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Rabbit Recombinant Monoclonal REN3A antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human, Zebrafish, Recombinant fragment - Zebrafish samples.

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Images

Western blot - Anti-UPF3B/RENT3B + UPF3A antibody [EPR24112-81] - BSA and Azide free (AB288302), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-UPF3B/RENT3B + UPF3A antibody [EPR24112-81] - BSA and Azide free (AB288302), expandable thumbnail
  • Immunoprecipitation - Anti-UPF3B/RENT3B + UPF3A antibody [EPR24112-81] - BSA and Azide free (AB288302), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-UPF3B/RENT3B + UPF3A antibody [EPR24112-81] - BSA and Azide free (AB288302), expandable thumbnail
  • Western blot - Anti-UPF3B/RENT3B + UPF3A antibody [EPR24112-81] - BSA and Azide free (AB288302), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFIPWBFlow Cyt (Intra)
Human
Not recommended
Tested
Tested
Tested
Tested
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Recombinant fragment - Zebrafish
Not recommended
Not recommended
Not recommended
Tested
Not recommended
Zebrafish
Not recommended
Expected
Expected
Tested
Expected

Not recommended
Not recommended

Species
Rat, Mouse, Human, Zebrafish, Recombinant fragment - Zebrafish
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Zebrafish
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Rat, Mouse, Recombinant fragment - Zebrafish
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Zebrafish
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Rat, Mouse, Recombinant fragment - Zebrafish
Dilution info
-
Notes

-

Tested
Tested

Species
Recombinant fragment - Zebrafish, Human, Zebrafish
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Rat, Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Zebrafish
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Rat, Mouse, Recombinant fragment - Zebrafish
Dilution info
-
Notes

-

Target data

Function

Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC) and serving as link between the EJC core and NMD machinery. Recruits UPF2 at the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2-UPF3 surveillance complex (including UPF1 bound to release factors at the stalled ribosome) is believed to activate NMD. Binds spliced mRNA upstream of exon-exon junctions (By similarity).

Additional Targets

upf3b

Alternative names

si:dkey-21o13.6, upf3a, Regulator of nonsense transcripts 3A, Up-frameshift suppressor 3 homolog A

Recommended products

Rabbit Recombinant Monoclonal REN3A antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human, Zebrafish, Recombinant fragment - Zebrafish samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR24112-81
Purification technique
Affinity purification Protein A
Specificity

This antibody cross-reacts with zebrafish Upf3b.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab288302 is the carrier free version of Anti-UPF3B/RENT3B + UPF3A antibody [EPR24112-81] ab269998

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

UPF3B also known as RENT3B is a component of the nonsense-mediated mRNA decay (NMD) pathway with a mass of approximately 48 kDa. UPF3A is a closely related homolog sharing several functional characteristics. UPF3B is expressed in various tissues including the brain. This protein primarily functions in the nucleus and cytoplasm where it binds to mRNA and interacts with other NMD factors to facilitate the degradation of faulty transcripts. By tagging mRNA with premature stop codons for degradation UPF3B helps maintain the integrity of gene expression.

Biological function summary

UPF3B ensures accuracy in post-transcriptional regulation. Both UPF3B and UPF3A are part of the exon-junction complex (EJC) playing a role in recognizing erroneous transcripts. They work in conjunction with other core NMD proteins such as UPF1 and UPF2 to target mRNAs with errors. This mechanism is essential in protecting cells from potential damage caused by erroneous or incomplete protein products impacting gene expression at the mRNA level.

Pathways

UPF3B and UPF3A play important roles in the nonsense-mediated mRNA decay pathway which is a critical mRNA surveillance mechanism. Their interaction with UPF1 an important factor in NMD illustrates their central position in this pathway. UPF3B's role in regulating mRNA stability also links it to the broader RNA processing and degradation pathways including those involved in general mRNA turnover and quality control ensuring cellular homeostasis.

Associated diseases and disorders

UPF3B associations relate to X-linked intellectual disabilities and neurodevelopmental disorders. Mutations in the UPF3B gene disrupt normal NMD function leading to cognitive impairments. This disruption often involves UPF1 and other NMD components. Such genetic defects highlight the importance of robust mRNA surveillance systems in neurological development and health. Furthermore the impaired function of UPF3B in this context reveals insights into the molecular basis of certain intellectual disabilities.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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