Skip to main content

Rabbit Recombinant Monoclonal UNG antibody. Suitable for Dot, IHC-P, WB and reacts with Synthetic peptide, Human samples.

Be the first to review this product! Submit a review

Images

Dot Blot - Anti-UNG2 antibody [EPR29004-78] (AB318982), expandable thumbnail
  • Western blot - Anti-UNG2 antibody [EPR29004-78] (AB318982), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UNG2 antibody [EPR29004-78] (AB318982), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UNG2 antibody [EPR29004-78] (AB318982), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UNG2 antibody [EPR29004-78] (AB318982), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
DotIHC-PWB
Human
Expected
Tested
Tested
Mouse
Not recommended
Not recommended
Not recommended
Synthetic peptide
Tested
Not recommended
Not recommended

Tested
Tested

Species

Synthetic peptide

Dilution info

1/1000

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Mouse, Synthetic peptide

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Mouse, Synthetic peptide

Dilution info

-

Notes

-

Target data

Function

Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine.

Alternative names

Uracil-DNA glycosylase, UDG, UNG15, UNG1, DGU, UNG

Recommended products

Rabbit Recombinant Monoclonal UNG antibody. Suitable for Dot, IHC-P, WB and reacts with Synthetic peptide, Human samples.

Alternative names

Uracil-DNA glycosylase, UDG, UNG15, UNG1, DGU, UNG

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR29004-78

Purification technique

Affinity purification Protein A

Specificity

The immunogen is located within the N-terminal region of UNG2 (P13051-1). Based on peptide dot blot data we do not expect this antibody to cross-react with UNG1 (P13051-2).

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Dot Blot - Anti-UNG2 antibody [EPR29004-78] (ab318982), expandable thumbnail

    Dot Blot - Anti-UNG2 antibody [EPR29004-78] (ab318982)

    Dot blot analysis of UNG2 using ab318982 at 1:1000 (0.501 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.

    Peptides are located within the N-terminal region of UNG1 (P13051-2) and UNG2 (P13051-1). This antibody does not cross-react with human UNG1.

    All lanes: Dot Blot - Anti-UNG2 antibody [EPR29004-78] (ab318982) at 1/1000 dilution

    Lane 1: UNG2 peptide

    Lane 2: UNG1 peptide

    Secondary

    All lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Exposure time: 180s

  • Western blot - Anti-UNG2 antibody [EPR29004-78] (ab318982), expandable thumbnail

    Western blot - Anti-UNG2 antibody [EPR29004-78] (ab318982)

    In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.

    All lanes: Western blot - Anti-UNG2 antibody [EPR29004-78] (ab318982) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 50 µg

    Lane 2: HeLa transfected with siRNA specifically targeting UNG2 whole cell lysate at 50 µg

    Lane 3: 293T (human embryonic kidney epithelial cell) whole cell lysate at 50 µg

    Lane 4: Caco-2 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 50 µg

    Lane 5: T-47D (human ductal breast epithelial tumor epithelial cell) whole cell lysate at 50 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 37 kDa, 124 kDa

    Exposure time: 180s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UNG2 antibody [EPR29004-78] (ab318982), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UNG2 antibody [EPR29004-78] (ab318982)

    Immunohistochemical analysis of paraffin-embedded Human smooth muscle tissue labeling UNG2 with ab318982 at 1/100 (5.33 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: No staining in human smooth muscle.

    The section was incubated with ab318982 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UNG2 antibody [EPR29004-78] (ab318982), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UNG2 antibody [EPR29004-78] (ab318982)

    Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling UNG2 with ab318982 at 1/100 (5.33 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining in human lung cancer.

    The section was incubated with ab318982 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UNG2 antibody [EPR29004-78] (ab318982), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UNG2 antibody [EPR29004-78] (ab318982)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling UNG2 with ab318982 at 1/100 (5.33 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining in human colon.

    The section was incubated with ab318982 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com