AB97924

Anti-UNC13B antibody

Name: Anti-UNC13B antibody (ab97924) | Abcam
Brand: Abcam Limited
SKU: AB97924
Price: 520 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal UNC13B antibody. Suitable for WB, ICC/IF and reacts with Human, Rat samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human UNC13B.

View Alternative Names

UNC13, UNC13B, Protein unc-13 homolog B, Munc13-2, munc13

2 Images

Unknown

Western blot - Anti-UNC13B antibody (AB97924)

5% SDS Page

All lanes:

Western blot - Anti-UNC13B antibody (ab97924) at 1/500 dilution

All lanes:

293T whole cell lysate at 30 µg

Predicted band size: 180 kDa

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Immunocytochemistry/ Immunofluorescence - Anti-UNC13B antibody (AB97924)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-UNC13B antibody (AB97924)

Immunofluorescence analysis of DIV9 rat E18 primary cortical neuron cells fixed in 4% paraformaldehyde at RT for 15 min labelling UNC13B protein using ab97924 at a 1/500 dilution (Green).
beta Tubulin 3/ Tuj1 antibody was used at 1/500 dilution (Red)
DAPI was used for nuclear staining (Blue).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human UNC13B.

O14795

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UNC13B also known as Munc13-2 is a large protein with a molecular mass of approximately 220 kDa. It plays a critical role in synaptic vesicle fusion and neurotransmitter release at the presynaptic terminal. UNC13B is expressed in the central nervous system particularly in the brain. The protein acts as a scaffold enabling the proper assembly and function of synaptic vesicle-associated molecular machinery.

Biological function summary

The protein interacts closely with SNARE proteins to prime synaptic vesicles for exocytosis. UNC13B is an important component of the synaptic vesicle release machinery functioning as part of larger molecular complexes involved in synaptic transmission. It facilitates the transition of synaptic vesicles from a fusion-competent state to an active state ready for neurotransmitter release. This interaction positions it as significant for efficient neural communication and plasticity.

Pathways

Synaptic vesicle cycle and neurotransmitter release cascade involve UNC13B. It works together with syntaxin-1 and Munc18-1 as part of the SNARE complex machinery. This interaction ensures the transmission of nerve signals by facilitating neurotransmitter release at synaptic junctions. Proper functioning of this pathway is essential for maintaining normal neural circuit activity.

Defects in UNC13B have links to neuropsychiatric conditions like schizophrenia and epilepsy. These diseases often associate with abnormal synaptic transmission and neural communication failures. Research shows that imbalances in the protein's regulatory pathways involving proteins like Synaptotagmin might contribute to these disorders. Understanding UNC13B's precise role might offer insights into potential therapeutic targets for these neural communication-related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. Is involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity-depending refilling of readily releasable vesicle pool (RRP) (By similarity). Essential for synaptic vesicle maturation in a subset of excitatory/glutamatergic but not inhibitory/GABA-mediated synapses (By similarity). In collaboration with UNC13A, facilitates neuronal dense core vesicles fusion as well as controls the location and efficiency of their synaptic release (By similarity).

See full target information UNC13B

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature 603:131-137 PubMed35197628

2022

TDP-43 loss and ALS-risk SNPs drive mis-splicing and depletion of UNC13A.

Applications

Unspecified application

Species

Unspecified reactive species

Anna-Leigh Brown,Oscar G Wilkins,Matthew J Keuss,Sarah E Hill,Matteo Zanovello,Weaverly Colleen Lee,Alexander Bampton,Flora C Y Lee,Laura Masino,Yue A Qi,Sam Bryce-Smith,Ariana Gatt,Martina Hallegger,Delphine Fagegaltier,Hemali Phatnani,Jia Newcombe,Emil K Gustavsson,Sahba Seddighi,Joel F Reyes,Steven L Coon,Daniel Ramos,Giampietro Schiavo,Elizabeth M C Fisher,Towfique Raj,Maria Secrier,Tammaryn Lashley,Jernej Ule,Emanuele Buratti,Jack Humphrey,Michael E Ward,Pietro Fratta

The Journal of biological chemistry 295:12674-12685 PubMed32669361

2020

A comprehensive analysis of Rab GTPases reveals a role for Rab34 in serum starvation-induced primary ciliogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Mai E Oguchi,Koki Okuyama,Yuta Homma,Mitsunori Fukuda

Molecular medicine reports 18:1015-1024 PubMed29767240

2018

Munc13‑4 mediates human neutrophil elastase‑induced airway mucin5AC hypersecretion by interacting with syntaxin2.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Xu,Jia Zhou,Xiang-Dong Zhou,Qi Li,Juliy M Perelman,Victor P Kolosov

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-UNC13B antibody

Western blot - Anti-UNC13B antibody (AB97924)

Immunocytochemistry/ Immunofluorescence - Anti-UNC13B antibody (AB97924)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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