Rabbit Recombinant Monoclonal Urokinase antibody. Suitable for ICC/IF, WB and reacts with Human samples. Cited in 14 publications.
Images
![Western blot - Anti-uPA antibody [EP6274(2)] (AB169754), expandable thumbnail](https://content.abcam.com/products/images/upa-antibody-ep62742-ab169754--western-blot-img99061.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-uPA antibody [EP6274(2)] (AB169754)")
![Immunocytochemistry/ Immunofluorescence - Anti-uPA antibody [EP6274(2)] (AB169754), expandable thumbnail](https://content.abcam.com/products/images/upa-antibody-ep62742-ab169754--immunocytochemistry-immunofluorescence-img68179.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-uPA antibody [EP6274(2)] (AB169754)")
Publications
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- Frontiers in oncology 12:8352992022Hypermethylation of the Promoter Region of miR-23 Enhances the Metastasis and Proliferation of Multiple Myeloma Cells the Aberrant Expression of uPA.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQijie Ran,Dehong Xu,Qi Wang,Dongsheng WangPubMed 35707350
- Cancers 14:2022Molecular Landscape of the Coagulome of Oral Squamous Cell Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarine Lottin,Simon Soudet,Julie Fercot,Floriane Racine,Julien Demagny,Jérémie Bettoni,Denis Chatelain,Marie-Antoinette Sevestre,Youcef Mammeri,Michele Lamuraglia,Antoine Galmiche,Zuzana SaidakPubMed 35053621
- Genes 12:2021Two Rare Variants in and Genes-Do They Contribute to Semantic Dementia Clinical Phenotype?Applications:Unspecified applicationReactive species:Unspecified reactive speciesKatarzyna Gaweda-Walerych,Emilia J Sitek,Małgorzata Borczyk,Mariusz Berdyński,Ewa Narożańska,Bogna Brockhuis,Michał Korostyński,Jarosław Sławek,Cezary ZekanowskiPubMed 34828412
- Bioengineered 12:9753-97652021Long non-coding RNA TRPM2-AS regulates microRNA miR-138-5p and PLAU (Plasminogen Activator, Urokinase) to promote the progression of gastric adenocarcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Sun,Fang Zhou,Juan Xue,Chunyan Ji,Yinzong Qu,Yuwei PanPubMed 34696681
- Cell death discovery 7:322021PLAU directs conversion of fibroblasts to inflammatory cancer-associated fibroblasts, promoting esophageal squamous cell carcinoma progression via uPAR/Akt/NF-κB/IL8 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLingling Fang,Yun Che,Chaoqi Zhang,Jianbing Huang,Yuanyuan Lei,Zhiliang Lu,Nan Sun,Jie HePubMed 33574243
- Pharmacological research 143:73-852019Novel urokinase-plasminogen activator inhibitor SPINK13 inhibits growth and metastasis of hepatocellular carcinoma in vivo.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLing Wei et. al.PubMed 30862605
- OncoTargets and therapy 11:7733-77432018MicroRNA-645 targets urokinase plasminogen activator and decreases the invasive growth of MDA-MB-231 triple-negative breast cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDu Meng et. al.PubMed 30464522
- Oncology research 27:515-5242018Upregulation of miR-324-5p Inhibits Proliferation and Invasion of Colorectal Cancer Cells by Targeting ELAVL1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChijiang Gu,Mingyuan Zhang,Weiliang Sun,Changzheng DongPubMed 29386086
- Scientific reports 7:135702017De novo mutations of TUBA3D are associated with keratoconus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiao-Dan Hao et. al.PubMed 29051577
- Oncology letters 14:7288-72962017Overexpression of forkhead box M1 and urokinase-type plasminogen activator in gastric cancer is associated with cancer progression and poor prognosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJie Ma et. al.PubMed 29344165
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | Flow Cyt | WB | |
|---|---|---|---|
| Human | Tested | Not recommended | Tested |
| Chimpanzee | Predicted | Not recommended | Predicted |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Chimpanzee | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/5000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee | Dilution info - | Notes - |
Target data
Function
Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.
Alternative names
Urokinase-type plasminogen activator, U-plasminogen activator, uPA, PLAU
Recommended products
Rabbit Recombinant Monoclonal Urokinase antibody. Suitable for ICC/IF, WB and reacts with Human samples. Cited in 14 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EP6274(2)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-uPA antibody [EP6274(2)] (ab169754), expandable thumbnail](https://content.abcam.com/products/images/upa-antibody-ep62742-ab169754--western-blot-img99061.jpg "Western blot - Anti-uPA antibody [EP6274(2)] (ab169754)")
Western blot - Anti-uPA antibody [EP6274(2)] (ab169754)
Blocking buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-uPA antibody [EP6274(2)] (ab169754) at 1/1000 dilution
All lanes: PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysate at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 49 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-uPA antibody [EP6274(2)] (ab169754), expandable thumbnail](https://content.abcam.com/products/images/upa-antibody-ep62742-ab169754--immunocytochemistry-immunofluorescence-img68179.jpg "Immunocytochemistry/ Immunofluorescence - Anti-uPA antibody [EP6274(2)] (ab169754)")
Immunocytochemistry/ Immunofluorescence - Anti-uPA antibody [EP6274(2)] (ab169754)
Immunocytochemistry analysis of PC-3 (Human prostate adenocarcinoma epithelial cell) cells labeling uPA with Purified ab169754 at 1:50 dilution (3.78 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488,Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Product protocols
- Visit the general protocols
- Visit troubleshooting
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