AB169754

Anti-uPA antibody [EP6274(2)]

Name: Anti-uPA antibody [EP6274(2)] (ab169754) | Abcam
Brand: Abcam Limited
SKU: AB169754
Price: 205 USD
Availability: InStock
Rating: 1 (1 reviews)

20ul selling size
RabMAb
Recombinant
What is this?

(1 Review)

(14 Publications)

Rabbit Recombinant Monoclonal Urokinase antibody. Suitable for ICC/IF, WB and reacts with Human samples. Cited in 14 publications.

View Alternative Names

Urokinase-type plasminogen activator, U-plasminogen activator, uPA, PLAU

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-uPA antibody [EP6274(2)] (AB169754)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-uPA antibody [EP6274(2)] (AB169754)

Immunocytochemistry analysis of PC-3 (Human prostate adenocarcinoma epithelial cell) cells labeling uPA with Purified ab169754 at 1 : 50 dilution (3.78 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488,ab150077) was used as the secondary antibody at 1 : 1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Unknown

Western blot - Anti-uPA antibody [EP6274(2)] (AB169754)

Blocking buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-uPA antibody [EP6274(2)] (ab169754) at 1/1000 dilution

All lanes:

PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 48 kDa

Observed band size: 49 kDa

false

Carrier free

Anti-uPA antibody [EP6274(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP6274(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" }, "Chimpanzee": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.

See full target information Urokinase-type plasminogen activator

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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 12:835299 PubMed35707350

2022

Hypermethylation of the Promoter Region of miR-23 Enhances the Metastasis and Proliferation of Multiple Myeloma Cells the Aberrant Expression of uPA.

Applications

Unspecified application

Species

Unspecified reactive species

Qijie Ran,Dehong Xu,Qi Wang,Dongsheng Wang

Cancers 14: PubMed35053621

2022

Molecular Landscape of the Coagulome of Oral Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Marine Lottin,Simon Soudet,Julie Fercot,Floriane Racine,Julien Demagny,Jérémie Bettoni,Denis Chatelain,Marie-Antoinette Sevestre,Youcef Mammeri,Michele Lamuraglia,Antoine Galmiche,Zuzana Saidak

Genes 12: PubMed34828412

2021

Two Rare Variants in and Genes-Do They Contribute to Semantic Dementia Clinical Phenotype?

Applications

Unspecified application

Species

Unspecified reactive species

Katarzyna Gaweda-Walerych,Emilia J Sitek,Małgorzata Borczyk,Mariusz Berdyński,Ewa Narożańska,Bogna Brockhuis,Michał Korostyński,Jarosław Sławek,Cezary Zekanowski

Bioengineered 12:9753-9765 PubMed34696681

2021

Long non-coding RNA TRPM2-AS regulates microRNA miR-138-5p and PLAU (Plasminogen Activator, Urokinase) to promote the progression of gastric adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Sun,Fang Zhou,Juan Xue,Chunyan Ji,Yinzong Qu,Yuwei Pan

Cell death discovery 7:32 PubMed33574243

2021

PLAU directs conversion of fibroblasts to inflammatory cancer-associated fibroblasts, promoting esophageal squamous cell carcinoma progression via uPAR/Akt/NF-κB/IL8 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lingling Fang,Yun Che,Chaoqi Zhang,Jianbing Huang,Yuanyuan Lei,Zhiliang Lu,Nan Sun,Jie He

Pharmacological research 143:73-85 PubMed30862605

2019

Novel urokinase-plasminogen activator inhibitor SPINK13 inhibits growth and metastasis of hepatocellular carcinoma in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Wei,Yongzhi Lun,Xiaoping Zhou,Shang He,Lijuan Gao,Yan Liu,Zheng He,Baoming Li,Chengbin Wang

OncoTargets and therapy 11:7733-7743 PubMed30464522

2018

MicroRNA-645 targets urokinase plasminogen activator and decreases the invasive growth of MDA-MB-231 triple-negative breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Du Meng,Ming Lei,Yaxuan Han,Dongli Zhao,Xiaozhi Zhang,Yunyi Yang,Rui Liu

Oncology research 27:515-524 PubMed29386086

2018

Upregulation of miR-324-5p Inhibits Proliferation and Invasion of Colorectal Cancer Cells by Targeting ELAVL1.

Applications

Unspecified application

Species

Unspecified reactive species

Chijiang Gu,Mingyuan Zhang,Weiliang Sun,Changzheng Dong

Scientific reports 7:13570 PubMed29051577

2017

De novo mutations of TUBA3D are associated with keratoconus.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Dan Hao,Peng Chen,Yang-Yang Zhang,Su-Xia Li,Wei-Yun Shi,Hua Gao

Oncology letters 14:7288-7296 PubMed29344165

2017

Overexpression of forkhead box M1 and urokinase-type plasminogen activator in gastric cancer is associated with cancer progression and poor prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Ma,Guangwei Qi,Ji Xu,Haibing Ni,Wulin Xu,Guoqing Ru,Zhongsheng Zhao,Wenjuan Xu,Xujun He

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-uPA antibody [EP6274(2)]

Immunocytochemistry/ Immunofluorescence - Anti-uPA antibody [EP6274(2)] (AB169754)

Western blot - Anti-uPA antibody [EP6274(2)] (AB169754)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (14)

Product promise