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AB34668

Anti-ULP-1 antibody

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(1 Publication)

Rabbit Polyclonal SpUlp1 antibody. Suitable for ICC/IF, WB, ELISA and reacts with Saccharomyces cerevisiae samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Saccharomyces cerevisiae S288C ULP1.

View Alternative Names

YPL020C, LPB11C, ULP1, Ubiquitin-like-specific protease 1

1 Images
Western blot - Anti-ULP-1 antibody (AB34668)
  • WB

Unknown

Western blot - Anti-ULP-1 antibody (AB34668)

Increasing concentrations of ULP 1 were run on SDS-PAGE and transferred onto nitrocellulose. A chemiluminescence system was used for signal detection (Roche) using a 3 seconds exposure time.

All lanes:

Western blot - Anti-ULP-1 antibody (ab34668) at 1/1000 dilution

All lanes:

Saccharomyces cerevisiae truncated ULP 1 fusion protein (arrow-head)

Secondary

All lanes:

HRP-labeledDonkey anti-Rabbit IgG for 1 hour at room temperature. at 1/1000 dilution

Predicted band size: 80 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Saccharomyces cerevisiae

Applications

ICC/IF, ELISA, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Saccharomyces cerevisiae S288C ULP1.

Q02724

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Saccharomyces cerevisiae": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/500 - 1/2000", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1/4000 - 1/20000", "ELISA-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purity
IgG fraction
Purification technique
Ion exchange chromatography
Purification notes
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.
Storage buffer
Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SpUlp1 also known as Ulp1p or Ubl-specific protease 1 is a SUMO-specific protease that has a mass of approximately 80 kDa. It catalyzes the deconjugation of small ubiquitin-like modifier (SUMO) proteins from various substrate proteins. This enzymatic action is important for maintaining protein functionality within the cell. SpUlp1 is expressed in a variety of eukaryotic cells where it localizes to the nuclear envelope and is involved in the dynamic process of SUMO modification.
Biological function summary

The activity of this protease maintains cellular protein homeostasis through reverting sumoylated proteins back to their unmodified states influencing a broad range of cellular functions. SpUlp1 forms part of the SUMO modification pathway and interacts with several other components of the SUMO machinery. Its action serves to modulate processes such as nuclear transport transcription and signal transduction which are essential for cell cycle progression and stress responses.

Pathways

SUMO modification heavily involving SpUlp1 integrates into gene expression regulation and DNA damage repair mechanisms. It operates in the SUMOylation pathway critically impacting processes by interacting with proteins like RanGAP1 and p53. These interactions allow cellular machinery to appropriately respond to environmental and intracellular cues influencing cell survival and repair pathways.

Abnormalities in SpUlp1 activity relate to cancer and neurodegenerative diseases. Altered SUMOylation impacts cellular stress responses and apoptosis factors associated with tumorigenesis and the progression of diseases like Alzheimer's. The target is connected to proteins p53 and tau implicated in these conditions where dysregulation leads to aberrant cell cycle progression and aggregation of neurotoxic proteins.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protease that catalyzes two essential functions in the SUMO pathway : processing of full-length SMT3 to its mature form and deconjugation of SMT3 from targeted proteins. Has an essential role in the G2/M phase of the cell cycle.
See full target information ULP1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

American journal of cancer research 12:5462-5483 PubMed36628281

2022

Sumoylation participates in the regulation of YB-1-mediated mismatch repair deficiency and alkylator tolerance.

Applications

Unspecified application

Species

Unspecified reactive species

Ru-Tsun Mai,Chi-Hong Chao,Yao-Wen Chang,Yu-Ching Kao,Yi Cheng,Hsiang-Yu Hsu,Yi-Yuan Su,Chen-Yun Wang,Bo-Ying Lai
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Product promise

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