang

Cells 11: PubMed36552800

2022

FUT2 Facilitates Autophagy and Suppresses Apoptosis via p53 and JNK Signaling in Lung Adenocarcinoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yuqi Zhang,Enze Yao,Yijing Liu,Yining Zhang,Mengyang Ding,Jingyu Liu,Xiaoming Chen,Sairong Fan

Open life sciences 17:710-725 PubMed35859615

2022

MT-12 inhibits the proliferation of bladder cells and by enhancing autophagy through mitochondrial dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Chen,Chengxing Xia,Chunwei Ye,Feineng Liu,Yitian Ou,Ruping Yan,Haifeng Wang,Delin Yang

Metabolites 11: PubMed34940569

2021

AMPK-Mediated Metabolic Switching Is High Effective for Phytochemical Levo-Tetrahydropalmatine (l-THP) to Reduce Hepatocellular Carcinoma Tumor Growth.

Applications

Unspecified application

Species

Unspecified reactive species

Xunzhe Yin,Wenbo Li,Jiaxin Zhang,Wenjing Zhao,Huaxing Cai,Chi Zhang,Zuojia Liu,Yan Guo,Jin Wang

Frontiers in cellular neuroscience 14:555591 PubMed32982695

2020

Inhibition of Brd4 by JQ1 Promotes Functional Recovery From Spinal Cord Injury by Activating Autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Yao Li,Jie Xiang,Jing Zhang,Jiahao Lin,Yaosen Wu,Xiangyang Wang

Autophagy 16:1905-1914 PubMed31965901

2020

Endothelial autophagy deficiency induces IL6 - dependent endothelial mesenchymal transition and organ fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuta Takagaki,Seon Myeong Lee,Zha Dongqing,Munehiro Kitada,Keizo Kanasaki,Daisuke Koya

Cell reports 27:2075-2091.e5 PubMed31091447

2019

The Glycoprotein and Nucleocapsid Protein of Hantaviruses Manipulate Autophagy Flux to Restrain Host Innate Immune Responses.

Applications

Unspecified application

Species

Unspecified reactive species

Kerong Wang,Hongwei Ma,He Liu,Wei Ye,Zhuo Li,Linfeng Cheng,Liang Zhang,Yingfeng Lei,Lixin Shen,Fanglin Zhang
View all publications
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