Rabbit Recombinant Monoclonal uPA Receptor/U-PAR antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-uPA Receptor/U-PAR antibody [EPR20191] - BSA and Azide free (AB251557), expandable thumbnail](https://content.abcam.com/products/images/upa-receptor-u-par-antibody-epr20191-bsa-and-azide-free-ab251557--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img124522.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-uPA Receptor/U-PAR antibody [EPR20191] - BSA and Azide free (AB251557)")
![Western blot - Anti-uPA Receptor/U-PAR antibody [EPR20191] - BSA and Azide free (AB251557), expandable thumbnail](https://content.abcam.com/products/images/upa-receptor-u-par-antibody-epr20191-bsa-and-azide-free-ab251557--western-blot-img19308.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-uPA Receptor/U-PAR antibody [EPR20191] - BSA and Azide free (AB251557)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-uPA Receptor/U-PAR antibody [EPR20191] - BSA and Azide free (AB251557), expandable thumbnail](https://content.abcam.com/products/images/upa-receptor-u-par-antibody-epr20191-bsa-and-azide-free-ab251557--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img45765.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-uPA Receptor/U-PAR antibody [EPR20191] - BSA and Azide free (AB251557)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-uPA Receptor/U-PAR antibody [EPR20191] - BSA and Azide free (AB251557), expandable thumbnail](https://content.abcam.com/products/images/upa-receptor-u-par-antibody-epr20191-bsa-and-azide-free-ab251557--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118640.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-uPA Receptor/U-PAR antibody [EPR20191] - BSA and Azide free (AB251557)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-uPA Receptor/U-PAR antibody [EPR20191] - BSA and Azide free (AB251557), expandable thumbnail](https://content.abcam.com/products/images/upa-receptor-u-par-antibody-epr20191-bsa-and-azide-free-ab251557--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img26254.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-uPA Receptor/U-PAR antibody [EPR20191] - BSA and Azide free (AB251557)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Acts as a receptor for urokinase plasminogen activator (PubMed:15677461). Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form.
Alternative names
CD87, MO3, UPAR, PLAUR, Urokinase plasminogen activator surface receptor, U-PAR, uPAR, Monocyte activation antigen Mo3
Recommended products
Rabbit Recombinant Monoclonal uPA Receptor/U-PAR antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR20191
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab251557 is the carrier-free version of Anti-uPA Receptor/U-PAR antibody [EPR20191] ab218106.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-uPA Receptor/U-PAR antibody [EPR20191] - BSA and Azide free (ab251557), expandable thumbnail](https://content.abcam.com/products/images/upa-receptor-u-par-antibody-epr20191-bsa-and-azide-free-ab251557--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img124522.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-uPA Receptor/U-PAR antibody [EPR20191] - BSA and Azide free (ab251557)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-uPA Receptor/U-PAR antibody [EPR20191] - BSA and Azide free (ab251557)
This data was developed using Anti-uPA Receptor/U-PAR antibody [EPR20191] ab218106, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue labeling uPA Receptor/U-PAR with Anti-uPA Receptor/U-PAR antibody [EPR20191] ab218106 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on dissociative cancer cells in blood vessel of human ovarian cancer is observed [PMID: 25120753]. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Western blot - Anti-uPA Receptor/U-PAR antibody [EPR20191] - BSA and Azide free (ab251557), expandable thumbnail](https://content.abcam.com/products/images/upa-receptor-u-par-antibody-epr20191-bsa-and-azide-free-ab251557--western-blot-img19308.jpg "Western blot - Anti-uPA Receptor/U-PAR antibody [EPR20191] - BSA and Azide free (ab251557)")
Western blot - Anti-uPA Receptor/U-PAR antibody [EPR20191] - BSA and Azide free (ab251557)
This data was developed using Anti-uPA Receptor/U-PAR antibody [EPR20191] ab218106, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
PMA-stimulated U937 cells express uPA Receptor/U-PAR in the range of 35kDa-60kDa. Non-stimulated U937 cells are negative. The deglycosylated form of the protein is at 35 kDa and the highly glycosylated protein is at 60kDa (PMID: 24999729).
All lanes: Western blot - Anti-uPA Receptor/U-PAR antibody [EPR20191] (Anti-uPA Receptor/U-PAR antibody [EPR20191] ab218106) at 1/1000 dilution
Lane 1: Untreated U937 (Human histiocytic lymphoma cell line) whole cell lysate at 10 µg
Lane 2: U937 whole cell lysate treated with 200nM PMA (Phorbol-12-myristate-13-acetate) for 72 hours at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 37 kDa
Observed band size: 35-65 kDa
Exposure time: 15s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-uPA Receptor/U-PAR antibody [EPR20191] - BSA and Azide free (ab251557), expandable thumbnail](https://content.abcam.com/products/images/upa-receptor-u-par-antibody-epr20191-bsa-and-azide-free-ab251557--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img45765.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-uPA Receptor/U-PAR antibody [EPR20191] - BSA and Azide free (ab251557)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-uPA Receptor/U-PAR antibody [EPR20191] - BSA and Azide free (ab251557)
This data was developed using Anti-uPA Receptor/U-PAR antibody [EPR20191] ab218106, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue labeling uPA Receptor/U-PAR with Anti-uPA Receptor/U-PAR antibody [EPR20191] ab218106 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on the macrophages of human ovarian cancer is observed [PMID: 25575713]. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-uPA Receptor/U-PAR antibody [EPR20191] - BSA and Azide free (ab251557), expandable thumbnail](https://content.abcam.com/products/images/upa-receptor-u-par-antibody-epr20191-bsa-and-azide-free-ab251557--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118640.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-uPA Receptor/U-PAR antibody [EPR20191] - BSA and Azide free (ab251557)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-uPA Receptor/U-PAR antibody [EPR20191] - BSA and Azide free (ab251557)
This data was developed using Anti-uPA Receptor/U-PAR antibody [EPR20191] ab218106, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human lung cancer tissue labeling uPA Receptor/U-PAR with Anti-uPA Receptor/U-PAR antibody [EPR20191] ab218106 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on the stromal cells of human lung cancer is observed [PMID: 25575713]. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-uPA Receptor/U-PAR antibody [EPR20191] - BSA and Azide free (ab251557), expandable thumbnail](https://content.abcam.com/products/images/upa-receptor-u-par-antibody-epr20191-bsa-and-azide-free-ab251557--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img26254.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-uPA Receptor/U-PAR antibody [EPR20191] - BSA and Azide free (ab251557)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-uPA Receptor/U-PAR antibody [EPR20191] - BSA and Azide free (ab251557)
This data was developed using Anti-uPA Receptor/U-PAR antibody [EPR20191] ab218106, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human artery tissue labeling uPA Receptor/U-PAR with Anti-uPA Receptor/U-PAR antibody [EPR20191] ab218106 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on the neutrophils in human artery is observed [PMID: 25575713]. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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