Rabbit Recombinant Monoclonal uPA Receptor/U-PAR antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 5 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-uPA Receptor/U-PAR antibody [EPR20191] (AB218106), expandable thumbnail](https://content.abcam.com/products/images/upa-receptor-u-par-antibody-epr20191-ab218106--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img124522.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-uPA Receptor/U-PAR antibody [EPR20191] (AB218106)")
![Western blot - Anti-uPA Receptor/U-PAR antibody [EPR20191] (AB218106), expandable thumbnail](https://content.abcam.com/products/images/upa-receptor-u-par-antibody-epr20191-ab218106--western-blot-img19308.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-uPA Receptor/U-PAR antibody [EPR20191] (AB218106)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-uPA Receptor/U-PAR antibody [EPR20191] (AB218106), expandable thumbnail](https://content.abcam.com/products/images/upa-receptor-u-par-antibody-epr20191-ab218106--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img45765.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-uPA Receptor/U-PAR antibody [EPR20191] (AB218106)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-uPA Receptor/U-PAR antibody [EPR20191] (AB218106), expandable thumbnail](https://content.abcam.com/products/images/upa-receptor-u-par-antibody-epr20191-ab218106--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118640.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-uPA Receptor/U-PAR antibody [EPR20191] (AB218106)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-uPA Receptor/U-PAR antibody [EPR20191] (AB218106), expandable thumbnail](https://content.abcam.com/products/images/upa-receptor-u-par-antibody-epr20191-ab218106--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img26254.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-uPA Receptor/U-PAR antibody [EPR20191] (AB218106)")
Publications
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- Cell stress & chaperones 28:253-2632023ENPP2 alleviates hypoxia/reoxygenation injury and ferroptosis by regulating oxidative stress and mitochondrial function in human cardiac microvascular endothelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuanhua Fang,Yanming Shen,Dongshan LiaoPubMed 37052764
- Frontiers in oncology 12:8627232022A Novel TAF-Related Signature Based on ECM Remodeling Genes Predicts Glioma Prognosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLin-Jian Wang,Peipei Lv,Yongli LouPubMed 35574375
- Frontiers in oncology 11:6664462021Morphine Suppresses Liver Cancer Cell Tumor Properties and .Applications:Unspecified applicationReactive species:Unspecified reactive speciesHao-Wen Zhang,Fei Wang,Ya-Qun Zhou,San-Ping Xu,Shi-Ying Yu,Zhan-Guo ZhangPubMed 33968773
- Journal of cancer research and clinical oncology 146:2189-22032020Prostaglandin E2 receptor 3 (EP3) signaling promotes migration of cervical cancer via urokinase-type plasminogen activator receptor (uPAR).Applications:Unspecified applicationReactive species:Unspecified reactive speciesYao Ye et. al.PubMed 32488496
- Journal of experimental & clinical cancer research 38:2142019GPAA1 promotes gastric cancer progression via upregulation of GPI-anchored protein and enhancement of ERBB signalling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiao-Xin Zhang et. al.PubMed 31118109
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
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Target data
Function
Acts as a receptor for urokinase plasminogen activator (PubMed:15677461). Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form.
Alternative names
CD87, MO3, UPAR, PLAUR, Urokinase plasminogen activator surface receptor, U-PAR, uPAR, Monocyte activation antigen Mo3
Recommended products
Rabbit Recombinant Monoclonal uPA Receptor/U-PAR antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 5 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR20191
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-uPA Receptor/U-PAR antibody [EPR20191] (ab218106), expandable thumbnail](https://content.abcam.com/products/images/upa-receptor-u-par-antibody-epr20191-ab218106--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img124522.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-uPA Receptor/U-PAR antibody [EPR20191] (ab218106)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-uPA Receptor/U-PAR antibody [EPR20191] (ab218106)
Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue labeling uPA Receptor/U-PAR with ab218106 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Membranous staining on dissociative cancer cells in blood vessel of human ovarian cancer is observed [PMID: 25120753].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Western blot - Anti-uPA Receptor/U-PAR antibody [EPR20191] (ab218106), expandable thumbnail](https://content.abcam.com/products/images/upa-receptor-u-par-antibody-epr20191-ab218106--western-blot-img19308.jpg "Western blot - Anti-uPA Receptor/U-PAR antibody [EPR20191] (ab218106)")
Western blot - Anti-uPA Receptor/U-PAR antibody [EPR20191] (ab218106)
Blocking/Dilution buffer: 5% NFDM/TBST.
PMA-stimulated U937 cells express uPA Receptor/U-PAR in the range of 35kDa-60kDa. Non-stimulated U937 cells are negative. The deglycosylated form of the protein is at 35 kDa and the highly glycosylated protein is at 60kDa (PMID:24999729).
All lanes: Western blot - Anti-uPA Receptor/U-PAR antibody [EPR20191] (ab218106) at 1/1000 dilution
Lane 1: Untreated U937 (Human histiocytic lymphoma cell line) whole cell lysate at 10 µg
Lane 2: U937 whole cell lysate treated with 200nM PMA (Phorbol-12-myristate-13-acetate) for 72 hours at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 37 kDa
Observed band size: 35-65 kDa
Exposure time: 15s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-uPA Receptor/U-PAR antibody [EPR20191] (ab218106), expandable thumbnail](https://content.abcam.com/products/images/upa-receptor-u-par-antibody-epr20191-ab218106--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img45765.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-uPA Receptor/U-PAR antibody [EPR20191] (ab218106)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-uPA Receptor/U-PAR antibody [EPR20191] (ab218106)
Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue labeling uPA Receptor/U-PAR with ab218106 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Membranous staining on the macrophages of human ovarian cancer is observed [PMID: 25575713].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-uPA Receptor/U-PAR antibody [EPR20191] (ab218106), expandable thumbnail](https://content.abcam.com/products/images/upa-receptor-u-par-antibody-epr20191-ab218106--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118640.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-uPA Receptor/U-PAR antibody [EPR20191] (ab218106)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-uPA Receptor/U-PAR antibody [EPR20191] (ab218106)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue labeling uPA Receptor/U-PAR with ab218106 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Cytoplasmic staining on the stromal cells of human lung cancer is observed [PMID: 25575713].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-uPA Receptor/U-PAR antibody [EPR20191] (ab218106), expandable thumbnail](https://content.abcam.com/products/images/upa-receptor-u-par-antibody-epr20191-ab218106--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img26254.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-uPA Receptor/U-PAR antibody [EPR20191] (ab218106)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-uPA Receptor/U-PAR antibody [EPR20191] (ab218106)
Immunohistochemical analysis of paraffin-embedded human artery tissue labeling uPA Receptor/U-PAR with ab218106 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Positive staining on the neutrophils in human artery is observed [PMID: 25575713].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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