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AB196562

Anti-UPD antibody

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(2 Publications)

Rabbit Polyclonal UPD antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human UROD.

View Alternative Names

Uroporphyrinogen decarboxylase, UPD, URO-D, UROD

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-UPD antibody (AB196562)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-UPD antibody (AB196562)

Immunofluorescent analysis of U20S cells labeling UPD (red) with ab196562 at 1/50 dilution. Nuclei were counterstained with DAPI (blue).

Western blot - Anti-UPD antibody (AB196562)
  • WB

Supplier Data

Western blot - Anti-UPD antibody (AB196562)

All lanes:

Western blot - Anti-UPD antibody (ab196562) at 1/500 dilution

Lane 1:

SW620 cell extract

Lane 2:

K562 cell extract

Lane 3:

SW480 cell extract

Lane 4:

Mouse liver extract

Lane 5:

Mouse kidney extract

Lane 6:

Mouse testis extract

Lane 7:

Mouse heart extract

Lane 8:

HepG2 cell extract

Predicted band size: 41 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human UROD.

P06132

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ubiquitously expressed UDP-glucuronosyltransferase protein family often referred to as UGTs plays essential roles in the detoxification and metabolism of both endogenous and exogenous compounds. Individual UGT enzymes convert lipophilic molecules into more water-soluble glucuronides facilitating their excretion. This process involves the transfer of glucuronic acid from uridine diphosphate glucuronic acid (UDPGA) to substrates. UGTs display diverse tissue expression with significant activity in the liver gastrointestinal tract and kidneys. These enzymes typically have a molecular mass of around 50-60 kDa.
Biological function summary

UDP-glucuronosyltransferases are integral to phase II metabolism transforming small hydrophobic molecules for elimination. The UGT family functions within the microsomal enzyme system and associates with lipid membranes in the endoplasmic reticulum. UGTs work as monomers or form enzyme complexes particularly with other phase II enzymes such as sulfotransferases to efficiently manage the body's biochemical landscape. They recognize a wide range of substrates including hormones bile acids and drugs highlighting their metabolic versatility.

Pathways

UDP-glucuronosyltransferases participate primarily in the detoxification pathway. This pathway relies on UGTs for the conjugation of xenobiotics aiding in detoxifying harmful compounds. The enzymes also integrate into the bilirubin metabolic pathway where they catalyze the conjugation of bilirubin reducing its toxicity. Notably UGT1A1 a member of the UGT family takes part in this pathway and interacts with other proteins such as cytochrome P450 enzymes which are important in drug metabolism and synthesis of cholesterol steroids and other lipids.

Defects or deficiencies in UDP-glucuronosyltransferases can lead to clinical conditions like Gilbert's syndrome and Crigler-Najjar syndrome. Gilbert's syndrome results from mutations in UGT1A1 causing mild unconjugated hyperbilirubinemia. Crigler-Najjar syndrome a more severe disorder arises from significantly impaired UGT1A1 function leading to extreme cases of hyperbilirubinemia that may require liver transplantation. The interrelationship between UGT1A1 and proteins like albumin which binds bilirubin in the bloodstream highlights the important impact of glucuronidation in maintaining bilirubin homeostasis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the sequential decarboxylation of the four acetate side chains of uroporphyrinogen to form coproporphyrinogen and participates in the fifth step in the heme biosynthetic pathway (PubMed : 11069625, PubMed : 11719352, PubMed : 14633982, PubMed : 18004775, PubMed : 21668429). Isomer I or isomer III of uroporphyrinogen may serve as substrate, but only coproporphyrinogen III can ultimately be converted to heme (PubMed : 11069625, PubMed : 11719352, PubMed : 14633982, PubMed : 21668429). In vitro also decarboxylates pentacarboxylate porphyrinogen I (PubMed : 12071824).
See full target information UROD
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature cancer 1:533-545 PubMed32984844

2020

eIF5B drives integrated stress response-dependent translation of PD-L1 in lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shruthy Suresh,BeiBei Chen,Jingfei Zhu,Ryan J Golden,Changzheng Lu,Bret M Evers,Nicole Novaresi,Bethany Smith,Xiaowei Zhan,Vanessa Schmid,Sojeong Jun,Chelsea M Karacz,Michael Peyton,Lin Zhong,Zhuoyu Wen,Adwait Amod Sathe,Chao Xing,Carmen Behrens,Ignacio I Wistuba,Guanghua Xiao,Yang Xie,Yang-Xin Fu,John D Minna,Joshua T Mendell,Kathryn A O'Donnell

Life science alliance 3: PubMed32071282

2020

The iron-sulfur helicase DDX11 promotes the generation of single-stranded DNA for CHK1 activation.

Applications

Unspecified application

Species

Unspecified reactive species

Anna K Simon,Sandra Kummer,Sebastian Wild,Aleksandra Lezaja,Federico Teloni,Stanislaw K Jozwiakowski,Matthias Altmeyer,Kerstin Gari
View all publications
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Product promise

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