AB251022

Anti-UQCRB antibody [EPR15591] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal UQCRB antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

UQBP, UQCRB, Cytochrome b-c1 complex subunit 7, Complex III subunit 7, Complex III subunit VII, QP-C, Ubiquinol-cytochrome c reductase complex 14 kDa protein

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-UQCRB antibody [EPR15591] - BSA and Azide free (AB251022)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-UQCRB antibody [EPR15591] - BSA and Azide free (AB251022)

This data was developed using ab190360, the same antibody clone in a different buffer formulation.

Immunocytochemical analysis of HeLa cells fixed in 4% paraformaldehyde labeling UQCRB with ab190360 at 1/250 dilution and Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 dilution. Counterstained with DAPI. Negative controls also shown.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRB antibody [EPR15591] - BSA and Azide free (AB251022)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRB antibody [EPR15591] - BSA and Azide free (AB251022)

This data was developed using ab190360, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human adenocarcinoma of the colon labeling UQCRB with ab190360 at 1/100 and HRP Polymer for Rabbit IgG. Counterstained with hematoxylin. Negative control also shown.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-UQCRB antibody [EPR15591] - BSA and Azide free (AB251022)

This data was developed using ab190360, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-UQCRB antibody [EPR15591] (<a href='/en-us/products/primary-antibodies/uqcrb-antibody-epr15591-ab190360'>ab190360</a>) at 1/5000 dilution

All lanes:

293 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 14 kDa

Observed band size: 14 kDa

false

Supplier Data

Western blot - Anti-UQCRB antibody [EPR15591] - BSA and Azide free (AB251022)

This data was developed using ab190360, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-UQCRB antibody [EPR15591] (<a href='/en-us/products/primary-antibodies/uqcrb-antibody-epr15591-ab190360'>ab190360</a>) at 1/20000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

Human fetal heart tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 14 kDa

Observed band size: 14 kDa

false

Supplier Data

Western blot - Anti-UQCRB antibody [EPR15591] - BSA and Azide free (AB251022)

This data was developed using ab190360, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-UQCRB antibody [EPR15591] (<a href='/en-us/products/primary-antibodies/uqcrb-antibody-epr15591-ab190360'>ab190360</a>) at 1/1000 dilution

Lane 1:

Mouse heart tissue lysate at 10 µg

Lane 2:

Rat brain tissue lysate at 10 µg

Lane 3:

NIH/3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 14 kDa

false

Unconjugated

Anti-UQCRB antibody [EPR15591]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15591

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab251022 is the carrier-free version of ab190360.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UQCRB also known as ubiquinol-cytochrome c reductase binding protein is an important component of the mitochondrial electron transport chain specifically within the complex III (or cytochrome bc1 complex) of mitochondria. This protein has a molecular mass of approximately 14 kDa. It is localized in the inner membrane of mitochondria and is expressed in various tissues with high energy demands such as the heart liver and muscle tissues. UQCRB plays a significant mechanical role in transporting electrons from ubiquinol to cytochrome c a critical step in cellular respiration.

Biological function summary

The ubiquinol-cytochrome c reductase binding protein participates in facilitating efficient electron transfer within complex III. As a constituent of the cytochrome bc1 complex UQCRB contributes to the formation of a proton gradient across the mitochondrial membrane which is necessary for the production of ATP. This process is part of oxidative phosphorylation the principal pathway for energy generation in eukaryotic cells. UQCRB interacts closely with other components of the complex III such as cytochrome b and cytochrome c1 ensuring proper electron flow and energy transduction.

Pathways

UQCRB has a pronounced role in the oxidative phosphorylation pathway. This pathway is fundamental in the synthesis of ATP the energy currency in cells through the chemiosmotic coupling of electron transport and phosphorylation. UQCRB serves as a bridging protein for the coherent transfer of electrons from NADH:ubiquinone oxidoreductase (complex I) and succinate dehydrogenase (complex II) towards the cytochrome c oxidase (complex IV). It is intricately linked to other mitochondrial proteins like cytochrome c and ATP synthase that play roles in energy conversion and utilization.

UQCRB is associated with mitochondrial disorders specifically with mitochondrial complex III deficiency. This condition has been implicated in various pathologies including Leigh syndrome a severe neurological disorder. Mutations in the UQCRB gene can impair electron transport leading to insufficient ATP production and increased production of reactive oxygen species which contribute to cellular damage. UQCRB's association with other components of complex III such as cytochrome b may exacerbate these deficiencies and highlight its potential as a therapeutic target for mitochondrial dysfunction-related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. The cytochrome b-c1 complex catalyzes electron transfer from ubiquinol to cytochrome c, linking this redox reaction to translocation of protons across the mitochondrial inner membrane, with protons being carried across the membrane as hydrogens on the quinol. In the process called Q cycle, 2 protons are consumed from the matrix, 4 protons are released into the intermembrane space and 2 electrons are passed to cytochrome c.

See full target information UQCRB

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 21:454-462 PubMed31746378

2019

Blueberry anthocyanin‑enriched extract ameliorates transverse aortic constriction‑induced myocardial dysfunction via the DDAH1/ADMA/NO signaling pathway in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Weiqing Hu,Wenyue Wang,Qing Ma,Tao Liu,Jiefeng Zhang,Jicun Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-UQCRB antibody [EPR15591] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-UQCRB antibody [EPR15591] - BSA and Azide free (AB251022)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRB antibody [EPR15591] - BSA and Azide free (AB251022)

Western blot - Anti-UQCRB antibody [EPR15591] - BSA and Azide free (AB251022)

Western blot - Anti-UQCRB antibody [EPR15591] - BSA and Azide free (AB251022)

Western blot - Anti-UQCRB antibody [EPR15591] - BSA and Azide free (AB251022)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise