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AB229909

Anti-ULK1 (phospho S757) antibody [EPR22265-9]

3

(1 Review)

|

(11 Publications)

Rabbit Recombinant Monoclonal ULK1 phospho S757 antibody. Suitable for IP, Dot, WB and reacts with Mouse, Synthetic peptide samples. Cited in 11 publications.

View Alternative Names

Serine/threonine-protein kinase ULK1, Serine/threonine-protein kinase Unc51.1, Unc-51-like kinase 1, Ulk1

4 Images
Immunoprecipitation - Anti-ULK1 (phospho S757) antibody [EPR22265-9] (AB229909)
  • IP

Unknown

Immunoprecipitation - Anti-ULK1 (phospho S757) antibody [EPR22265-9] (AB229909)

ULK1 (phospho S757) was immunoprecipitated from 0.35 mg MEF (mouse embryonic fibroblast (immortalized)) whole cell lysate with ab229909 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab229909 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution. Lysate were made freshly and used in IP test immediately to minimize protein degradation. Incubation time was 2h.

Lane 1 : MEF (mouse embryonic fibroblast (immortalized)) whole cell lysate, 10μg.

Lane 2 : ab229909 IP in MEF whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab229909 in MEF whole cell lysate.

Blocking/dilution buffer : 5% NFDM/TBST.

Exposure time : 3 mins.

All lanes:

Immunoprecipitation - Anti-ULK1 (phospho S757) antibody [EPR22265-9] (ab229909)

Predicted band size: 112 kDa

Observed band size: 150 kDa

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Western blot - Anti-ULK1 (phospho S757) antibody [EPR22265-9] (AB229909)
  • WB

Lab

Western blot - Anti-ULK1 (phospho S757) antibody [EPR22265-9] (AB229909)

WB membrane in lane 2 was treated with alkaline phosphatase for 1h at 37°C. This blot was developed using a higher sensitivity ECL substrate.

Blocking/Dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 59 seconds.

All lanes:

Western blot - Anti-ULK1 (phospho S757) antibody [EPR22265-9] (ab229909) at 1/1000 dilution

Lane 1:

MEF (mouse embryonic fibroblast (immortalized)), whole cell lysate at 20 µg

Lane 2:

MEF whole cell lysate (Phosphatase treated membrane) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 112 kDa

Observed band size: 150 kDa

false

Western blot - Anti-ULK1 (phospho S757) antibody [EPR22265-9] (AB229909)
  • WB

Lab

Western blot - Anti-ULK1 (phospho S757) antibody [EPR22265-9] (AB229909)

Phosphorylation level of S757 is reduced during starvation (PMID : 28835610, 21258367). This blot was developed using a higher sensitivity ECL substrate.

Blocking/Diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : 59 seconds.

All lanes:

Western blot - Anti-ULK1 (phospho S757) antibody [EPR22265-9] (ab229909) at 1/1000 dilution

Lane 1:

Untreated MEF (mouse embryonic fibroblast (immortalized)), whole cell lysate at 20 µg

Lane 2:

MEF cells were cultured in amino acid and serum-free, glucose-containing starvation buffer (EBSS) for 16 hours, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 112 kDa

Observed band size: 150 kDa

false

Exposure time: 59s

Dot Blot - Anti-ULK1 (phospho S757) antibody [EPR22265-9] (AB229909)
  • Dot

Unknown

Dot Blot - Anti-ULK1 (phospho S757) antibody [EPR22265-9] (AB229909)

Dot blot analysis using ab229909 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100,000 diltution.

Lane 1 : ULK1 (phospho S757) peptide.

Lane 2 : ULK1 non-phospho peptide.

Blocking/diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22265-9

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

IP, WB, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Synthetic peptide": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ULK1 also known as Unc-51-like kinase 1 is a serine/threonine-protein kinase with a molecular mass of approximately 112 kDa. ULK1 is expressed in many tissues including the brain heart and skeletal muscle and plays a significant mechanical role in initializing autophagy—a process vital for cellular recycling and homeostasis. The protein interacts with several autophagy-related proteins to facilitate its functions.
Biological function summary

This protein serves as an essential part of the ULK1 complex which includes ATG13 FIP200 and ATG101. This complex acts as an important regulator in the initiation stage of autophagy responding to cellular nutrient status and energy levels. ULK1 activity promotes the formation of the phagophore which is important for capturing and degrading cytoplasmic content.

Pathways

ULK1 plays a major role in autophagy and mTOR signaling pathways. The mTOR complex 1 (mTORC1) inhibits ULK1 activity under nutrient-rich conditions preventing unnecessary autophagy. When nutrients are scarce mTORC1 inhibition releases ULK1 enhancing autophagy initiation. ULK1 also interacts with AMPK an energy-sensing kinase adjusting cellular processes according to energy levels and promoting autophagy when necessary.

Research links ULK1 to cancer and neurodegenerative diseases. Deregulation of autophagy pathways influenced by impaired ULK1 activity associates with cancer progression and tumor growth. Cancer cells often exploit autophagy to survive in nutrient-poor environments. In neurodegenerative diseases such as Alzheimer's ULK1-mediated autophagy activity can help clear protein aggregates. Understanding ULK1 interactions with proteins like AMPK in these contexts could offer advancements in therapeutic approaches.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Serine/threonine-protein kinase involved in autophagy in response to starvation (PubMed : 10624947, PubMed : 19258318, PubMed : 21205641, PubMed : 21258367, PubMed : 21460634, PubMed : 25040165, PubMed : 25723488). Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes (PubMed : 10624947, PubMed : 19258318, PubMed : 21205641, PubMed : 21258367, PubMed : 21460634, PubMed : 25040165). Part of regulatory feedback loops in autophagy : acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR (PubMed : 21205641, PubMed : 21258367). Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity (PubMed : 21460634). May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences (PubMed : 19258318). Plays a role early in neuronal differentiation and is required for granule cell axon formation (By similarity). Also phosphorylates SESN2 and SQSTM1 to regulate autophagy (PubMed : 25040165, PubMed : 25723488). Phosphorylates FLCN, promoting autophagy (By similarity). Phosphorylates AMBRA1 in response to autophagy induction, releasing AMBRA1 from the cytoskeletal docking site to induce autophagosome nucleation (By similarity). Phosphorylates ATG4B, leading to inhibit autophagy by decreasing both proteolytic activation and delipidation activities of ATG4B (By similarity).
See full target information Ulk1 phospho S757
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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Biomolecules 15: PubMed40867632

2025

Apelin-13-Mediated Upregulation of METTL3 Ameliorates Alzheimer's Disease via Inhibiting Neuroinflammation Through m6A-Dependent Regulation of lncRNA BDNF-AS.

Applications

Unspecified application

Species

Unspecified reactive species

Li Han,Siwen Wei,Rong Wang,Yiran Liu,Yi Zhong,Juan Fu,Huaiqing Luo,Meihua Bao

Frontiers in pharmacology 16:1550458 PubMed40599805

2025

Naringenin attenuates slow-transit constipation by regulating the AMPK/mTOR/ULK1 signalling pathway: and studies.

Applications

Unspecified application

Species

Unspecified reactive species

Yahui Wang,Xiaopeng Wang,Yifei Qian,Mingming Sun,Huiju Yang,Lianlin Su,Shuai Yan

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2404080 PubMed39041921

2024

PP2Ac Regulates Autophagy via Mediating mTORC1 and ULK1 During Osteoclastogenesis in the Subchondral Bone of Osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Haifeng Zhang,Gaoran Ge,Wei Zhang,Houyi Sun,Xiaolong Liang,Yu Xia,Jiacheng Du,Zerui Wu,Jiaxiang Bai,Huilin Yang,Xing Yang,Jun Zhou,Yaozeng Xu,Dechun Geng

Virulence 15:2327096 PubMed38466143

2024

The role of HDAC6 in enhancing macrophage autophagy via the autophagolysosomal pathway to alleviate legionella pneumophila-induced pneumonia.

Applications

Unspecified application

Species

Unspecified reactive species

Minjia Chen,Xiuqin Cao,Ronghui Zheng,Haixia Chen,Ruixia He,Hao Zhou,Zhiwei Yang

Redox biology 52:102288 PubMed35325804

2022

Empagliflozin attenuates cardiac microvascular ischemia/reperfusion through activating the AMPKα1/ULK1/FUNDC1/mitophagy pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Cai,Zhongzhou Guo,Xing Chang,Ziying Li,Feng Wu,Jing He,Tiantian Cao,Kangrong Wang,Nengxian Shi,Hao Zhou,Sam Toan,David Muid,Ying Tan

Nature communications 13:1172 PubMed35246531

2022

Oxygen-sensitive methylation of ULK1 is required for hypoxia-induced autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Jingyi Li,Tao Zhang,Tao Ren,Xiaoyu Liao,Yilong Hao,Je Sun Lim,Jong-Ho Lee,Mi Li,Jichun Shao,Rui Liu

Frontiers in pharmacology 12:763160 PubMed35111047

2022

Activation of Autophagy Through the NLRP3/mTOR Pathway: A Potential Mechanism for Alleviation of Pneumonia by QingFei Yin.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaozhou Sun,Dandan Wang,Lizhong Ding,Yan Xu,Wenxiu Qi,Daqing Zhao,Li Liu,Chengcheng Yin,Changsheng Cui,Zhongtian Wang,Liwei Sun,Liping Sun

Autophagy 18:576-594 PubMed34092182

2021

PknG manipulates host autophagy flux to promote pathogen intracellular survival.

Applications

Unspecified application

Species

Unspecified reactive species

Pupu Ge,Zehui Lei,Yang Yu,Zhe Lu,Lihua Qiang,Qiyao Chai,Yong Zhang,Dongdong Zhao,Bingxi Li,Yu Pang,Cui Hua Liu,Jing Wang

Journal of gastrointestinal oncology 11:1322-1332 PubMed33457004

2021

Propofol activates AMPK to inhibit the growth of HepG2 cells and hepatocarcinogenesis in xenograft mouse tumor models by inducing autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Yixiong Wang,Baozhu Xu,Jianying Zhou,Xianyan Wu

Molecular metabolism 42:101089 PubMed32987187

2020

P2Y2R contributes to the development of diabetic nephropathy by inhibiting autophagy response.

Applications

Unspecified application

Species

Unspecified reactive species

Theodomir Dusabimana,So Ra Kim,Eun Jung Park,Jihyun Je,Kyuho Jeong,Seung Pil Yun,Hye Jung Kim,Hwajin Kim,Sang Won Park
View all publications
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