AB102001

Anti-Unrip antibody

Name: Anti-Unrip antibody (ab102001) | Abcam
Brand: Abcam Limited
SKU: AB102001
Rating: 5 (3 reviews)

(3 Reviews)

(2 Publications)

Rabbit Polyclonal Unrip antibody. Suitable for ICC/IF, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human STRAP aa 1-300.

View Alternative Names

MAWD, UNRIP, STRAP, Serine-threonine kinase receptor-associated protein, MAP activator with WD repeats, UNR-interacting protein, WD-40 repeat protein PT-WD

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-Unrip antibody (AB102001)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Unrip antibody (AB102001)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling STRAP protein at cytoplasm using ab102001 at a 1/500 dilution (Green). Hoechst 33342 was used for nuclear staining (Blue).

Supplier Data

Western blot - Anti-Unrip antibody (AB102001)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Unrip antibody (ab102001) at 1/1000 dilution

Lane 1:

MCF-7 whole cell lysate at 30 µg

Lane 2:

MDA MB 231 whole cell lysate at 30 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 38 kDa

false

Supplier Data

Western blot - Anti-Unrip antibody (AB102001)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Unrip antibody (ab102001) at 1/1000 dilution

Lane 1:

293T whole cell lysates at 30 µg

Lane 2:

A431 whole cell lysates at 30 µg

Lane 3:

HeLa whole cell lysates at 30 µg

Lane 4:

HepG2 whole cell lysates at 30 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 38 kDa

false

Supplier Data

Western blot - Anti-Unrip antibody (AB102001)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Unrip antibody (ab102001) at 1/500 dilution

Lane 1:

Wild-type (WT) 293T cell extracts at 30 µg

Lane 2:

STRAP knockout (KO) 293T cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human STRAP aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

Q9Y3F4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Unrip also known as NRIP (nuclear receptor interaction protein) is a nuclear protein with a molecular mass of approximately 64 kDa. Researchers have identified its expression mainly in the nucleus across various tissues. Mechanically Unrip functions as a factor interacting with nuclear hormone receptors. It regulates transcriptional activity through direct contact with these receptors modulating gene expression. This interaction plays a role in how cells respond to specific hormones and signals.

Biological function summary

Unrip contributes significantly to the regulation of gene expression by associating with other nuclear coregulators. It is part of a larger protein complex that includes importin beta and RIC1/RAB1. This complex facilitates the proper localization and functioning of nuclear hormone receptors affecting the expression of target genes. Unrip’s involvement with such complexes illustrates its integral function in transcriptional regulation and cellular signaling pathways.

Pathways

Unrip integrates into essential signaling networks particularly the nuclear receptor signaling and coactivator pathways. In these pathways Unrip modulates the activity of nuclear receptors like estrogen receptor alpha and glucocorticoid receptor affecting cellular responses to steroid hormones. Unrip interacts with proteins such as SRC-1 and GRIP1 within these pathways influencing gene expression profiles related to growth differentiation and metabolic processes.

Unrip’s interactions impact conditions including cancer and hormone-related disorders. Its role in modulating estrogen and glucocorticoid receptors links it to breast cancer progression where these pathways are dysregulated. Additionally Unrip’s involvement in nuclear receptor signaling ties it to metabolic disorders like type 2 diabetes. Through the alteration of receptor pathways Unrip affects proteins like PPARγ demonstrating its relevance in disease mechanisms and therapeutic research.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP (Sm core). In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. To assemble core snRNPs, the SMN complex accepts the trapped 5Sm proteins from CLNS1A forming an intermediate. Binding of snRNA inside 5Sm triggers eviction of the SMN complex, thereby allowing binding of SNRPD3 and SNRPB to complete assembly of the core snRNP. STRAP plays a role in the cellular distribution of the SMN complex. Negatively regulates TGF-beta signaling but positively regulates the PDPK1 kinase activity by enhancing its autophosphorylation and by significantly reducing the association of PDPK1 with 14-3-3 protein.

See full target information STRAP

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in molecular biosciences 7:55 PubMed32363199

2020

The Small-Molecule Flunarizine in Spinal Muscular Atrophy Patient Fibroblasts Impacts on the Gemin Components of the SMN Complex and TDP43, an RNA-Binding Protein Relevant to Motor Neuron Diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Delphine Sapaly,Perrine Delers,Jennifer Coridon,Badih Salman,Franck Letourneur,Florent Dumont,Suzie Lefebvre

Cancer chemotherapy and pharmacology 82:1039-1047 PubMed30276452

2018

Oncogenic roles of serine-threonine kinase receptor-associated protein (STRAP) in osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Dumnoensun Pruksakorn,Jeerawan Klangjorhor,Kriengsak Lirdprapamongkol,Pimpisa Teeyakasem,Patsadakorn Sungngam,Parunya Chaiyawat,Areerak Phanphaisarn,Jongkolnee Settakorn,Chantragan Srisomsap

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Unrip antibody

Immunocytochemistry/ Immunofluorescence - Anti-Unrip antibody (AB102001)

Western blot - Anti-Unrip antibody (AB102001)

Western blot - Anti-Unrip antibody (AB102001)

Western blot - Anti-Unrip antibody (AB102001)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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