Rabbit Recombinant Monoclonal UNG antibody. Carrier free. Suitable for Dot, IHC-P, WB and reacts with Synthetic peptide, Human samples.
Images
![Dot Blot - Anti-UNG2 antibody [EPR29004-78] - BSA and Azide free (AB318983), expandable thumbnail](https://content.abcam.com/products/images/ung2-antibody-epr29004-78-bsa-and-azide-free-ab318983--dot-blot-img433733.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-UNG2 antibody [EPR29004-78] - BSA and Azide free (AB318983)")
![Western blot - Anti-UNG2 antibody [EPR29004-78] - BSA and Azide free (AB318983), expandable thumbnail](https://content.abcam.com/products/images/ung2-antibody-epr29004-78-bsa-and-azide-free-ab318983--western-blot-img433691.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-UNG2 antibody [EPR29004-78] - BSA and Azide free (AB318983)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UNG2 antibody [EPR29004-78] - BSA and Azide free (AB318983), expandable thumbnail](https://content.abcam.com/products/images/ung2-antibody-epr29004-78-bsa-and-azide-free-ab318983--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img433713.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UNG2 antibody [EPR29004-78] - BSA and Azide free (AB318983)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UNG2 antibody [EPR29004-78] - BSA and Azide free (AB318983), expandable thumbnail](https://content.abcam.com/products/images/ung2-antibody-epr29004-78-bsa-and-azide-free-ab318983--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img433712.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UNG2 antibody [EPR29004-78] - BSA and Azide free (AB318983)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UNG2 antibody [EPR29004-78] - BSA and Azide free (AB318983), expandable thumbnail](https://content.abcam.com/products/images/ung2-antibody-epr29004-78-bsa-and-azide-free-ab318983--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img433711.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UNG2 antibody [EPR29004-78] - BSA and Azide free (AB318983)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Dot | IHC-P | WB | |
|---|---|---|---|
| Human | Expected | Tested | Tested |
| Mouse | Not recommended | Not recommended | Not recommended |
| Synthetic peptide | Tested | Not recommended | Not recommended |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Synthetic peptide | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Synthetic peptide | Dilution info - | Notes - |
Target data
Function
Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine.
Alternative names
Uracil-DNA glycosylase, UDG, UNG15, UNG1, DGU, UNG
Recommended products
Rabbit Recombinant Monoclonal UNG antibody. Carrier free. Suitable for Dot, IHC-P, WB and reacts with Synthetic peptide, Human samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR29004-78
- Purification technique
Affinity purification Protein A
- Specificity
The immunogen is located within the N-terminal region of UNG2 (P13051-1). Based on peptide dot blot data we do not expect this antibody to cross-react with UNG1 (P13051-2).
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
ab318983 is the carrier-free version of Anti-UNG2 antibody [EPR29004-78] ab318982.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
UNG2 also known as uracil-DNA glycosylase 2 is a DNA repair enzyme with a mass of about 36 kDa. It operates mainly in the cell nucleus where it plays an important role in the base excision repair pathway. The enzyme detects and removes uracil from DNA molecules which often forms through deamination of cytosine or incorporation of dUMP instead of dTMP during DNA replication. By cleaving the N-glycosidic bond of uracil UNG2 initiates a repair process that helps maintain genetic integrity.
- Biological function summary
The enzyme has a significant role in preserving the accuracy of genetic information. UNG2 works independently but it acts closely with other repair enzymes in the context of DNA damage response. UNG2 excises uracil efficiently facilitating downstream actions by other repair mechanisms which complete the repair process. The enzyme's activity ensures that DNA mutations which may compromise cellular functions or lead to diseases do not accumulate.
- Pathways
The enzyme participates actively in the base excision repair pathway. This pathway critical for correcting small base lesions in DNA involves several components including APE1 and DNA polymerase beta. UNG2 collaborates with these proteins to ensure successful repair. Beyond this UNG2's activity is also linked to immunoglobulin gene diversification processes necessary for adaptive immunity thereby involving itself in immunity pathways.
- Associated diseases and disorders
Excessive or deficient activity of UNG2 links to certain conditions. Disruption in UNG2 function may contribute to cancer development due to its role in repairing DNA and preventing mutations. The enzyme's actions also intersect with viral infections; certain viruses such as Epstein-Barr virus may manipulate UNG2 function to favor their replication. Exploring these interactions can provide insights into potential therapeutic targets for these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Dot Blot - Anti-UNG2 antibody [EPR29004-78] - BSA and Azide free (ab318983), expandable thumbnail](https://content.abcam.com/products/images/ung2-antibody-epr29004-78-bsa-and-azide-free-ab318983--dot-blot-img433733.jpg "Dot Blot - Anti-UNG2 antibody [EPR29004-78] - BSA and Azide free (ab318983)")
Dot Blot - Anti-UNG2 antibody [EPR29004-78] - BSA and Azide free (ab318983)
This data was developed using Anti-UNG2 antibody [EPR29004-78] ab318982, the same antibody clone in a different buffer formulation.
Dot blot analysis of UNG2 using Anti-UNG2 antibody [EPR29004-78] ab318982 at 1:1000 (0.501 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.
Peptides are located within the N-terminal region of UNG1 (P13051-2) and UNG2 (P13051-1). This antibody does not cross-react with human UNG1.
All lanes: Dot Blot - Anti-UNG2 antibody [EPR29004-78] (Anti-UNG2 antibody [EPR29004-78] ab318982) at 1/1000 dilution
Lane 1: UNG2 peptide
Lane 2: UNG1 peptide
SecondaryAll lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Exposure time: 180s
Dot blot analysis of UNG2 using Anti-UNG2 antibody [EPR29004-78] ab318982 at 1:1000 (0.501 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.
Peptides are located within the N-terminal region of UNG1 (P13051-2) and UNG2 (P13051-1). This antibody does not cross-react with human UNG1.
![Western blot - Anti-UNG2 antibody [EPR29004-78] - BSA and Azide free (ab318983), expandable thumbnail](https://content.abcam.com/products/images/ung2-antibody-epr29004-78-bsa-and-azide-free-ab318983--western-blot-img433691.jpg "Western blot - Anti-UNG2 antibody [EPR29004-78] - BSA and Azide free (ab318983)")
Western blot - Anti-UNG2 antibody [EPR29004-78] - BSA and Azide free (ab318983)
This data was developed using Anti-UNG2 antibody [EPR29004-78] ab318982, the same antibody clone in a different buffer formulation.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.
All lanes: Western blot - Anti-UNG2 antibody [EPR29004-78] (Anti-UNG2 antibody [EPR29004-78] ab318982) at 1/1000 dilution
Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 50 µg
Lane 2: HeLa transfected with siRNA specifically targeting UNG2 whole cell lysate at 50 µg
Lane 3: 293T (human embryonic kidney epithelial cell) whole cell lysate at 50 µg
Lane 4: Caco-2 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 50 µg
Lane 5: T-47D (human ductal breast epithelial tumor epithelial cell) whole cell lysate at 50 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 37 kDa, 124 kDa
Exposure time: 180s
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UNG2 antibody [EPR29004-78] - BSA and Azide free (ab318983), expandable thumbnail](https://content.abcam.com/products/images/ung2-antibody-epr29004-78-bsa-and-azide-free-ab318983--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img433713.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UNG2 antibody [EPR29004-78] - BSA and Azide free (ab318983)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UNG2 antibody [EPR29004-78] - BSA and Azide free (ab318983)
This data was developed using Anti-UNG2 antibody [EPR29004-78] ab318982, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human smooth muscle tissue labeling UNG2 with Anti-UNG2 antibody [EPR29004-78] ab318982 at 1/100 (5.33 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: No staining in human smooth muscle.
The section was incubated with Anti-UNG2 antibody [EPR29004-78] ab318982 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UNG2 antibody [EPR29004-78] - BSA and Azide free (ab318983), expandable thumbnail](https://content.abcam.com/products/images/ung2-antibody-epr29004-78-bsa-and-azide-free-ab318983--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img433712.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UNG2 antibody [EPR29004-78] - BSA and Azide free (ab318983)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UNG2 antibody [EPR29004-78] - BSA and Azide free (ab318983)
This data was developed using Anti-UNG2 antibody [EPR29004-78] ab318982, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling UNG2 with Anti-UNG2 antibody [EPR29004-78] ab318982 at 1/100 (5.33 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in human lung cancer.
The section was incubated with Anti-UNG2 antibody [EPR29004-78] ab318982 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UNG2 antibody [EPR29004-78] - BSA and Azide free (ab318983), expandable thumbnail](https://content.abcam.com/products/images/ung2-antibody-epr29004-78-bsa-and-azide-free-ab318983--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img433711.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UNG2 antibody [EPR29004-78] - BSA and Azide free (ab318983)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UNG2 antibody [EPR29004-78] - BSA and Azide free (ab318983)
This data was developed using Anti-UNG2 antibody [EPR29004-78] ab318982, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling UNG2 with Anti-UNG2 antibody [EPR29004-78] ab318982 at 1/100 (5.33 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in human colon.
The section was incubated with Anti-UNG2 antibody [EPR29004-78] ab318982 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
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