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Mouse Monoclonal UQCRC2 antibody. Suitable for IHC-P, Flow Cyt, WB, IF and reacts with Human samples. Cited in 293 publications.


Images

Western blot - Anti-UQCRC2 antibody [13G12AF12BB11] (AB14745), expandable thumbnail
  • Flow Cytometry - Anti-UQCRC2 antibody [13G12AF12BB11] (AB14745), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRC2 antibody [13G12AF12BB11] (AB14745), expandable thumbnail
  • Immunofluorescence - Anti-UQCRC2 antibody [13G12AF12BB11] (AB14745), expandable thumbnail
  • Western blot - Anti-UQCRC2 antibody [13G12AF12BB11] (AB14745), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.4 - 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline

Form
Liquid
Clonality
Monoclonal

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PFlow CytWBIF
Human
Tested
Tested
Tested
Tested
Pig
Predicted
Predicted
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1 µg/mL
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Pig
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1 µg for 106 Cells
Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Predicted
Predicted

Species
Pig
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1 µg/mL
Notes

-

Predicted
Predicted

Species
Pig
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
0.10000-0.50000 µg/mL
Notes

Requires heat-induced antigen retrieval where aldehydes are used as fixatives.

Predicted
Predicted

Species
Pig
Dilution info
-
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. The cytochrome b-c1 complex catalyzes electron transfer from ubiquinol to cytochrome c, linking this redox reaction to translocation of protons across the mitochondrial inner membrane, with protons being carried across the membrane as hydrogens on the quinol. In the process called Q cycle, 2 protons are consumed from the matrix, 4 protons are released into the intermembrane space and 2 electrons are passed to cytochrome c (By similarity). The 2 core subunits UQCRC1/QCR1 and UQCRC2/QCR2 are homologous to the 2 mitochondrial-processing peptidase (MPP) subunits beta-MPP and alpha-MPP respectively, and they seem to have preserved their MPP processing properties (By similarity). May be involved in the in situ processing of UQCRFS1 into the mature Rieske protein and its mitochondrial targeting sequence (MTS)/subunit 9 when incorporated into complex III (Probable).

Alternative names

Complex III subunit 2, Core protein II, Ubiquinol-cytochrome-c reductase complex core protein 2, UQCRC2

Recommended products

Mouse Monoclonal UQCRC2 antibody. Suitable for IHC-P, Flow Cyt, WB, IF and reacts with Human samples. Cited in 293 publications.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Clone number
13G12AF12BB11
Purity
IgG fraction
Light chain type
kappa
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

UQCRC2 also known as QCR2 is a subunit of the mitochondrial complex III also referred to as cytochrome bc1 complex. It has a molecular weight of approximately 48 kDa. This protein is located in the inner mitochondrial membrane across various tissue types playing an essential role in the mitochondrial electron transport chain. UQCRC2 facilitates the transfer of electrons from ubiquinol to cytochrome c which is an important step in cellular energy production.

Biological function summary

UQCRC2 participates in producing ATP which is the primary energy currency in cells. It forms part of the mitochondrial complex III a multi-subunit enzyme complex essential for efficient electron transfer and proton pumping. UQCRC2 contributes to establishing the proton gradient across the mitochondrial membrane which drives ATP synthesis through chemiosmotic processes. This function is critical for maintaining cellular energy homeostasis.

Pathways

The functionality of UQCRC2 links significantly to the oxidative phosphorylation and respiratory chain pathways. It ensures proper function in the oxidative phosphorylation pathway which generates the majority of ATP in aerobic organisms. UQCRC2 is associated with proteins such as cytochrome b and cytochrome c1 which collaborate in the electron transport chain ensuring the efficiency of the process and integrity of energy conversion.

Associated diseases and disorders

The aberrant function of UQCRC2 associates with mitochondrial disorders and certain cancers. Mutations affecting UQCRC2 function can lead to mitochondrial diseases characterized by energy production deficits resulting in neuromuscular and metabolic complications. Additionally studies have linked altered UQCRC2 expression to cancer where it may interact with proteins like p53 playing roles in metabolic reprogramming of cells.

Product promise

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5 product images

  • Western blot - Anti-UQCRC2 antibody [13G12AF12BB11] (ab14745), expandable thumbnail
    This image is courtesy of an anonymous customer review

    Western blot - Anti-UQCRC2 antibody [13G12AF12BB11] (ab14745)

    All lanes: Western blot - Anti-UQCRC2 antibody [13G12AF12BB11] (ab14745) at 1/1000 dilution

    All lanes: H23 whole cell lysate at 20 µg

    Secondary

    All lanes: HRP-conjugated goat anti-mouse IgG polyclonal at 1/2000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 48 kDa

    Observed band size: 48 kDa

    Exposure time: 1min

  • Flow Cytometry - Anti-UQCRC2 antibody [13G12AF12BB11] (ab14745), expandable thumbnail

    Flow Cytometry - Anti-UQCRC2 antibody [13G12AF12BB11] (ab14745)

    Overlay histogram showing HepG2 cells stained with ab14745 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14745, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRC2 antibody [13G12AF12BB11] (ab14745), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRC2 antibody [13G12AF12BB11] (ab14745)

    IHC image of ab14745 staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab14745, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunofluorescence - Anti-UQCRC2 antibody [13G12AF12BB11] (ab14745), expandable thumbnail

    Immunofluorescence - Anti-UQCRC2 antibody [13G12AF12BB11] (ab14745)

    Mitochondrial localization of complex III visualized by immunofluorescence using anti-complex III subunit Core 2 mAb 2E3GC12FB2AE2 (ab14745). Cultured human embryonic lung-derived fibroblasts (strain MRC5) were fixed, permeabilized and then labeled with ab14745 (0.5 μg/ml) followed by an AlexaFluor® 488-conjugated-goat-anti-mouse IgG1 isotype specific secondary antibody (2 μg/ml).

  • Western blot - Anti-UQCRC2 antibody [13G12AF12BB11] (ab14745), expandable thumbnail

    Western blot - Anti-UQCRC2 antibody [13G12AF12BB11] (ab14745)

    All lanes: Western blot - Anti-UQCRC2 antibody [13G12AF12BB11] (ab14745) at 0.5 µg/mL

    Lane 1: Human skeletal muscle tissue lysate at 10 µg

    Lane 2: Ramos (human Burkitt's lymphoma cell line) whole cell lysate at 10 µg

    Secondary

    All lanes: Goat polyclonal to mouse IgG - H&L - pre-adsorbed (HRP) at 1/3000 dilution

    Predicted band size: 48 kDa

    Observed band size: 30 kDa, 48 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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