Anti-UNG2 antibody [EPR29004-78] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UNG2 antibody [EPR29004-78] - BSA and Azide free (AB318983)

This data was developed using ab318982, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human smooth muscle tissue labeling UNG2 with ab318982 at 1/100 (5.33 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining in human smooth muscle.
The section was incubated with ab318982 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UNG2 antibody [EPR29004-78] - BSA and Azide free (AB318983)

This data was developed using ab318982, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling UNG2 with ab318982 at 1/100 (5.33 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in human lung cancer.
The section was incubated with ab318982 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UNG2 antibody [EPR29004-78] - BSA and Azide free (AB318983)

This data was developed using ab318982, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling UNG2 with ab318982 at 1/100 (5.33 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in human colon.
The section was incubated with ab318982 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-UNG2 antibody [EPR29004-78] - BSA and Azide free (AB318983)

This data was developed using ab318982, the same antibody clone in a different buffer formulation.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-UNG2 antibody [EPR29004-78] (<a href='/en-us/products/primary-antibodies/ung2-antibody-epr29004-78-ab318982'>ab318982</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 50 µg

Lane 2:

HeLa transfected with siRNA specifically targeting UNG2 whole cell lysate at 50 µg

Lane 3:

293T (human embryonic kidney epithelial cell) whole cell lysate at 50 µg

Lane 4:

Caco-2 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 50 µg

Lane 5:

T-47D (human ductal breast epithelial tumor epithelial cell) whole cell lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 37 kDa,124 kDa

false

Exposure time: 180s

• Dot

Supplier Data

Dot Blot - Anti-UNG2 antibody [EPR29004-78] - BSA and Azide free (AB318983)

This data was developed using ab318982, the same antibody clone in a different buffer formulation.

Dot blot analysis of UNG2 using ab318982 at 1 : 1000 (0.501 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Peptides are located within the N-terminal region of UNG1 (P13051-2) and UNG2 (P13051-1). This antibody does not cross-react with human UNG1.

All lanes:

Dot Blot - Anti-UNG2 antibody [EPR29004-78] (<a href='/en-us/products/primary-antibodies/ung2-antibody-epr29004-78-ab318982'>ab318982</a>) at 1/1000 dilution

Lane 1:

UNG2 peptide

Lane 2:

UNG1 peptide

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s