Anti-UQCRC2 antibody [EPR13051]

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-UQCRC2 antibody [EPR13051] (AB203832)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma) cells labeling UQCRC2 with ab203832 at 1/300 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HepG2 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

The negative controls are as follows : -
-ve control 1 : ab203832 at 1/300 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRC2 antibody [EPR13051] (AB203832)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling UQCRC2 with ab203832 at 1/150 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on Human liver tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-UQCRC2 antibody [EPR13051] (AB203832)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling UQCRC2 with ab203832 at 1/300 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on Jurkat cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

The negative controls are as follows : -
-ve control 1 : ab203832 at 1/300 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-UQCRC2 antibody [EPR13051] (AB203832)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling UQCRC2 with ab203832 at 1/150 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

• IP

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Immunoprecipitation - Anti-UQCRC2 antibody [EPR13051] (AB203832)

UQCRC2 was immunoprecipitated from 1mg of HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate with ab203832 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab203832 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.

Lane 1 : HEK-293 whole cell lysate 10ug (Input). Lane 2 : ab203832 IP in HEK-293 whole cell lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab203832 in HEK-293 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 1 second.

All lanes:

Immunoprecipitation - Anti-UQCRC2 antibody [EPR13051] (ab203832)

Predicted band size: 48 kDa

false

• IP

Supplier Data

Immunoprecipitation - Anti-UQCRC2 antibody [EPR13051] (AB203832)

UQCRC2 was immunoprecipitated from 1mg of HepG2 (Human liver hepatocellular carcinoma) whole cell lysate with ab203832 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab203832 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.
Lane 1 : HepG2 whole cell lysate 10ug (Input). Lane 2 : ab203832 IP in HepG2 whole cell lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab203832 in HepG2 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 1 second.

All lanes:

Immunoprecipitation - Anti-UQCRC2 antibody [EPR13051] (ab203832)

Predicted band size: 48 kDa

false

• WB

Supplier Data

Western blot - Anti-UQCRC2 antibody [EPR13051] (AB203832)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-UQCRC2 antibody [EPR13051] (ab203832) at 1/2000 dilution

Lane 1:

Human fetal heart lysate at 10 µg

Lane 2:

Human fetal kidney lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 48 kDa

Observed band size: 48 kDa

false

Exposure time: 3s

• WB

Supplier Data

Western blot - Anti-UQCRC2 antibody [EPR13051] (AB203832)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-UQCRC2 antibody [EPR13051] (ab203832) at 1/2000 dilution

Lane 1:

Human fetal brain lysate at 20 µg

Lane 2:

HepG2 (Human liver hepatocellular carcinoma) cell lysate at 20 µg

Lane 3:

HEK-293 (Human epithelial cells from embryonic kidney) cell lysate at 20 µg

Lane 4:

Jurkat (Human T cell leukemia cells from peripheral blood) cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 48 kDa

Observed band size: 48 kDa

false

Exposure time: 3s

• WB

Unknown

Western blot - Anti-UQCRC2 antibody [EPR13051] (AB203832)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-UQCRC2 antibody [EPR13051] (ab203832) at 1/2000 dilution

Lane 1:

Mouse heart lysate at 10 µg

Lane 2:

Rat brain lysate at 10 µg

Lane 3:

Rat kidney lysate at 10 µg

Lane 4:

C6 (Rat glial tumor cells) cell lysate at 10 µg

Lane 5:

PC-12 (Rat adrenal gland pheochromocytoma) cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 48 kDa

Observed band size: 48 kDa

false

Exposure time: 5s