Rabbit Recombinant Monoclonal ULK1 phospho S638 antibody. Carrier free. Suitable for WB and reacts with Human samples.
Images
![Western blot - Anti-ULK1 (phospho S638) antibody [EPR6155] - BSA and Azide free (AB248647), expandable thumbnail](https://content.abcam.com/products/images/ulk1-phospho-s638-antibody-epr6155-bsa-and-azide-free-ab248647--western-blot-img126965.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ULK1 (phospho S638) antibody [EPR6155] - BSA and Azide free (AB248647)")
![OI-RD Scanning - Anti-ULK1 (phospho S638) antibody [EPR6155] - BSA and Azide free (AB248647), expandable thumbnail](https://content.abcam.com/products/images/ulk1-phospho-s638-antibody-epr6155-bsa-and-azide-free-ab248647--oi-rd-scanning-img97633.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-ULK1 (phospho S638) antibody [EPR6155] - BSA and Azide free (AB248647)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | WB | IHC-P | |
|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Serine/threonine-protein kinase involved in autophagy in response to starvation (PubMed:18936157, PubMed:21460634, PubMed:21795849, PubMed:23524951, PubMed:25040165, PubMed:29487085, PubMed:31123703). Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes (PubMed:18936157, PubMed:21460634, PubMed:21795849, PubMed:25040165). Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR (PubMed:21795849). Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity (PubMed:21460634). May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences (PubMed:18936157). Plays a role early in neuronal differentiation and is required for granule cell axon formation (PubMed:11146101). Also phosphorylates SESN2 and SQSTM1 to regulate autophagy (PubMed:25040165, PubMed:37306101). Phosphorylates FLCN, promoting autophagy (PubMed:25126726). Phosphorylates AMBRA1 in response to autophagy induction, releasing AMBRA1 from the cytoskeletal docking site to induce autophagosome nucleation (PubMed:20921139). Phosphorylates ATG4B, leading to inhibit autophagy by decreasing both proteolytic activation and delipidation activities of ATG4B (PubMed:28821708).
Alternative names
KIAA0722, ULK1, Serine/threonine-protein kinase ULK1, Autophagy-related protein 1 homolog, Unc-51-like kinase 1, ATG1, hATG1
Recommended products
Rabbit Recombinant Monoclonal ULK1 phospho S638 antibody. Carrier free. Suitable for WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR6155
- Purification technique
- Affinity purification
- Dissociation constant
- 5.43 x 10-11 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab248647 is the carrier-free version of Anti-ULK1 (phospho S638) antibody [EPR6155] ab133766.
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ULK1 also known as Unc-51-like kinase 1 is a serine/threonine-protein kinase with a molecular mass of approximately 112 kDa. ULK1 is expressed in many tissues including the brain heart and skeletal muscle and plays a significant mechanical role in initializing autophagy—a process vital for cellular recycling and homeostasis. The protein interacts with several autophagy-related proteins to facilitate its functions.
- Biological function summary
This protein serves as an essential part of the ULK1 complex which includes ATG13 FIP200 and ATG101. This complex acts as an important regulator in the initiation stage of autophagy responding to cellular nutrient status and energy levels. ULK1 activity promotes the formation of the phagophore which is important for capturing and degrading cytoplasmic content.
- Pathways
ULK1 plays a major role in autophagy and mTOR signaling pathways. The mTOR complex 1 (mTORC1) inhibits ULK1 activity under nutrient-rich conditions preventing unnecessary autophagy. When nutrients are scarce mTORC1 inhibition releases ULK1 enhancing autophagy initiation. ULK1 also interacts with AMPK an energy-sensing kinase adjusting cellular processes according to energy levels and promoting autophagy when necessary.
- Associated diseases and disorders
Research links ULK1 to cancer and neurodegenerative diseases. Deregulation of autophagy pathways influenced by impaired ULK1 activity associates with cancer progression and tumor growth. Cancer cells often exploit autophagy to survive in nutrient-poor environments. In neurodegenerative diseases such as Alzheimer's ULK1-mediated autophagy activity can help clear protein aggregates. Understanding ULK1 interactions with proteins like AMPK in these contexts could offer advancements in therapeutic approaches.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-ULK1 (phospho S638) antibody [EPR6155] - BSA and Azide free (ab248647), expandable thumbnail](https://content.abcam.com/products/images/ulk1-phospho-s638-antibody-epr6155-bsa-and-azide-free-ab248647--western-blot-img126965.jpg "Western blot - Anti-ULK1 (phospho S638) antibody [EPR6155] - BSA and Azide free (ab248647)")
Western blot - Anti-ULK1 (phospho S638) antibody [EPR6155] - BSA and Azide free (ab248647)
This data was developed using Anti-ULK1 (phospho S638) antibody [EPR6155] ab133766, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-ULK1 (phospho S638) antibody [EPR6155] (Anti-ULK1 (phospho S638) antibody [EPR6155] ab133766) at 1/1000 dilution
Lane 1: ULK1 transfected 293T cell lysate at 10 µg
Lane 2: ULK1 transfected 293T cell lysate, treated with Lambda Phosphatase at 10 µg
SecondaryAll lanes: HRP labelled Goat anti Rabbit IgG at 1/2000 dilution
Predicted band size: 112 kDa
![OI-RD Scanning - Anti-ULK1 (phospho S638) antibody [EPR6155] - BSA and Azide free (ab248647), expandable thumbnail](https://content.abcam.com/products/images/ulk1-phospho-s638-antibody-epr6155-bsa-and-azide-free-ab248647--oi-rd-scanning-img97633.jpg "OI-RD Scanning - Anti-ULK1 (phospho S638) antibody [EPR6155] - BSA and Azide free (ab248647)")
OI-RD Scanning - Anti-ULK1 (phospho S638) antibody [EPR6155] - BSA and Azide free (ab248647)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
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Product protocols
- Visit the general protocols
- Visit troubleshooting
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