AB205021

Anti-UNK unkempt antibody

Name: Anti-UNK unkempt antibody (ab205021) | Abcam
Brand: Abcam Limited
SKU: AB205021
Price: 510 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal UNK unkempt antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human UNK aa 750 to C-terminus.

View Alternative Names

KIAA1753, ZC3H5, ZC3HDC5, UNK, RING finger protein unkempt homolog, Zinc finger CCCH domain-containing protein 5

2 Images

Supplier Data

Immunoprecipitation - Anti-UNK unkempt antibody (AB205021)

Immunoprecipitation of UNK unkempt from 1 mg 293T cell lysates using 6 μg per reaction of positive control antibody (lane 1), ab205021 (lane 2) or control IgG (lane 3). For western blotting ab205021 was used at 0.4 μg/mL.

All lanes:

Immunoprecipitation - Anti-UNK unkempt antibody (ab205021)

Predicted band size: 88 kDa

true

Exposure time: 30s

Supplier Data

Western blot - Anti-UNK unkempt antibody (AB205021)

All lanes:

Western blot - Anti-UNK unkempt antibody (ab205021) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

TCMK whole cell lysate at 50 µg

Lane 5:

NIH-3T3 whole cell lysate at 50 µg

Predicted band size: 88 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human UNK aa 750 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9C0B0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UNK also known as unkempt is a protein characterized by its mass of about 105 kDa. It performs as a transcription factor influencing gene expression by binding to DNA and regulating transcription processes. This protein shows a significant expression in neuronal tissues indicating its role in neural development and function. Its ability to interact with specific nucleic acid sequences allows it to modulate the activity of target genes critical for cellular processes.

Biological function summary

Transcription factors like UNK are important in various cellular functions impacting cell differentiation and development. UNK contributes to neural development by interacting with other proteins to form complexes that regulate the expression of genes necessary for neural maintenance and differentiation. It plays a role in controlling the expression of genes that are essential for the development and function of the nervous system.

Pathways

Transcription factors such as UNK influence several signaling cascades and in particular UNK is involved in the MAPK signaling pathway. Through this pathway UNK interacts with proteins like ERK which are important in transmitting signals from surface receptors to the DNA in the cell nucleus affecting gene expression. These interactions integrate various external signals adjusting cellular functions like growth and division.

UNK's role has implications in neurodevelopmental disorders including autism spectrum disorder (ASD). Dysfunctional regulation of gene expression linked to UNK can contribute to the pathology of such conditions. Additionally its association with ZNF family proteins highlights its potential involvement in intellectual disabilities. The misregulation of these proteins can disturb normal neuronal function resulting in cognitive deficits.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Sequence-specific RNA-binding protein which plays an important role in the establishment and maintenance of the early morphology of cortical neurons during embryonic development. Acts as a translation repressor and controls a translationally regulated cell morphology program to ensure proper structuring of the nervous system. Translational control depends on recognition of its binding element within target mRNAs which consists of a mandatory UAG trimer upstream of a U/A-rich motif. Associated with polysomes (PubMed : 25737280).

See full target information UNK

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature 622:173-179 PubMed37731000

2023

Acetyl-methyllysine marks chromatin at active transcription start sites.

Applications

Unspecified application

Species

Unspecified reactive species

William J Lu-Culligan,Leah J Connor,Yixuan Xie,Babatunde E Ekundayo,Brendan T Rose,Martin Machyna,Andreas P Pintado-Urbanc,Joshua T Zimmer,Isaac W Vock,Natarajan V Bhanu,Megan C King,Benjamin A Garcia,Franziska Bleichert,Matthew D Simon

Cell reports 26:733-747.e3 PubMed30650363

2019

Headcase and Unkempt Regulate Tissue Growth and Cell Cycle Progression in Response to Nutrient Restriction.

Applications

Unspecified application

Species

Unspecified reactive species

Naren Li,Qinfang Liu,Yulan Xiong,Jianzhong Yu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-UNK unkempt antibody

Immunoprecipitation - Anti-UNK unkempt antibody (AB205021)

Western blot - Anti-UNK unkempt antibody (AB205021)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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