AB134566

Anti-UPF3B/RENT3B antibody

Name: Anti-UPF3B/RENT3B antibody (ab134566) | Abcam
Brand: Abcam Limited
SKU: AB134566
Price: 535 USD
Availability: InStock

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(6 Publications)

Rabbit Polyclonal UPF3B/RENT3B antibody. Suitable for IP, WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human UPF3B aa 300-350.

View Alternative Names

RENT3B, UPF3X, UPF3B, Regulator of nonsense transcripts 3B, Nonsense mRNA reducing factor 3B, Up-frameshift suppressor 3 homolog B, Up-frameshift suppressor 3 homolog on chromosome X, hUpf3B, hUpf3p-X

2 Images

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Immunoprecipitation - Anti-UPF3B/RENT3B antibody (AB134566)

Detection of UPF3B/RENT3B by Western Blot of Immunprecipitate.
Immunoprecipitation of HeLa whole cell lysate using ab134566 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane) followed by detection of UPF3B/RENT3B with ab134566 at 1µg/ml. The lysate in lane 2 is precipitated with an IgG control antibody. Detection : Chemiluminescence with exposure time of 3 seconds.

All lanes:

Immunoprecipitation - Anti-UPF3B/RENT3B antibody (ab134566)

Predicted band size: 58 kDa

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Western blot - Anti-UPF3B/RENT3B antibody (AB134566)

All lanes:

Western blot - Anti-UPF3B/RENT3B antibody (ab134566) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

293T whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Predicted band size: 58 kDa

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Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human UPF3B aa 300-350. The exact immunogen used to generate this antibody is proprietary information.

Q9BZI7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Bat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UPF3B also known as RENT3B is a critical component of the nonsense-mediated mRNA decay (NMD) pathway which surveils and degrades mRNA transcripts with premature stop codons. UPF3B weighs approximately 54 kDa and primarily resides in the nucleus but can shuttle to the cytoplasm. Expression of UPF3B is notably prevalent in neuronal tissues suggesting a specialized role in brain function and development.

Biological function summary

UPF3B interacts with other proteins within the NMD complex to regulate mRNA stability and translation efficiency. As part of this complex UPF3B serves as a link between the nuclear and cytoplasmic phases of NMD. It interacts with UPF2 and UPF1 acting as a bridge to initiate downstream degradation processes. Through these interactions UPF3B balances gene expression and prevents the production of potentially harmful truncated proteins.

Pathways

UPF3B plays an important role in modulating the NMD pathway which is an important post-transcriptional regulatory mechanism in cells. UPF3B works alongside proteins like SMG1 and eIF4A3 within this pathway. It participates in the repression of aberrant mRNA and this function relates to RNA surveillance and cell cycle control. By ensuring faulty transcripts are removed UPF3B helps maintain cellular homeostasis and supports accurate gene expression profiles.

UPF3B mutations associate with X-linked intellectual disability (XLID) and schizophrenia. UPF3B's connection to XLID links it to the regulation of essential neurological functions possibly through its interaction with proteins like UPF2 and eIF4A3. These interactions indicate that proper functioning of NMD components including UPF3B is necessary for normal brain development and cognitive processes. Dysregulation or mutation of UPF3B can impact neural gene regulation leading to serious cognitive and mental health issues.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC) and serving as link between the EJC core and NMD machinery. Recruits UPF2 at the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2-UPF3 surveillance complex (including UPF1 bound to release factors at the stalled ribosome) is believed to activate NMD. In cooperation with UPF2 stimulates both ATPase and RNA helicase activities of UPF1. Binds spliced mRNA upstream of exon-exon junctions. In vitro, stimulates translation; the function is independent of association with UPF2 and components of the EJC core.

See full target information UPF3B

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

RNA (New York, N.Y.) 31:1872-1885 PubMed41057268

2025

UPF1 shuttles between nucleus and cytoplasm independently of its RNA-binding and ATPase activities.

Applications

Unspecified application

Species

Unspecified reactive species

Sofia Nasif,Andrea Brigitte Eberle,Karin Schranz,Remo Hadorn,Sutapa Chakrabarti,Oliver Mühlemann

Cancer science 115:2646-2658 PubMed38889220

2024

Unveiling the role of UPF3B in hepatocellular carcinoma: Potential therapeutic target.

Applications

Unspecified application

Species

Unspecified reactive species

Bowen Hou,Min Shu,Chenghao Liu,Yunfeng Du,Cuicui Xu,Huijiao Jiang,Jun Hou,Xueling Chen,Lianghai Wang,Xiangwei Wu

Journal of advanced research 68:257-270 PubMed38402949

2024

HnRNPR-mediated UPF3B mRNA splicing drives hepatocellular carcinoma metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Wang,Dong Qian,Jiabei Wang,Yao Liu,Wenguang Luo,Hongyan Zhang,Jingjing Cheng,Heng Li,Yang Wu,Wuhan Li,Jing Wang,Xia Yang,Tianzhi Zhang,Dong Han,Qinyao Wang,Chris Zhiyi Zhang,Lianxin Liu

Molecular cell 82:2779-2796.e10 PubMed35675814

2022

AKT constitutes a signal-promoted alternative exon-junction complex that regulates nonsense-mediated mRNA decay.

Applications

Unspecified application

Species

Unspecified reactive species

Hana Cho,Elizabeth T Abshire,Maximilian W Popp,Christoph Pröschel,Joshua L Schwartz,Gene W Yeo,Lynne E Maquat

Nucleic acids research 49:11022-11037 PubMed34634811

2021

A role for AKT1 in nonsense-mediated mRNA decay.

Applications

Unspecified application

Species

Unspecified reactive species

Martine Palma,Catherine Leroy,Sophie Salomé-Desnoulez,Elisabeth Werkmeister,Rebekah Kong,Marc Mongy,Hervé Le Hir,Fabrice Lejeune

mBio 9: PubMed30401782

2018

The Cellular NMD Pathway Restricts Zika Virus Infection and Is Targeted by the Viral Capsid Protein.

Applications

Unspecified application

Species

Unspecified reactive species

Krystal A Fontaine,Kristoffer E Leon,Mir M Khalid,Sakshi Tomar,David Jimenez-Morales,Mariah Dunlap,Julia A Kaye,Priya S Shah,Steve Finkbeiner,Nevan J Krogan,Melanie Ott

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-UPF3B/RENT3B antibody

Immunoprecipitation - Anti-UPF3B/RENT3B antibody (AB134566)

Western blot - Anti-UPF3B/RENT3B antibody (AB134566)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Complementary Products

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Primary Antibodies

Primary Antibodies

Secondary Antibodies

Product promise