Mouse Monoclonal UQCRFS1/RISP antibody. Suitable for Flow Cyt, WB, IHC-P and reacts with Human, Mouse, Cow, Rat samples. Cited in 80 publications.
Images
![Western blot - Anti-UQCRFS1/RISP antibody [5A5] (AB14746), expandable thumbnail](https://content.abcam.com/products/images/uqcrfs1-risp-antibody-5a5-ab14746--western-blot-img53126.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-UQCRFS1/RISP antibody [5A5] (AB14746)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRFS1/RISP antibody [5A5] (AB14746), expandable thumbnail](https://content.abcam.com/products/images/uqcrfs1-risp-antibody-5a5-ab14746--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img41546.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRFS1/RISP antibody [5A5] (AB14746)")
![Flow Cytometry - Anti-UQCRFS1/RISP antibody [5A5] (AB14746), expandable thumbnail](https://content.abcam.com/products/images/uqcrfs1-risp-antibody-5a5-ab14746--flow-cytometry-img80626.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-UQCRFS1/RISP antibody [5A5] (AB14746)")
![Western blot - Anti-UQCRFS1/RISP antibody [5A5] (AB14746), expandable thumbnail](https://content.abcam.com/products/images/uqcrfs1-risp-antibody-5a5-ab14746--western-blot-img99461.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-UQCRFS1/RISP antibody [5A5] (AB14746)")
Publications
Filter by
- Nature 625:385-3922023Mitochondrial dysfunction abrogates dietary lipid processing in enterocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChrysanthi Moschandrea,Vangelis Kondylis,Ioannis Evangelakos,Marija Herholz,Farina Schneider,Christina Schmidt,Ming Yang,Sandra Ehret,Markus Heine,Michelle Y Jaeckstein,Karolina Szczepanowska,Robin Schwarzer,Linda Baumann,Theresa Bock,Efterpi Nikitopoulou,Susanne Brodesser,Marcus Krüger,Christian Frezza,Joerg Heeren,Aleksandra Trifunovic,Manolis PasparakisPubMed 38123683
- Basic research in cardiology 118:152023Sexual dimorphism in bidirectional SR-mitochondria crosstalk in ventricular cardiomyocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRichard T Clements,Radmila Terentyeva,Shanna Hamilton,Paul M L Janssen,Karim Roder,Benjamin Y Martin,Fruzsina Perger,Timothy Schneider,Zuzana Nichtova,Anindhya S Das,Roland Veress,Beth S Lee,Do-Gyoon Kim,Gideon Koren,Matthew S Stratton,Gyorgy Csordas,Federica Accornero,Andriy E Belevych,Sandor Gyorke,Dmitry TerentyevPubMed 37138037
- Nature communications 14:3122023A FRET-based respirasome assembly screen identifies spleen tyrosine kinase as a target to improve muscle mitochondrial respiration and exercise performance in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAmi Kobayashi,Kotaro Azuma,Toshihiko Takeiwa,Toshimori Kitami,Kuniko Horie,Kazuhiro Ikeda,Satoshi InouePubMed 36697396
- Cancer discovery 12:2180-21972022NCOA4-Mediated Ferritinophagy Is a Pancreatic Cancer Dependency via Maintenance of Iron Bioavailability for Iron-Sulfur Cluster Proteins.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNaiara Santana-Codina,Maria Quiles Del Rey,Kevin S Kapner,Huan Zhang,Ajami Gikandi,Callum Malcolm,Clara Poupault,Miljan Kuljanin,Kristen M John,Douglas E Biancur,Brandon Chen,Nupur K Das,Kristen E Lowder,Connor J Hennessey,Wesley Huang,Annan Yang,Yatrik M Shah,Jonathan A Nowak,Andrew J Aguirre,Joseph D ManciasPubMed 35771492
- Frontiers in cell and developmental biology 10:7862682022Applying Sodium Carbonate Extraction Mass Spectrometry to Investigate Defects in the Mitochondrial Respiratory Chain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDavid R L Robinson,Daniella H Hock,Linden Muellner-Wong,Roopasingam Kugapreethan,Boris Reljic,Elliot E Surgenor,Carlos H M Rodrigues,Nikeisha J Caruana,David A StroudPubMed 35300415
- Nucleic acids research 49:13108-131212021FARS2 deficiency in Drosophila reveals the developmental delay and seizure manifested by aberrant mitochondrial tRNA metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenlu Fan,Xiaoye Jin,Man Xu,Yongmei Xi,Weiguo Lu,Xiaohang Yang,Min-Xin Guan,Wanzhong GePubMed 34878141
- International journal of molecular sciences 22:2021γ-Tocotrienol Protects against Mitochondrial Dysfunction, Energy Deficits, Morphological Damage, and Decreases in Renal Functions after Renal Ischemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGrazyna Nowak,Judit MegyesiPubMed 34884479
- Cell reports 35:1090022021NDUFS3 depletion permits complex I maturation and reveals TMEM126A/OPA7 as an assembly factor binding the ND4-module intermediate.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLuigi D'Angelo,Elisa Astro,Monica De Luise,Ivana Kurelac,Nikkitha Umesh-Ganesh,Shujing Ding,Ian M Fearnley,Giuseppe Gasparre,Massimo Zeviani,Anna Maria Porcelli,Erika Fernandez-Vizarra,Luisa IommariniPubMed 33882309
- Nature communications 12:21302021Coding and non-coding roles of MOCCI (C15ORF48) coordinate to regulate host inflammation and immunity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCheryl Q E Lee,Baptiste Kerouanton,Sonia Chothani,Shan Zhang,Ying Chen,Chinmay Kumar Mantri,Daniella Helena Hock,Radiance Lim,Rhea Nadkarni,Vinh Thang Huynh,Daryl Lim,Wei Leong Chew,Franklin L Zhong,David Arthur Stroud,Sebastian Schafer,Vinay Tergaonkar,Ashley L St John,Owen J L Rackham,Lena HoPubMed 33837217
- Cell reports 34:1088692021The mitochondrial carrier SFXN1 is critical for complex III integrity and cellular metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichelle Grace Acoba,Ebru S Selen Alpergin,Santosh Renuse,Lucía Fernández-Del-Río,Ya-Wen Lu,Oleh Khalimonchuk,Catherine F Clarke,Akhilesh Pandey,Michael J Wolfgang,Steven M ClaypoolPubMed 33730581
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
| Rat | Predicted | Expected | Expected |
| Cow | Expected | Expected | Expected |
| Pig | Predicted | Predicted | Predicted |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg for 106 Cells | Notes ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg for 106 Cells | Notes ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Species Cow | Dilution info 1 µg for 106 Cells | Notes ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Pig | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
Species Mouse | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1 µg/mL | Notes - |
Species Cow | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 2 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 2 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Cow | Dilution info 2 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Cytochrome b-c1 complex subunit Rieske, mitochondrial. Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. The cytochrome b-c1 complex catalyzes electron transfer from ubiquinol to cytochrome c, linking this redox reaction to translocation of protons across the mitochondrial inner membrane, with protons being carried across the membrane as hydrogens on the quinol. In the process called Q cycle, 2 protons are consumed from the matrix, 4 protons are released into the intermembrane space and 2 electrons are passed to cytochrome c. The Rieske protein is a catalytic core subunit containing a [2Fe-2S] iron-sulfur cluster. It cycles between 2 conformational states during catalysis to transfer electrons from the quinol bound in the Q(0) site in cytochrome b to cytochrome c1 (By similarity). Incorporation of UQCRFS1 is the penultimate step in complex III assembly (By similarity). Cytochrome b-c1 complex subunit 9. Component of the ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII). UQCRFS1 undergoes proteolytic processing once it is incorporated in the complex III dimer. One of the fragments, called subunit 9, corresponds to its mitochondrial targeting sequence (MTS) (PubMed:2996928, PubMed:8386158). The proteolytic processing is necessary for the correct insertion of UQCRFS1 in the complex III dimer, but the persistence of UQCRFS1-derived fragments may prevent newly imported UQCRFS1 to be processed and assembled into complex III and is detrimental for the complex III structure and function (By similarity).
Alternative names
Complex III subunit 5, Cytochrome b-c1 complex subunit 5, Rieske iron-sulfur protein, Rieske protein UQCRFS1, Ubiquinol-cytochrome c reductase iron-sulfur subunit, RISP, UQCRFS1
Recommended products
Mouse Monoclonal UQCRFS1/RISP antibody. Suitable for Flow Cyt, WB, IHC-P and reacts with Human, Mouse, Cow, Rat samples. Cited in 80 publications.
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- 5A5
- Purification technique
- Affinity purification Immunogen
- Light chain type
- kappa
- Concentration
- Purification notes
Near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Product was previously marketed under the MitoSciences sub-brand.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
UQCRFS1 also known as RISP functions as an essential component of the mitochondrial electron transport chain. The protein weighs approximately 29 kDa and plays an important role in the transfer of electrons from ubiquinol to cytochrome c within complex III also named cytochrome bc1 complex. UQCRFS1 expresses primarily in tissues with high energy requirements such as heart and skeletal muscle due to their reliance on aerobic metabolism for ATP production.
- Biological function summary
RISP belongs to the cytochrome bc1 complex a large multimeric structure involved in mitochondrial respiratory function. By facilitating electron transfer it contributes to the establishment of the proton gradient across the inner mitochondrial membrane. This gradient drives ATP synthesis through oxidative phosphorylation highlighting its importance for cellular energy homeostasis. The reduction and oxidation of ubiquinone and cytochrome c ensure effective electron flow within this system.
- Pathways
RISP integrates into essential processes within the respiratory chain and oxidative phosphorylation pathways. It directly interacts with other proteins in complex III such as UQCRH and UQCRC1 to facilitate protein complex stability and function. Additionally RISP supports the interaction between complex III and complex IV by aiding in the electron transfer ensuring efficient ATP production. This association clarifies the interprotein dynamics needed for proper mitochondrial respiratory operation.
- Associated diseases and disorders
RISP connects to mitochondrial dysfunction and related pathologies. Mitochondrial complex III deficiencies often link to neurodegenerative disorders like Leigh syndrome due to defective oxidative phosphorylation. Such deficiencies may result from perturbations in RISP function or expression affecting electron transport chain efficiency. Furthermore disruptions in RISP activity relate to ischemic conditions where hypoxic stress compromises ATP production implicating it together with cytochrome c in cellular energy crises.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-UQCRFS1/RISP antibody [5A5] (ab14746), expandable thumbnail](https://content.abcam.com/products/images/uqcrfs1-risp-antibody-5a5-ab14746--western-blot-img53126.jpg "Western blot - Anti-UQCRFS1/RISP antibody [5A5] (ab14746)")
Western blot - Anti-UQCRFS1/RISP antibody [5A5] (ab14746)
All lanes: Western blot - Anti-UQCRFS1/RISP antibody [5A5] (ab14746) at 0.5 µg/mL
Lane 1: Hela Whole Cell Lysate - Hydroxyurea Treated at 10 µg
Lane 2: Hela Whole Cell Lysate - Bleomycin Treated at 10 µg
Lane 3: Hela Whole Cell Lysate - Staurosporine Treated at 10 µg
SecondaryAll lanes: Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 30 kDa
Observed band size: 25 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRFS1/RISP antibody [5A5] (ab14746), expandable thumbnail](https://content.abcam.com/products/images/uqcrfs1-risp-antibody-5a5-ab14746--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img41546.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRFS1/RISP antibody [5A5] (ab14746)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRFS1/RISP antibody [5A5] (ab14746)
Human normal kidney (cortex). Staining is observed intracellular. Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control. Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for mouse for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
![Flow Cytometry - Anti-UQCRFS1/RISP antibody [5A5] (ab14746), expandable thumbnail](https://content.abcam.com/products/images/uqcrfs1-risp-antibody-5a5-ab14746--flow-cytometry-img80626.jpg "Flow Cytometry - Anti-UQCRFS1/RISP antibody [5A5] (ab14746)")
Flow Cytometry - Anti-UQCRFS1/RISP antibody [5A5] (ab14746)
Overlay histogram showing Raji cells stained with ab14746 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14746, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (Mouse IgG2b [PLPV219] - Isotype Control ab91366, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Raji cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
![Western blot - Anti-UQCRFS1/RISP antibody [5A5] (ab14746), expandable thumbnail](https://content.abcam.com/products/images/uqcrfs1-risp-antibody-5a5-ab14746--western-blot-img99461.jpg "Western blot - Anti-UQCRFS1/RISP antibody [5A5] (ab14746)")
Western blot - Anti-UQCRFS1/RISP antibody [5A5] (ab14746)
All lanes: Western blot - Anti-UQCRFS1/RISP antibody [5A5] (ab14746) at 1 µg/mL
Lane 1: Isolated mitochondria from human heart at 5 µg
Lane 2: Isolated mitochondria from bovine heart at 1 µg
Lane 3: Isolated mitochondria from rat heart at 10 µg
Lane 4: Isolated mitochondria from mouse heart at 10 µg
Predicted band size: 30 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com