Anti-uPA Receptor/U-PAR antibody [EPR22814-273] - BSA and Azide free

• Flow Cyt

Unknown

Flow Cytometry - Anti-uPA Receptor/U-PAR antibody [EPR22814-273] - BSA and Azide free (AB256763)

Flow Cytometric analysis of mouse PBMC (Mouse primary peripheral blood mononuclear cell) treated with 100ng/ml LPS for 16h, labeling uPA Receptor/U-PAR using ab255815 at a 1/600 dilution. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary at a 1/2000 dilution.

Cells were stained with rabbit IgG (Left) or ab255815 (Right). Then stained with anti-CD11b conjugated to Pacific blue.

Gated on viable cells.

Expression of uPAR on monocytes and granulocytes are observed in LPS-treated PBMC (PMID : 11447203).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255815).

• IP

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Immunoprecipitation - Anti-uPA Receptor/U-PAR antibody [EPR22814-273] - BSA and Azide free (AB256763)

uPA Receptor/U-PAR was immunoprecipitated from 0.35 mg LLC1 (mouse lung carcinoma) whole cell lysate 10μg with ab255815 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab255815 at a 1/1000 dilution (0.59 μg/ml). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as at a 1/5000 dilution.

Lane 1 : LLC1 (mouse lung carcinoma) whole cell lysate 10μg.

Lane 2 : ab255815 IP in LLC1 whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab255815 in LLC1 whole cell lysate.

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : 3 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255815).

All lanes:

Immunoprecipitation - Anti-uPA Receptor/U-PAR antibody [EPR22814-273] (<a href='/en-us/products/primary-antibodies/upa-receptor-u-par-antibody-epr22814-273-ab255815'>ab255815</a>)

Predicted band size: 37 kDa

false

• WB

Lab

Western blot - Anti-uPA Receptor/U-PAR antibody [EPR22814-273] - BSA and Azide free (AB256763)

This data was developed using ab255815, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression tissue : kidney and spleen.

All lanes:

Western blot - Anti-uPA Receptor/U-PAR antibody [EPR22814-273] (<a href='/en-us/products/primary-antibodies/upa-receptor-u-par-antibody-epr22814-273-ab255815'>ab255815</a>) at 1/1000 dilution

Lane 1:

Mouse lung tissue lysate at 20 µg

Lane 2:

Mouse kidney tissue lysate at 20 µg

Lane 3:

Mouse spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 37 kDa

Observed band size: 65 kDa,37 kDa

false

Exposure time: 180s