AB255815

Anti-uPA Receptor/U-PAR antibody [EPR22814-273]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal uPA Receptor/U-PAR antibody. Suitable for IP, Flow Cyt, WB and reacts with Mouse samples. Cited in 1 publication.

View Alternative Names

CD87, Urokinase plasminogen activator surface receptor, U-PAR, uPAR, Plaur

4 Images

Flow Cytometry - Anti-uPA Receptor/U-PAR antibody [EPR22814-273] (AB255815)

Flow Cyt

Unknown

Flow Cytometry - Anti-uPA Receptor/U-PAR antibody [EPR22814-273] (AB255815)

Flow Cytometric analysis of mouse PBMC (Mouse primary peripheral blood mononuclear cell) treated with 100ng/ml LPS for 16h, labeling uPA Receptor/U-PAR using ab255815 at a 1/600 dilution. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary at a 1/2000 dilution.

Cells were stained with rabbit IgG (Left) or ab255815 (Right). Then stained with anti-CD11b conjugated to Pacific blue.

Gated on viable cells.

Expression of uPAR on monocytes and granulocytes are observed in LPS-treated PBMC (PMID : 11447203).

Unknown

Immunoprecipitation - Anti-uPA Receptor/U-PAR antibody [EPR22814-273] (AB255815)

uPA Receptor/U-PAR was immunoprecipitated from 0.35 mg LLC1 (mouse lung carcinoma) whole cell lysate 10μg with ab255815 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab255815 at a 1/1000 dilution (0.59 μg/ml). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at a 1/5000 dilution.

Lane 1 : LLC1 (mouse lung carcinoma) whole cell lysate 10μg.

Lane 2 : ab255815 IP in LLC1 whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab255815 in LLC1 whole cell lysate.

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : 3 seconds.

All lanes:

Immunoprecipitation - Anti-uPA Receptor/U-PAR antibody [EPR22814-273] (ab255815)

Predicted band size: 37 kDa

false

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Western blot - Anti-uPA Receptor/U-PAR antibody [EPR22814-273] (AB255815)

uPAR is a glycoprotein. Full-length GPI-anchored uPAR (60kDa) and truncated soluble uPAR (45kDa) are observed. The molecular weight observed is consistent with what has been described in the literature (PMID : 19667118, PMID 24999729, PMID : 11535128).

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure times : Lane 1 : 81 secs; Lane 2 : 158 secs.

All lanes:

Western blot - Anti-uPA Receptor/U-PAR antibody [EPR22814-273] (ab255815) at 1/1000 dilution

Lane 1:

Mouse lung tissue lysate at 20 µg

Lane 2:

LLC1 (mouse lung carcinoma) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 37 kDa

false

Lab

Western blot - Anti-uPA Receptor/U-PAR antibody [EPR22814-273] (AB255815)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression tissue : kidney and spleen.

All lanes:

Western blot - Anti-uPA Receptor/U-PAR antibody [EPR22814-273] (ab255815) at 1/1000 dilution

Lane 1:

Mouse lung tissue lysate at 20 µg

Lane 2:

Mouse kidney tissue lysate at 20 µg

Lane 3:

Mouse spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 37 kDa

Observed band size: 65 kDa,37 kDa

false

Exposure time: 180s

Carrier free

Anti-uPA Receptor/U-PAR antibody [EPR22814-273] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22814-273

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

WB, IP, Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/600", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA.

See full target information Plaur

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 7:1380 PubMed39443661

2024

Deficiencies in corin and atrial natriuretic peptide-mediated signaling impair endochondral ossification in bone development.

Applications

Unspecified application

Species

Unspecified reactive species

Zibin Zhou,Xiaoyu Mao,Chun Jiang,Wenguo Li,Tiantian Zhou,Meng Liu,Shijin Sun,Mengting Wang,Ningzheng Dong,Qingyu Wu,Haibin Zhou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-uPA Receptor/U-PAR antibody [EPR22814-273]

Flow Cytometry - Anti-uPA Receptor/U-PAR antibody [EPR22814-273] (AB255815)

Immunoprecipitation - Anti-uPA Receptor/U-PAR antibody [EPR22814-273] (AB255815)

Western blot - Anti-uPA Receptor/U-PAR antibody [EPR22814-273] (AB255815)

Western blot - Anti-uPA Receptor/U-PAR antibody [EPR22814-273] (AB255815)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

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