AB54430

Anti-UNC5B antibody [Guido-1]

Name: Anti-UNC5B antibody [Guido-1] (ab54430) | Abcam
Brand: Abcam Limited
SKU: AB54430
Rating: 4 (2 reviews)

(2 Reviews)

(5 Publications)

Mouse Monoclonal UNC5B antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human UNC5B aa 1-400.

View Alternative Names

P53RDL1, UNC5H2, UNQ1883/PRO4326, UNC5B, Netrin receptor UNC5B, Protein unc-5 homolog 2, Protein unc-5 homolog B, p53-regulated receptor for death and life protein 1, p53RDL1

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-UNC5B antibody [Guido-1] (AB54430)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-UNC5B antibody [Guido-1] (AB54430)

ICC/IF image of ab54430 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54430, 5μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

This image was generated using the ascites version of the product.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

Guido-1

Isotype

IgG2b

Carrier free

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human UNC5B aa 1-400. The exact immunogen used to generate this antibody is proprietary information.

Q8IZJ1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }

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Product details

This product was changed from ascites to tissue culture supernatant on 22^nd May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from TCS.

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UNC5B also referred to as Unc-5 homolog B is a member of the UNC5 family of Netrin receptors. This protein acts mechanically as a dependence receptor that modulates axon guidance by mediating repulsion of axons away from the source of its ligand Netrin-1. UNC5B has a molecular mass around 110 kDa. Scientists observe the expression of UNC5B predominantly in endothelial cells and neuronal tissues indicating its role in vascular and nervous system development.

Biological function summary

Scientists understand UNC5B to mediate the anti-angiogenic effects within tissues. It is an essential component of the signaling pathways that govern apoptosis in response to cellular conditions. UNC5B forms part of a larger receptor complex with Netrin-1 which influences cellular directionality and guidance by affecting cytoskeletal dynamics.

Pathways

The UNC5B receptor prominently participates in the Netrin signaling pathway. Within this pathway UNC5B interacts with other proteins such as DCC (deleted in colorectal cancer) that are involved in axonal guidance and neuronal navigation. It also plays a part in the apoptotic signaling pathways which are important for regulating cell survival and programmed cell death.

Researchers associate UNC5B with cancer progression and neurodevelopmental disorders. The regulation of UNC5B is important in the suppression of tumor angiogenesis where its interaction with Netrin-1 can impact cancer cell vascular supply. Additionally misregulation of its pathway might contribute to conditions such as autism spectrum disorders where proteins like DCC interact with it indicating a potential role in neurodevelopmental pathologies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for netrin required for axon guidance. Mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion (By similarity). Functions as a netrin receptor that negatively regulates vascular branching during angiogenesis. Mediates retraction of tip cell filopodia on endothelial growth cones in response to netrin (By similarity). It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand (PubMed : 12598906). Mediates apoptosis by activating DAPK1. In the absence of NTN1, activates DAPK1 by reducing its autoinhibitory phosphorylation at Ser-308 thereby increasing its catalytic activity (By similarity).

See full target information UNC5B

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 25:2121-2135 PubMed33345442

2020

The intracellular domain of UNC5B facilities proliferation and metastasis of bladder cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yexiang Huang,Zhe Zhang,Miao Miao,Chuize Kong

Translational psychiatry 8:245 PubMed30410030

2018

Convergence of independent DISC1 mutations on impaired neurite growth via decreased UNC5D expression.

Applications

Unspecified application

Species

Unspecified reactive species

Priya Srikanth,Valentina N Lagomarsino,Richard V Pearse,Meichen Liao,Sulagna Ghosh,Ralda Nehme,Nicholas Seyfried,Kevin Eggan,Tracy L Young-Pearse

The Journal of clinical investigation 126:3006-22 PubMed27400127

2016

Truncated netrin-1 contributes to pathological vascular permeability in diabetic retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Khalil Miloudi,François Binet,Ariel Wilson,Agustin Cerani,Malika Oubaha,Catherine Menard,Sullivan Henriques,Gaelle Mawambo,Agnieszka Dejda,Phuong Trang Nguyen,Flavio A Rezende,Steve Bourgault,Timothy E Kennedy,Przemyslaw Sapieha

Nature medicine 20:377-84 PubMed24584118

2014

Netrin-1 promotes adipose tissue macrophage retention and insulin resistance in obesity.

Applications

Species

Unspecified reactive species

Bhama Ramkhelawon,Elizabeth J Hennessy,Mickaël Ménager,Tathagat Dutta Ray,Frederick J Sheedy,Susan Hutchison,Amarylis Wanschel,Scott Oldebeken,Michele Geoffrion,Westley Spiro,George Miller,Ruth McPherson,Katey J Rayner,Kathryn J Moore

PloS one 7:e41085 PubMed22848430

2012

The uncoordinated-5 homolog B receptor affects hepatic ischemia reperfusion injury.

Applications

Flow Cyt

Species

Mouse

Klemens König,David Köhler,Tiago Granja,Carla Jennewein,Nguyen Tran,Valbona Mirakaj,Friedemann Kröhnert,Peter Rosenberger

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-UNC5B antibody [Guido-1]

Immunocytochemistry/ Immunofluorescence - Anti-UNC5B antibody [Guido-1] (AB54430)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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