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Rabbit Recombinant Monoclonal UMOD antibody. Suitable for IP, WB, IHC-Fr, IHC-P and reacts with Mouse, Rat samples.

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Images

Western blot - Anti-UMOD antibody [EPR22887-217] (AB255825), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-UMOD antibody [EPR22887-217] (AB255825), expandable thumbnail
  • Immunoprecipitation - Anti-UMOD antibody [EPR22887-217] (AB255825), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-UMOD antibody [EPR22887-217] (AB255825), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UMOD antibody [EPR22887-217] (AB255825), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-FrIHC-P
Mouse
Tested
Tested
Tested
Tested
Rat
Expected
Tested
Tested
Tested

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/100
Notes

-

Species
Rat
Dilution info
1/100
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

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Target data

Function

Uromodulin. Functions in biogenesis and organization of the apical membrane of epithelial cells of the thick ascending limb of Henle's loop (TALH), where it promotes formation of complex filamentous gel-like structure that may play a role in the water barrier permeability. May serve as a receptor for binding and endocytosis of cytokines (IL-1, IL-2) and TNF. Facilitates neutrophil migration across renal epithelia. Uromodulin, secreted form. In the urine, may contribute to colloid osmotic pressure, retards passage of positively charged electrolytes and inhibits formation of liquid containing supersaturated salts and subsequent formation of salt crystals (PubMed:14871399, PubMed:15327412). Protects against urinary tract infections by binding to type 1 fimbriated E.coli (PubMed:11134021). Binds to the bacterial adhesin fimH which mediates the stable formation of bacterial aggregates, prevents the binding of E.coli to uroplakins UPK1A and UPK1B which act as urothelial receptors for type I fimbriae, and allows for pathogen clearance through micturation (By similarity). Also promotes aggregation of other bacteria including K.pneumoniae, P.aeruginosa and S.mitis and so may also protect against other uropathogens (By similarity).

Alternative names

Uromodulin, Tamm-Horsfall urinary glycoprotein, THP, Umod

Recommended products

Rabbit Recombinant Monoclonal UMOD antibody. Suitable for IP, WB, IHC-Fr, IHC-P and reacts with Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR22887-217
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

UMOD also known as uromodulin or Tamm-Horsfall protein is a glycoprotein with an approximate mass of 85 to 90 kDa. This protein is mostly expressed in the thick ascending limb of the loop of Henle in the kidney. Uromodulin is secreted into urine and represents the most abundant protein in normal urine. Its function is to contribute to the water impermeability of the thick ascending limb impact on concentration of urine and provide a barrier to bacteria adhering in the urinary tract.

Biological function summary

Uromodulin plays a role in preventing urinary tract infections by inhibiting the adhesion of bacteria through its glycoprotein structure. It forms a gel-like layer in the urinary tract that traps pathogens. Uromodulin also participates in immune regulation serving as a modulator by interacting with immune cells. Additionally it influences renal handling of ions which impacts blood pressure regulation.

Pathways

Uromodulin is involved in pathways associated with water and salt balance in the kidneys. This protein interacts with NKCC2 the sodium-potassium-chloride cotransporter to mediate ion transport in the thick ascending limb of the loop of Henle. Uromodulin also connects to the renin-angiotensin system helping to regulate blood pressure by influencing sodium reabsorption and vascular functions.

Associated diseases and disorders

Uromodulin is linked to disorders such as familial juvenile hyperuricemic nephropathy and medullary cystic kidney disease type 2. These conditions stem from mutations in the UMOD gene leading to abnormal protein folding. It's noted that UMOD mutations can alter interactions with proteins like NKCC2 impacting ion transport and contributing to the pathophysiology of these kidney disorders.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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6 product images

  • Western blot - Anti-UMOD antibody [EPR22887-217] (ab255825), expandable thumbnail

    Western blot - Anti-UMOD antibody [EPR22887-217] (ab255825)

    UMOD is a glycoprotein. The molecular weight observed is consistent with what has been described in the literature (PMID: 24097801).

    Exposure time: Lane 1:10 seconds; Lanes 2-3:3 minutes.

    Blocking and Diluting Buffer and concentration: 5% NFDM/TBST.

    All lanes: Western blot - Anti-UMOD antibody [EPR22887-217] (ab255825) at 1/1000 dilution

    Lane 1: Rat urine at 20 µg

    Lane 2: Mouse kidney lysate at 20 µg

    Lane 3: Mouse urine at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 55 kDa, 70 kDa

    Observed band size: 100 kDa, 60 kDa, 90 kDa

  • Immunohistochemistry (Frozen sections) - Anti-UMOD antibody [EPR22887-217] (ab255825), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-UMOD antibody [EPR22887-217] (ab255825)

    Immunohistochemical analysis of 4% PFA fixed, 0.2% Triton X-100 permeabilized frozen Rat kidney tissue labeling UMOD with ab255825 at 1/100 (5.00 μg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluora 488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    Positive staining on the thick ascending limb of the loop of Henle (TALH) in rat kidney (PMID: 28781372) is observed.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution.

  • Immunoprecipitation - Anti-UMOD antibody [EPR22887-217] (ab255825), expandable thumbnail

    Immunoprecipitation - Anti-UMOD antibody [EPR22887-217] (ab255825)

    UMOD was immunoprecipitated from 0.35 mg Mouse urine with ab255825 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab255825 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse urine 10μg

    Lane 2: ab255825 IP in Mouse urine

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab255825 in Mouse urine.

    Blocking and dilution buffer and concentration/ 5% NFDM/TBST.

    Exposure time: 50 seconds.

    All lanes: Immunoprecipitation - Anti-UMOD antibody [EPR22887-217] (ab255825)

    Predicted band size: 70 kDa

    Observed band size: 100 kDa

  • Immunohistochemistry (Frozen sections) - Anti-UMOD antibody [EPR22887-217] (ab255825), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-UMOD antibody [EPR22887-217] (ab255825)

    Immunohistochemical analysis of 4% PFA fixed, 0.2% Triton X-100 permeabilized frozen Mouse kidney tissue labeling UMOD with ab255825 at 1/100 (5.00 μg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    Positive staining on the thick ascending limb of the loop of Henle (TALH) in mouse kidney (PMID: 28781372) is observed.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UMOD antibody [EPR22887-217] (ab255825), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UMOD antibody [EPR22887-217] (ab255825)

    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling UMOD with ab255825 at 1/100 dilution (5.0 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the thick ascending limb of the loop of Henle (TALH) in mouse kidney (PMID: 14569098) is observed. Counterstained with Hematoxylin. Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UMOD antibody [EPR22887-217] (ab255825), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UMOD antibody [EPR22887-217] (ab255825)

    Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling UMOD with ab255825 at 1/100 dilution (5.0 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the thick ascending limb of the loop of Henle (TALH) in rat kidney (PMID: 14569098) is observed. Counterstained with Hematoxylin. Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

    Secondary antibody only control/ Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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