Anti-uPA Receptor/U-PAR antibody [EPR26916-73] is a Rabbit Monoclonal antibody that is used in uPA Receptor/U-PAR IHC-P, IP, Western Blot. Suitable for Mouse samples.
uPAR has been identified as a universal marker of senescent cells and an effective therapeutic target to eliminate senescent cells that promote tumor progression and evade standard anti-cancer drugs that target dividing cells. CAR-T cells that target uPAR extend the survival of mice with lung adenocarcinoma that are treated with a senescence-inducing combination of drugs. uPAR is also required for the pro-angiogenic activity of melanoma exosomes.
Images
![Western blot - Anti-uPA Receptor/U-PAR antibody [EPR26916-73] (AB307895), expandable thumbnail](https://content.abcam.com/products/images/upa-receptor-u-par-antibody-epr26916-73-ab307895--western-blot-img445040.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-uPA Receptor/U-PAR antibody [EPR26916-73] (AB307895)")
![Western blot - Anti-uPA Receptor/U-PAR antibody [EPR26916-73] (AB307895), expandable thumbnail](https://content.abcam.com/products/images/upa-receptor-u-par-antibody-epr26916-73-ab307895--western-blot-img408485.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-uPA Receptor/U-PAR antibody [EPR26916-73] (AB307895)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-uPA Receptor/U-PAR antibody [EPR26916-73] (AB307895), expandable thumbnail](https://content.abcam.com/products/images/upa-receptor-u-par-antibody-epr26916-73-ab307895--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img408973.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-uPA Receptor/U-PAR antibody [EPR26916-73] (AB307895)")
![Immunohistochemistry - Anti-uPA Receptor/U-PAR antibody [EPR26916-73] (AB307895), expandable thumbnail](https://content.abcam.com/products/images/upa-receptor-u-par-antibody-epr26916-73-ab307895--immunohistochemistry-img408489.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry - Anti-uPA Receptor/U-PAR antibody [EPR26916-73] (AB307895)")
![Western blot - Anti-uPA Receptor/U-PAR antibody [EPR26916-73] (AB307895), expandable thumbnail](https://content.abcam.com/products/images/upa-receptor-u-par-antibody-epr26916-73-ab307895--western-blot-img408488.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-uPA Receptor/U-PAR antibody [EPR26916-73] (AB307895)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| WB | IHC-P | IP | ICC/IF | Flow Cyt | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Tested | Tested | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/30 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Target data
Function
Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA.
Alternative names
CD87, Urokinase plasminogen activator surface receptor, U-PAR, uPAR, Plaur
Recommended products
Anti-uPA Receptor/U-PAR antibody [EPR26916-73] is a Rabbit Monoclonal antibody that is used in uPA Receptor/U-PAR IHC-P, IP, Western Blot. Suitable for Mouse samples.
uPAR has been identified as a universal marker of senescent cells and an effective therapeutic target to eliminate senescent cells that promote tumor progression and evade standard anti-cancer drugs that target dividing cells. CAR-T cells that target uPAR extend the survival of mice with lung adenocarcinoma that are treated with a senescence-inducing combination of drugs. uPAR is also required for the pro-angiogenic activity of melanoma exosomes.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR26916-73
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-uPA Receptor/U-PAR antibody [EPR26916-73] (ab307895), expandable thumbnail](https://content.abcam.com/products/images/upa-receptor-u-par-antibody-epr26916-73-ab307895--western-blot-img445040.jpg "Western blot - Anti-uPA Receptor/U-PAR antibody [EPR26916-73] (ab307895)")
Western blot - Anti-uPA Receptor/U-PAR antibody [EPR26916-73] (ab307895)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression tissue: kidney and spleen.
All lanes: Western blot - Anti-uPA Receptor/U-PAR antibody [EPR26916-73] (ab307895) at 1/1000 dilution
Lane 1: Mouse lung tissue lysate at 20 µg
Lane 2: Mouse kidney tissue lysate at 20 µg
Lane 3: Mouse spleen tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 37 kDa
Observed band size: 37 kDa, 65 kDa
Exposure time: 180s
![Western blot - Anti-uPA Receptor/U-PAR antibody [EPR26916-73] (ab307895), expandable thumbnail](https://content.abcam.com/products/images/upa-receptor-u-par-antibody-epr26916-73-ab307895--western-blot-img408485.jpg "Western blot - Anti-uPA Receptor/U-PAR antibody [EPR26916-73] (ab307895)")
Western blot - Anti-uPA Receptor/U-PAR antibody [EPR26916-73] (ab307895)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
All lanes: Western blot - Anti-uPA Receptor/U-PAR antibody [EPR26916-73] (ab307895) at 1/1000 dilution
Lane 1: Mouse lung tissue lysate at 40 µg
Lane 2: Mouse kidney tissue lysate at 40 µg
Lane 3: Mouse spleen tissue lysate at 40 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Developed using the ECL technique.
Observed band size: 37 kDa, 65 kDa
Exposure time: 48s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-uPA Receptor/U-PAR antibody [EPR26916-73] (ab307895), expandable thumbnail](https://content.abcam.com/products/images/upa-receptor-u-par-antibody-epr26916-73-ab307895--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img408973.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-uPA Receptor/U-PAR antibody [EPR26916-73] (ab307895)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-uPA Receptor/U-PAR antibody [EPR26916-73] (ab307895)
Immunohistochemical analysis of paraffin-embedded mouse head and neck squamous cell carcinoma tissue labeling uPA Receptor/U-PAR with ab307895 at 1/100 followed by ready to use LeicaDS9800 (Bond™, Polymer Refine Detection). Positive staining on the mouse head and neck squamous cell carcinoma. The section was incubated with ab307895 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™, Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.![Immunohistochemistry - Anti-uPA Receptor/U-PAR antibody [EPR26916-73] (ab307895), expandable thumbnail](https://content.abcam.com/products/images/upa-receptor-u-par-antibody-epr26916-73-ab307895--immunohistochemistry-img408489.jpg "Immunohistochemistry - Anti-uPA Receptor/U-PAR antibody [EPR26916-73] (ab307895)")
Immunohistochemistry - Anti-uPA Receptor/U-PAR antibody [EPR26916-73] (ab307895)
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling uPA Receptor/U-PAR with ab307895 at 1/100 followed by ready to use LeicaDS9800 (Bond™, Polymer Refine Detection). Positive staining on the perifollicular zone of mouse spleen (PMID: 11156692). The section was incubated with ab307895 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™, Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.![Western blot - Anti-uPA Receptor/U-PAR antibody [EPR26916-73] (ab307895), expandable thumbnail](https://content.abcam.com/products/images/upa-receptor-u-par-antibody-epr26916-73-ab307895--western-blot-img408488.jpg "Western blot - Anti-uPA Receptor/U-PAR antibody [EPR26916-73] (ab307895)")
Western blot - Anti-uPA Receptor/U-PAR antibody [EPR26916-73] (ab307895)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 19667118).
Observed MW(kDa) 24 (deglycosylated form), 37-65(glycosylated form).
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: Lane 1-3: 6 seconds, Lane4: 37 seconds.All lanes: Western blot - Anti-uPA Receptor/U-PAR antibody [EPR26916-73] (ab307895) at 1/1000 dilution
Lane 1: Untreated bEnd.3 whole cell lysate at 30 µg
Lane 2: bEnd.3 whole cell lysate treated with Protein Deglycosylation MIX II at 30 µg
Lane 3: RAW264.7 whole cell lysate at 40 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 24 kDa, 37 kDa, 65 kDa
Exposure time: 6s
![Immunohistochemistry - Anti-uPA Receptor/U-PAR antibody [EPR26916-73] (ab307895), expandable thumbnail](https://content.abcam.com/products/images/upa-receptor-u-par-antibody-epr26916-73-ab307895--immunohistochemistry-img408487.jpg "Immunohistochemistry - Anti-uPA Receptor/U-PAR antibody [EPR26916-73] (ab307895)")
Immunohistochemistry - Anti-uPA Receptor/U-PAR antibody [EPR26916-73] (ab307895)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling uPA Receptor/U-PAR with ab307895 at 1/100 followed by ready to use LeicaDS9800 (Bond™, Polymer Refine Detection). Positive staining on glomeruli and the endothelial cells of mouse kidney (PMID: 11156692). The section was incubated with ab307895 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™, Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.![Immunoprecipitation - Anti-uPA Receptor/U-PAR antibody [EPR26916-73] (ab307895), expandable thumbnail](https://content.abcam.com/products/images/upa-receptor-u-par-antibody-epr26916-73-ab307895--immunoprecipitation-img408486.jpg "Immunoprecipitation - Anti-uPA Receptor/U-PAR antibody [EPR26916-73] (ab307895)")
Immunoprecipitation - Anti-uPA Receptor/U-PAR antibody [EPR26916-73] (ab307895)
uPA Receptor/U-PAR was immunoprecipitated from 0.35 mg bEnd.3 (mouse brain endothelial cell) whole cell lysate with ab307895 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307895 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: bEnd.3 whole cell lysate
Lane 2: ab307895 IP in bEnd.3 whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab307895 in bEnd.3 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 8 seconds.All lanes: Immunoprecipitation - Anti-uPA Receptor/U-PAR antibody [EPR26916-73] (ab307895) at 1/30 dilution
All lanes: bEnd.3 whole cell lysate at 10 µg
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Observed band size: 37 kDa, 65 kDa
Exposure time: 8s
![Immunohistochemistry - Anti-uPA Receptor/U-PAR antibody [EPR26916-73] (ab307895), expandable thumbnail](https://content.abcam.com/products/images/upa-receptor-u-par-antibody-epr26916-73-ab307895--immunohistochemistry-img408487.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry - Anti-uPA Receptor/U-PAR antibody [EPR26916-73] (ab307895)")
![Immunoprecipitation - Anti-uPA Receptor/U-PAR antibody [EPR26916-73] (ab307895), expandable thumbnail](https://content.abcam.com/products/images/upa-receptor-u-par-antibody-epr26916-73-ab307895--immunoprecipitation-img408486.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-uPA Receptor/U-PAR antibody [EPR26916-73] (ab307895)")
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