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Rabbit Recombinant Monoclonal UMOD antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 9 publications.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UMOD antibody [EPR20071] (AB207170), expandable thumbnail
  • Western blot - Anti-UMOD antibody [EPR20071] (AB207170), expandable thumbnail
  • Western blot - Anti-UMOD antibody [EPR20071] (AB207170), expandable thumbnail
  • Immunoprecipitation - Anti-UMOD antibody [EPR20071] (AB207170), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UMOD antibody [EPR20071] (AB207170), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-P
Human
Tested
Tested
Tested
Mouse
Expected
Tested
Tested
Rat
Expected
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/30
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

5 products for Alternative Product

Target data

Function

Uromodulin. Functions in biogenesis and organization of the apical membrane of epithelial cells of the thick ascending limb of Henle's loop (TALH), where it promotes formation of complex filamentous gel-like structure that may play a role in the water barrier permeability (Probable). May serve as a receptor for binding and endocytosis of cytokines (IL-1, IL-2) and TNF (PubMed:3498215). Facilitates neutrophil migration across renal epithelia (PubMed:20798515). Uromodulin, secreted form. In the urine, may contribute to colloid osmotic pressure, retards passage of positively charged electrolytes, and inhibits formation of liquid containing supersaturated salts and subsequent formation of salt crystals (By similarity). Protects against urinary tract infections by binding to type 1 fimbriated E.coli (PubMed:11134021, PubMed:32616672). Binds to bacterial adhesin fimH which mediates the stable formation of bacterial aggregates, prevents the binding of E.coli to uroplakins UPK1A and UPK1B which act as urothelial receptors for type I fimbriae, and allows for pathogen clearance through micturation (PubMed:11134021, PubMed:32616672). Also promotes aggregation of other bacteria including K.pneumoniae, P.aeruginosa and S.mitis and so may also protect against other uropathogens (PubMed:32616672).

Alternative names

Uromodulin, Tamm-Horsfall urinary glycoprotein, THP, UMOD

Recommended products

Rabbit Recombinant Monoclonal UMOD antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 9 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR20071
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

UMOD also known as uromodulin or Tamm-Horsfall protein is a glycoprotein with an approximate mass of 85 to 90 kDa. This protein is mostly expressed in the thick ascending limb of the loop of Henle in the kidney. Uromodulin is secreted into urine and represents the most abundant protein in normal urine. Its function is to contribute to the water impermeability of the thick ascending limb impact on concentration of urine and provide a barrier to bacteria adhering in the urinary tract.

Biological function summary

Uromodulin plays a role in preventing urinary tract infections by inhibiting the adhesion of bacteria through its glycoprotein structure. It forms a gel-like layer in the urinary tract that traps pathogens. Uromodulin also participates in immune regulation serving as a modulator by interacting with immune cells. Additionally it influences renal handling of ions which impacts blood pressure regulation.

Pathways

Uromodulin is involved in pathways associated with water and salt balance in the kidneys. This protein interacts with NKCC2 the sodium-potassium-chloride cotransporter to mediate ion transport in the thick ascending limb of the loop of Henle. Uromodulin also connects to the renin-angiotensin system helping to regulate blood pressure by influencing sodium reabsorption and vascular functions.

Associated diseases and disorders

Uromodulin is linked to disorders such as familial juvenile hyperuricemic nephropathy and medullary cystic kidney disease type 2. These conditions stem from mutations in the UMOD gene leading to abnormal protein folding. It's noted that UMOD mutations can alter interactions with proteins like NKCC2 impacting ion transport and contributing to the pathophysiology of these kidney disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UMOD antibody [EPR20071] (ab207170), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UMOD antibody [EPR20071] (ab207170)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling UMOD with ab207170 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Strong cytoplasmic staining on distal convoluted is observed [PMID: 23988501].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-UMOD antibody [EPR20071] (ab207170), expandable thumbnail

    Western blot - Anti-UMOD antibody [EPR20071] (ab207170)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The MW observed is consistent with the literature (PMID: 18310901).

    All lanes: Western blot - Anti-UMOD antibody [EPR20071] (ab207170) at 1/10000 dilution

    Lane 1: Human urine at 10 µg

    Lane 2: Human fetal kidney lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution

    Predicted band size: 70 kDa

    Observed band size: 110 kDa

    Exposure time: 5s

  • Western blot - Anti-UMOD antibody [EPR20071] (ab207170), expandable thumbnail

    Western blot - Anti-UMOD antibody [EPR20071] (ab207170)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The MW observed is consistent with the literature (PMID: 18310901).

    All lanes: Western blot - Anti-UMOD antibody [EPR20071] (ab207170) at 1/1000 dilution

    Lane 1: Mouse kidney lysate at 10 µg

    Lane 2: Rat kidney lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 70 kDa

    Observed band size: 110 kDa

    Exposure time: 1s

  • Immunoprecipitation - Anti-UMOD antibody [EPR20071] (ab207170), expandable thumbnail

    Immunoprecipitation - Anti-UMOD antibody [EPR20071] (ab207170)

    UMOD was immunoprecipitated from 0.35 mg of human kidney lysate with ab207170 at 1/30 dilution.

    Western blot was performed from the immunoprecipitate using ab207170 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Human kidney lysate 10μg (Input).

    Lane 2: ab207170 IP in human kidney lysate.

    Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab207170 in human kidney lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 0.5 second.

    All lanes: Immunoprecipitation - Anti-UMOD antibody [EPR20071] (ab207170)

    Predicted band size: 70 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UMOD antibody [EPR20071] (ab207170), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UMOD antibody [EPR20071] (ab207170)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling UMOD with ab207170 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Strong cytoplasmic staining on distal convoluted tubules of mouse kidney is observed [PMID: 23988501].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-UMOD antibody [EPR20071] (ab207170), expandable thumbnail

    Immunoprecipitation - Anti-UMOD antibody [EPR20071] (ab207170)

    UMOD was immunoprecipitated from 0.35 mg of mouse kidney lysate with ab207170 at 1/30 dilution.

    Western blot was performed from the immunoprecipitate using ab207170 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Human kidney lysate 10μg (Input).

    Lane 2: ab207170 IP in human kidney lysate.

    Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab207170 in human kidney lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 0.5 second.

    All lanes: Immunoprecipitation - Anti-UMOD antibody [EPR20071] (ab207170)

    Predicted band size: 70 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UMOD antibody [EPR20071] (ab207170), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UMOD antibody [EPR20071] (ab207170)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling UMOD with ab207170 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Strong cytoplasmic staining on distal convoluted tubules of rat kidney is observed [PMID: 23988501].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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