Mouse Monoclonal UNC5B antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human UNC5B aa 1-400.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-UNC5B antibody [Guido-1] (AB54430), expandable thumbnail](https://content.abcam.com/products/images/unc5b-antibody-guido-1-ab54430--immunocytochemistry-immunofluorescence-img113996.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-UNC5B antibody [Guido-1] (AB54430)")
Publications
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- Journal of cellular and molecular medicine 25:2121-21352020The intracellular domain of UNC5B facilities proliferation and metastasis of bladder cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYexiang Huang,Zhe Zhang,Miao Miao,Chuize KongPubMed 33345442
- Translational psychiatry 8:2452018Convergence of independent DISC1 mutations on impaired neurite growth via decreased UNC5D expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPriya Srikanth et. al.PubMed 30410030
- The Journal of clinical investigation 126:3006-222016Truncated netrin-1 contributes to pathological vascular permeability in diabetic retinopathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKhalil Miloudi et. al.PubMed 27400127
- Nature medicine 20:377-842014Netrin-1 promotes adipose tissue macrophage retention and insulin resistance in obesity.Applications:IFReactive species:Unspecified reactive speciesBhama Ramkhelawon et. al.PubMed 24584118
- PloS one 7:e410852012The uncoordinated-5 homolog B receptor affects hepatic ischemia reperfusion injury.
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human UNC5B aa 1-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q8IZJ1
Reactivity data
Select an application| ICC/IF | |
|---|---|
| Human | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
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1 product for Alternative Product
Target data
Function
Receptor for netrin required for axon guidance. Mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion (By similarity). Functions as a netrin receptor that negatively regulates vascular branching during angiogenesis. Mediates retraction of tip cell filopodia on endothelial growth cones in response to netrin (By similarity). It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand (PubMed:12598906). Mediates apoptosis by activating DAPK1. In the absence of NTN1, activates DAPK1 by reducing its autoinhibitory phosphorylation at Ser-308 thereby increasing its catalytic activity (By similarity).
Alternative names
P53RDL1, UNC5H2, UNQ1883/PRO4326, UNC5B, Netrin receptor UNC5B, Protein unc-5 homolog 2, Protein unc-5 homolog B, p53-regulated receptor for death and life protein 1, p53RDL1
Recommended products
Mouse Monoclonal UNC5B antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human UNC5B aa 1-400.
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human UNC5B aa 1-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q8IZJ1
- Clone number
- Guido-1
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Purified from TCS.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was changed from ascites to tissue culture supernatant on 22nd May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
UNC5B also referred to as Unc-5 homolog B is a member of the UNC5 family of Netrin receptors. This protein acts mechanically as a dependence receptor that modulates axon guidance by mediating repulsion of axons away from the source of its ligand Netrin-1. UNC5B has a molecular mass around 110 kDa. Scientists observe the expression of UNC5B predominantly in endothelial cells and neuronal tissues indicating its role in vascular and nervous system development.
- Biological function summary
Scientists understand UNC5B to mediate the anti-angiogenic effects within tissues. It is an essential component of the signaling pathways that govern apoptosis in response to cellular conditions. UNC5B forms part of a larger receptor complex with Netrin-1 which influences cellular directionality and guidance by affecting cytoskeletal dynamics.
- Pathways
The UNC5B receptor prominently participates in the Netrin signaling pathway. Within this pathway UNC5B interacts with other proteins such as DCC (deleted in colorectal cancer) that are involved in axonal guidance and neuronal navigation. It also plays a part in the apoptotic signaling pathways which are important for regulating cell survival and programmed cell death.
- Associated diseases and disorders
Researchers associate UNC5B with cancer progression and neurodevelopmental disorders. The regulation of UNC5B is important in the suppression of tumor angiogenesis where its interaction with Netrin-1 can impact cancer cell vascular supply. Additionally misregulation of its pathway might contribute to conditions such as autism spectrum disorders where proteins like DCC interact with it indicating a potential role in neurodevelopmental pathologies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
![Immunocytochemistry/ Immunofluorescence - Anti-UNC5B antibody [Guido-1] (ab54430), expandable thumbnail](https://content.abcam.com/products/images/unc5b-antibody-guido-1-ab54430--immunocytochemistry-immunofluorescence-img113996.jpg "Immunocytochemistry/ Immunofluorescence - Anti-UNC5B antibody [Guido-1] (ab54430)")
Immunocytochemistry/ Immunofluorescence - Anti-UNC5B antibody [Guido-1] (ab54430)
ICC/IF image of ab54430 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54430, 5μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
This image was generated using the ascites version of the product.
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