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Rabbit Recombinant Monoclonal UNG antibody. Suitable for Dot, IHC-P, WB and reacts with Synthetic peptide, Human samples.

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Images

Dot Blot - Anti-UNG2 antibody [EPR29004-78] (AB318982), expandable thumbnail
  • Western blot - Anti-UNG2 antibody [EPR29004-78] (AB318982), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UNG2 antibody [EPR29004-78] (AB318982), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UNG2 antibody [EPR29004-78] (AB318982), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UNG2 antibody [EPR29004-78] (AB318982), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
DotIHC-PWB
Human
Expected
Tested
Tested
Mouse
Not recommended
Not recommended
Not recommended
Synthetic peptide
Tested
Not recommended
Not recommended

Tested
Tested

Species
Synthetic peptide
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse, Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Synthetic peptide
Dilution info
-
Notes

-

Target data

Function

Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine.

Alternative names

DGU, UNG1, UNG15, UNG, Uracil-DNA glycosylase, UDG

Recommended products

Rabbit Recombinant Monoclonal UNG antibody. Suitable for Dot, IHC-P, WB and reacts with Synthetic peptide, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR29004-78
Purification technique
Affinity purification Protein A
Specificity

The immunogen is located within the N-terminal region of UNG2 (P13051-1). Based on peptide dot blot data we do not expect this antibody to cross-react with UNG1 (P13051-2).

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

UNG2 also known as uracil-DNA glycosylase 2 is a DNA repair enzyme with a mass of about 36 kDa. It operates mainly in the cell nucleus where it plays an important role in the base excision repair pathway. The enzyme detects and removes uracil from DNA molecules which often forms through deamination of cytosine or incorporation of dUMP instead of dTMP during DNA replication. By cleaving the N-glycosidic bond of uracil UNG2 initiates a repair process that helps maintain genetic integrity.

Biological function summary

The enzyme has a significant role in preserving the accuracy of genetic information. UNG2 works independently but it acts closely with other repair enzymes in the context of DNA damage response. UNG2 excises uracil efficiently facilitating downstream actions by other repair mechanisms which complete the repair process. The enzyme's activity ensures that DNA mutations which may compromise cellular functions or lead to diseases do not accumulate.

Pathways

The enzyme participates actively in the base excision repair pathway. This pathway critical for correcting small base lesions in DNA involves several components including APE1 and DNA polymerase beta. UNG2 collaborates with these proteins to ensure successful repair. Beyond this UNG2's activity is also linked to immunoglobulin gene diversification processes necessary for adaptive immunity thereby involving itself in immunity pathways.

Associated diseases and disorders

Excessive or deficient activity of UNG2 links to certain conditions. Disruption in UNG2 function may contribute to cancer development due to its role in repairing DNA and preventing mutations. The enzyme's actions also intersect with viral infections; certain viruses such as Epstein-Barr virus may manipulate UNG2 function to favor their replication. Exploring these interactions can provide insights into potential therapeutic targets for these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Dot Blot - Anti-UNG2 antibody [EPR29004-78] (ab318982), expandable thumbnail

    Dot Blot - Anti-UNG2 antibody [EPR29004-78] (ab318982)

    UNG2 Dot Blot staining using rabbit Anti-UNG2 antibody

    Dot blot analysis of UNG2 using ab318982 at 1:1000 (0.501 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Peptides are located within the N-terminal region of UNG1 (P13051-2) and UNG2 (P13051-1). This antibody does not cross-react with human UNG1.

    All lanes: Dot Blot - Anti-UNG2 antibody [EPR29004-78] (ab318982) at 1/1000 dilution

    Lane 1: UNG2 peptide

    Lane 2: UNG1 peptide

    Secondary

    All lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Exposure time: 180s

  • Western blot - Anti-UNG2 antibody [EPR29004-78] (ab318982), expandable thumbnail

    Western blot - Anti-UNG2 antibody [EPR29004-78] (ab318982)

    UNG2 Western blot staining using rabbit Anti-UNG2 antibody

    In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.

    All lanes: Western blot - Anti-UNG2 antibody [EPR29004-78] (ab318982) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 50 µg with NFDM/TBST

    Lane 2: HeLa transfected with siRNA specifically targeting UNG2 whole cell lysate at 50 µg with NFDM/TBST

    Lane 3: 293T (human embryonic kidney epithelial cell) whole cell lysate at 50 µg with NFDM/TBST

    Lane 4: Caco-2 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 50 µg with NFDM/TBST

    Lane 5: T-47D (human ductal breast epithelial tumor epithelial cell) whole cell lysate at 50 µg with NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 37 kDa, 124 kDa

    Exposure time: 180s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UNG2 antibody [EPR29004-78] (ab318982), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UNG2 antibody [EPR29004-78] (ab318982)

    Immunohistochemical analysis of paraffin-embedded Human smooth muscle tissue labeling UNG2 with ab318982 at 1/100 (5.33 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: No staining in human smooth muscle.

    The section was incubated with ab318982 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UNG2 antibody [EPR29004-78] (ab318982), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UNG2 antibody [EPR29004-78] (ab318982)

    Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling UNG2 with ab318982 at 1/100 (5.33 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining in human lung cancer.

    The section was incubated with ab318982 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UNG2 antibody [EPR29004-78] (ab318982), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UNG2 antibody [EPR29004-78] (ab318982)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling UNG2 with ab318982 at 1/100 (5.33 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining in human colon.

    The section was incubated with ab318982 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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