AB189914

Anti-UNC5B antibody [EPR18439]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal UNC5B antibody. Suitable for IP, WB, ICC/IF and reacts with Rat samples. Cited in 3 publications.

View Alternative Names

Unc5h2, Unc5b, Netrin receptor UNC5B, Protein unc-5 homolog 2, Protein unc-5 homolog B

4 Images

Supplier Data

Western blot - Anti-UNC5B antibody [EPR18439] (AB189914)

Blocking/Dilution buffer : 5% NFDM/TBST.

UNC5B was cleaved by caspase-3 at Asp412 to produce a cleavage fragment around ~60kDa Llambi F. et al. EMBO J. 2001 Jun 1; 20(11) : 2715-22.

All lanes:

Western blot - Anti-UNC5B antibody [EPR18439] (ab189914) at 1/10000 dilution

All lanes:

C6 (Rat glial tumor cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 103 kDa

Observed band size: 130 kDa,60 kDa

false

Exposure time: 2min

Immunocytochemistry/ Immunofluorescence - Anti-UNC5B antibody [EPR18439] (AB189914)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-UNC5B antibody [EPR18439] (AB189914)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized C6 (Rat glial tumor cell line) cells labeling UNC5B with ab189914 at 1/500 dilution followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing cytoplasm staining on C6 cell line.

The nuclear counterstain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin antibody - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG(AlexaFluor®594) preadsorbed (ab150120) at 1/1000 dilution (red).

The negative controls are as follows : -

-ve control 1 : ab189914 at 1/500 dilution followed by ab150120 at 1/1000 dilution.

-ve control 2 : ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.

Supplier Data

Immunoprecipitation - Anti-UNC5B antibody [EPR18439] (AB189914)

UNC5B was immunoprecipitated from 1mg of Rat cerebral cortex whole cell extract with ab189914 at 1/50 dilution.

Western blot was performed from the immunoprecipitate using ab189914 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/500 dilution.

Lane 1 : Rat cerebral cortex whole cell extract,10μg (Input).

Lane 2 : ab189914 IP in Rat cerebral cortex whole cell extract.

Lane 3 : Rabbit IgG,monoclonal - Isotype Control (ab172730) instead of ab189914 in Rat cerebral cortex whole cell extract.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-UNC5B antibody [EPR18439] (ab189914)

Predicted band size: 103 kDa

false

Supplier Data

Western blot - Anti-UNC5B antibody [EPR18439] (AB189914)

Blocking/Dilution buffer : 5% NFDM/TBST.

UNC5B was cleaved by caspase-3 at Asp412 to produce a cleavage fragment around ~60kDa Llambi F. et al. EMBO J. 2001 Jun 1; 20(11) : 2715-22.

All lanes:

Western blot - Anti-UNC5B antibody [EPR18439] (ab189914) at 1/2000 dilution

Lane 1:

Rat cerebral cortex tissue lysate at 10 µg

Lane 2:

Rat hippocampus tissue lysate at 10 µg

Lane 3:

Rat hypothalamus tissue lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 103 kDa

Observed band size: 130 kDa,60 kDa

false

Exposure time: 10s

Carrier free

Anti-UNC5B antibody [EPR18439] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18439

Isotype

IgG

Carrier free

Reacts with

Rat

Applications

ICC/IF, IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UNC5B also referred to as Unc-5 homolog B is a member of the UNC5 family of Netrin receptors. This protein acts mechanically as a dependence receptor that modulates axon guidance by mediating repulsion of axons away from the source of its ligand Netrin-1. UNC5B has a molecular mass around 110 kDa. Scientists observe the expression of UNC5B predominantly in endothelial cells and neuronal tissues indicating its role in vascular and nervous system development.

Biological function summary

Scientists understand UNC5B to mediate the anti-angiogenic effects within tissues. It is an essential component of the signaling pathways that govern apoptosis in response to cellular conditions. UNC5B forms part of a larger receptor complex with Netrin-1 which influences cellular directionality and guidance by affecting cytoskeletal dynamics.

Pathways

The UNC5B receptor prominently participates in the Netrin signaling pathway. Within this pathway UNC5B interacts with other proteins such as DCC (deleted in colorectal cancer) that are involved in axonal guidance and neuronal navigation. It also plays a part in the apoptotic signaling pathways which are important for regulating cell survival and programmed cell death.

Researchers associate UNC5B with cancer progression and neurodevelopmental disorders. The regulation of UNC5B is important in the suppression of tumor angiogenesis where its interaction with Netrin-1 can impact cancer cell vascular supply. Additionally misregulation of its pathway might contribute to conditions such as autism spectrum disorders where proteins like DCC interact with it indicating a potential role in neurodevelopmental pathologies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for netrin required for axon guidance. Mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion (PubMed : 10399920, PubMed : 9126742). Functions as a netrin receptor that negatively regulates vascular branching during angiogenesis. Mediates retraction of tip cell filopodia on endothelial growth cones in response to netrin (By similarity). It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand (PubMed : 11387206). Mediates apoptosis by activating DAPK1 (PubMed : 18582460). In the absence of NTN1, activates DAPK1 by reducing its autoinhibitory phosphorylation at Ser-308 thereby increasing its catalytic activity (PubMed : 15729359).

See full target information Unc5b

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Bioengineered 13:12115-12126 PubMed35546072

2022

Far upstream element -binding protein 1 (FUBP1) participates in the malignant process and glycolysis of colon cancer cells by combining with c-Myc.

Applications

Unspecified application

Species

Unspecified reactive species

Shanwei Wang,Yanli Wang,Sheng Li,Shen Nian,Wenjing Xu,Fenli Liang

The Journal of endocrinology 244:445-458 PubMed31846437

2019

Inhibition of vascular adventitial remodeling by netrin-1 in diabetic rats.

Applications

Unspecified application

Species

Unspecified reactive species

Hui-Fang Wang,Qing-Qing Yu,Rui-Fang Zheng,Ming Xu

Frontiers in cellular neuroscience 11:432 PubMed29375318

2018

Neuroprotection Exerted by Netrin-1 and Kinesin Motor KIF1A in Secondary Brain Injury following Experimental Intracerebral Hemorrhage in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Wang,Weiwei Zhai,Zhengquan Yu,Liang Sun,Haiying Li,Haitao Shen,Xiang Li,Chunfeng Liu,Gang Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-UNC5B antibody [EPR18439]

Western blot - Anti-UNC5B antibody [EPR18439] (AB189914)

Immunocytochemistry/ Immunofluorescence - Anti-UNC5B antibody [EPR18439] (AB189914)

Immunoprecipitation - Anti-UNC5B antibody [EPR18439] (AB189914)

Western blot - Anti-UNC5B antibody [EPR18439] (AB189914)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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