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AB97695

Anti-ULK2 antibody

2

(1 Review)

|

(14 Publications)

Rabbit Polyclonal ULK2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 14 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ULK2 aa 650-950.

View Alternative Names

KIAA0623, ULK2, Serine/threonine-protein kinase ULK2, Unc-51-like kinase 2

6 Images
Immunocytochemistry/ Immunofluorescence - Anti-ULK2 antibody (AB97695)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ULK2 antibody (AB97695)

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab97695 at 1/200 dilution. Lower image is merged with DNA probe.

Immunocytochemistry/ Immunofluorescence - Anti-ULK2 antibody (AB97695)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ULK2 antibody (AB97695)

Immunofluorescence analysis of HeLa cells fixed in ice-cold MeOH for 5 min labelling ULK2 protein at cytoplasm and nucleus using ab97695 at a 1/500 dilution (Green). Hoechst 33342 was used for nuclear staining (Blue).

Scale bar = 10 µm

Western blot - Anti-ULK2 antibody (AB97695)
  • WB

Supplier Data

Western blot - Anti-ULK2 antibody (AB97695)

Samples were separated by 5 % SDS.

All lanes:

Western blot - Anti-ULK2 antibody (ab97695) at 1/1000 dilution

All lanes:

NT2D1 whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 113 kDa

false

Western blot - Anti-ULK2 antibody (AB97695)
  • WB

Supplier Data

Western blot - Anti-ULK2 antibody (AB97695)

Samples were separated by 7.5 % SDS.

All lanes:

Western blot - Anti-ULK2 antibody (ab97695) at 1/500 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

A431 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 113 kDa

false

Western blot - Anti-ULK2 antibody (AB97695)
  • WB

Supplier Data

Western blot - Anti-ULK2 antibody (AB97695)

Samples were separated by 7.5 % SDS.

All lanes:

Western blot - Anti-ULK2 antibody (ab97695) at 1/1000 dilution

Lane 1:

Non-transfected 293T whole cell extracts at 30 µg

Lanes 2 - 3:

ULK2 shRN transfected 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 113 kDa

false

Western blot - Anti-ULK2 antibody (AB97695)
  • WB

Supplier Data

Western blot - Anti-ULK2 antibody (AB97695)

Samples were separated by 7.5 % SDS.

All lanes:

Western blot - Anti-ULK2 antibody (ab97695) at 1/500 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

A431 whole cell extracts at 30 µg

Lane 3:

HeLa whole cell extracts at 30 µg

Lane 4:

HepG2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 113 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human ULK2 aa 650-950. The exact immunogen used to generate this antibody is proprietary information.

Q8IYT8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ULK2 also known as UNC-51-like kinase 2 is a serine/threonine-protein kinase positioned within cellular processes. With an approximate molecular mass of 124 kDa ULK2 is expressed in various tissues prominently within the brain heart and skeletal muscle. This protein exhibits kinase activity essential for transferring phosphate groups to specific substrates affecting their function and activity.
Biological function summary

ULK2 functions as an important regulator in cellular autophagy the process that removes unnecessary or damaged cellular components. It acts as part of the ULK complex which includes ATG13 FIP200 and ATG101. This complex initiates the autophagy process by responding to nutrient availability and energy status within the cell effectively managing cellular homeostasis and survival.

Pathways

ULK2 is mainly involved in the autophagy pathway and the mTOR signaling pathway. The mTOR pathway influences cell growth and metabolism in response to nutritional signals hence ULK2 interacts with mTORC1 to relay signals important for appropriate autophagy initiation. Additionally ULK2 shares a functional relationship with AMPK which senses cellular energy levels and modulates autophagy in part through ULK2 activation.

ULK2 is associated with neurodegenerative diseases like Alzheimer's disease and can play a role in the pathology of certain cancers. Aberrant ULK2 activity alters autophagic processes contributing to the accumulation of misfolded proteins and impaired cell survival. The protein interacts with p62/SQSTM1 a known player in neurodegenerative disease pathways underlining its relevance to disease mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy : acts both as a downstream effector and a negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK, also acts as a negative regulator of AMPK through phosphorylation of the AMPK subunits PRKAA1, PRKAB2 and PRKAG1. May phosphorylate ATG13/KIAA0652, FRS2, FRS3 and RPTOR; however such data need additional evidences. Not involved in ammonia-induced autophagy or in autophagic response of cerebellar granule neurons (CGN) to low potassium concentration. Plays a role early in neuronal differentiation and is required for granule cell axon formation : may govern axon formation via Ras-like GTPase signaling and through regulation of the Rab5-mediated endocytic pathways within developing axons.
See full target information ULK2
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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

PeerJ 12:e17628 PubMed38952983

2024

ULK2 suppresses ovarian cancer cell migration and invasion by elevating IGFBP3.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoxi Chen,Changxiang Shao,Jing Liu,Huizhen Sun,Bingyi Yao,Chengbin Ma,Han Xu,Weipei Zhu

Cell death discovery 9:366 PubMed37783703

2023

Exogenous Ang-(1-7) inhibits autophagy via HIF-1α/THBS1/BECN1 axis to alleviate chronic intermittent hypoxia-enhanced airway remodelling of asthma.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Ping Zhou,Yi Wang,Shi Qi Li,Jia Qi Zhang,Ying Ni Lin,Xian Wen Sun,Li Na Zhou,Liu Zhang,Fang Ying Lu,Yong Jie Ding,Qing Yun Li

Oxidative medicine and cellular longevity 2022:1740770 PubMed36388165

2022

Mesangial Cell-Derived Exosomal miR-4455 Induces Podocyte Injury in IgA Nephropathy by Targeting ULK2.

Applications

Unspecified application

Species

Unspecified reactive species

Mengjie Yu,Xiaogang Shen,Wenfang He,Danna Zheng,Qiang He,Juan Jin

Autophagy 18:2615-2635 PubMed35253629

2022

Therapeutic targeting of the USP2-E2F4 axis inhibits autophagic machinery essential for zinc homeostasis in cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjing Xiao,Jianqun Wang,Xiaojing Wang,Shuang Cai,Yanhua Guo,Lin Ye,Dan Li,Anpei Hu,Shikai Jin,Boling Yuan,Yi Zhou,Qilan Li,Qiangsong Tong,Liduan Zheng

Nature communications 13:1172 PubMed35246531

2022

Oxygen-sensitive methylation of ULK1 is required for hypoxia-induced autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Jingyi Li,Tao Zhang,Tao Ren,Xiaoyu Liao,Yilong Hao,Je Sun Lim,Jong-Ho Lee,Mi Li,Jichun Shao,Rui Liu

International journal of biological sciences 17:2772-2794 PubMed34345207

2021

Crosstalk between hypoxia-sensing ULK1/2 and YAP-driven glycolysis fuels pancreatic ductal adenocarcinoma development.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Jia,Hui-Yan Li,Ying Wang,Jue Wang,Jing-Wen Zhu,Yan-Yan Wei,Lu Lou,Xing Chen,Shi-Jing Mo

Nucleic acids research 49:6437-6455 PubMed34096600

2021

An essential role of the autophagy activating kinase ULK1 in snRNP biogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Katharina Schmitz,Jan Cox,Lea Marie Esser,Martin Voss,Katja Sander,Antje Löffler,Frank Hillebrand,Steffen Erkelenz,Heiner Schaal,Thilo Kähne,Stefan Klinker,Tao Zhang,Luitgard Nagel-Steger,Dieter Willbold,Sabine Seggewiß,David Schlütermann,Björn Stork,Matthias Grimmler,Sebastian Wesselborg,Christoph Peter

Autophagy 18:142-160 PubMed33966599

2021

Lipotoxicity reduces DDX58/Rig-1 expression and activity leading to impaired autophagy and cell death.

Applications

Unspecified application

Species

Unspecified reactive species

Karla K Frietze,Alyssa M Brown,Dividutta Das,Raymond G Franks,Jessie Lee Cunningham,Michael Hayward,Joseph T Nickels

Cancer management and research 13:871-887 PubMed33551645

2021

Suppressed Proliferation and Promoted Apoptosis in Laryngeal Squamous Cell Carcinoma by Targeting and Modifying ULK2.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Wang,Yiming Zhu,Song Ni,Shaoyan Liu

Molecular medicine reports 22:2460-2468 PubMed32705220

2020

Long non‑coding RNA growth arrest‑specific 5 (GAS5) acts as a tumor suppressor by promoting autophagy in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Guangping Li,Lin Qian,Xiaoqin Tang,Yuan Chen,Ziyi Zhao,Cuiwei Zhang
View all publications
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