AB302924

Anti-UNC5C antibody [EPR24190-37]

20ul selling size
RabMAb
Recombinant
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Rabbit Recombinant Monoclonal UNC5C antibody. Suitable for WB and reacts with Human, Mouse, Rat samples.

View Alternative Names

UNC5H3, UNC5C, Netrin receptor UNC5C, Protein unc-5 homolog 3, Protein unc-5 homolog C

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Supplier Data

Western blot - Anti-UNC5C antibody [EPR24190-37] (AB302924)

Blocking and dilution buffer : 5% NFDM/TBST

Negative control : 293T(PMID : 21893118)

The molecular weight observed is consistent with what has been described in the literature (PMID : 27716118; 33863723).

This blot was developed using a high sensitivity ECL substrate in lane 5. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

Samples are non-boiled as boiling may cause protein aggregates.

Exposure time :

Lane 1-4, 6-7 : 180 seconds

Lane 5 : 37 seconds

All lanes:

Western blot - Anti-UNC5C antibody [EPR24190-37] (ab302924) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 50 µg

Lane 2:

Mouse cerebral cortex tissue lysate at 50 µg

Lane 3:

Mouse hippocampus tissue lysate at 50 µg

Lane 4:

Mouse hypothalamus tissue lysate at 50 µg

Lane 5:

Rat brain tissue lysate at 50 µg

Lane 6:

SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate at 50 µg

Lane 7:

293T (human embryonic kidney epithelial cell), whole cell lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 103 kDa

Observed band size: 130 kDa,66 kDa

true

Carrier free

Anti-UNC5C antibody [EPR24190-37] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24190-37

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UNC5C also known as Unc-5 Netrin Receptor C serves as a receptor for netrin a protein that guides axonal growth. It is a member of the UNC5 family and plays an important role in cell migration and positioning. The molecular mass of UNC5C is approximately 103 kDa. It is mainly expressed in the nervous system specifically in the developing brain and spinal cord but can also be found in other tissues to a lesser degree. Its expression pattern suggests a role diverse from just the nervous system.

Biological function summary

UNC5C functions significantly in neuronal guidance and apoptosis regulation. It forms part of a receptor complex together with DCC (Deleted in Colorectal Carcinoma) when binding with netrin-1 another important protein in axon guidance. This interaction influences neuronal cells’ behavior by repelling or attracting axons depending on the context. Beyond its interaction with netrin-1 UNC5C also engages in signaling pathways promoting or inhibiting cell death adding to its versatile functions in cellular processes.

Pathways

UNC5C participates in the axon guidance and apoptotic processes. It plays an important role in the netrin signaling pathway by interacting with DCC and netrin-1 helping to steer neuron growth cones during development. Additionally it has involvement with the apoptosis pathway where UNC5C interactions can lead to programmed cell death when certain conditions are met such as absence of netrin-1. The protein's function is intricately linked with others like DCC and the netrin family which means it influences and is influenced by key neurodevelopmental pathways.

Mutations or dysregulation of UNC5C have been associated with neurodegenerative conditions and cancer. Altered UNC5C signaling can contribute to the progression of Alzheimer's disease potentially through the disruption of normal axonal pathfinding or neuronal death mechanisms. In cancer particularly colorectal cancer UNC5C acts as a dependence receptor where its malfunction can trigger inappropriate cell survival contributing to tumorigenesis. UNC5C’s interaction with DCC highlights its role in colorectal cancer emphasizing how its impairment can promote the escape from normal apoptotic signals.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for netrin required for axon guidance (By similarity). Mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding (By similarity). NTN1/Netrin-1 binding might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion (PubMed : 28483977). Axon repulsion in growth cones may also be caused by its association with DCC that may trigger signaling for repulsion (By similarity). Might also collaborate with DSCAM in NTN1-mediated axon repulsion independently of DCC (By similarity). Also involved in corticospinal tract axon guidance independently of DCC (By similarity). Involved in dorsal root ganglion axon projection towards the spinal cord (PubMed : 28483977). It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand (By similarity).

See full target information UNC5C

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-UNC5C antibody [EPR24190-37]

Western blot - Anti-UNC5C antibody [EPR24190-37] (AB302924)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise