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AB251053

Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal UQCRFS1/RISP antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

Complex III subunit 5, Cytochrome b-c1 complex subunit 5, Rieske iron-sulfur protein, Rieske protein UQCRFS1, Ubiquinol-cytochrome c reductase iron-sulfur subunit, RISP, UQCRFS1

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free (AB251053)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free (AB251053)

This data was developed using ab191078, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling UQCRFS1/RISP with ab191078 at 1/500. Secondary antibody Goat anti rabbit IgG (HRP) prediluted. Counter stain Hematoxylin. Negative control also shown.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free (AB251053)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free (AB251053)

This data was developed using ab191078, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human ovarian adenocarcinoma tissue labeling UQCRFS1/RISP with ab191078 at 1/500. Secondary antibody Goat anti rabbit IgG (HRP) prediluted. Counter stain Hematoxylin. Negative control also shown.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free (AB251053)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free (AB251053)

This data was developed using ab191078, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of methanol-fixed HeLa cells labeling UQCRFS1/RISP with ab191078 at 1/50, Goat anti rabbit IgG (Alexa Fluor® 488) at 1/200 and DAPI staining (blue). Negative controls also shown.

Immunoprecipitation - Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free (AB251053)
  • IP

Supplier Data

Immunoprecipitation - Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free (AB251053)

This data was developed using ab191078, the same antibody clone in a different buffer formulation.

Western blot analysis of UQCRFS1/RISP in immunoprecipitation pellets from Human fetal lysate. ab191078 used at 1/60 dilution (Lane 1) or PBS (Lane 2). Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-UQCRFS1/RISP antibody [EPR16288] (<a href='/en-us/products/primary-antibodies/uqcrfs1-risp-antibody-epr16288-ab191078'>ab191078</a>)

Predicted band size: 30 kDa

Observed band size: 25 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free (AB251053)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free (AB251053)

This data was developed using ab191078, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling UQCRFS1/RISP with ab191078 at 1/500. Secondary antibody Goat anti rabbit IgG (HRP) prediluted. Counter stain Hematoxylin. Negative control also shown.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Western blot - Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free (AB251053)
  • WB

Supplier Data

Western blot - Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free (AB251053)

This data was developed using ab191078, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-UQCRFS1/RISP antibody [EPR16288] (<a href='/en-us/products/primary-antibodies/uqcrfs1-risp-antibody-epr16288-ab191078'>ab191078</a>) at 1/10000 dilution

Lane 1:

HeLa lysate at 20 µg

Lane 2:

Raji lysates at 20 µg

Lane 3:

293 lysates at 20 µg

Lane 4:

Human fetal heart lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 30 kDa

Observed band size: 25 kDa

false

Western blot - Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free (AB251053)
  • WB

Supplier Data

Western blot - Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free (AB251053)

This data was developed using ab191078, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-UQCRFS1/RISP antibody [EPR16288] (<a href='/en-us/products/primary-antibodies/uqcrfs1-risp-antibody-epr16288-ab191078'>ab191078</a>) at 1/1000 dilution

Lane 1:

mouse brain lysate at 10 µg

Lane 2:

mouse heart lysate at 10 µg

Lane 3:

mouse kidney lysate at 10 µg

Lane 4:

rat brain lysate at 10 µg

Lane 5:

rat heart lysate at 10 µg

Lane 6:

rat kidney lysate at 10 µg

Lane 7:

RAW264.7 lysate at 10 µg

Lane 8:

NIH3T3 lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 30 kDa

Observed band size: 25 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16288

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB, IHC-P, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Primary Antibodies

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Primary Antibodies

AB14715

Anti-SDHA antibody [2E3GC12FB2AE2]

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Western blot - Anti-SDHA antibody [2E3GC12FB2AE2] (AB14715)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab251053 is the carrier-free version of ab191078.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UQCRFS1 also known as RISP functions as an essential component of the mitochondrial electron transport chain. The protein weighs approximately 29 kDa and plays an important role in the transfer of electrons from ubiquinol to cytochrome c within complex III also named cytochrome bc1 complex. UQCRFS1 expresses primarily in tissues with high energy requirements such as heart and skeletal muscle due to their reliance on aerobic metabolism for ATP production.
Biological function summary

RISP belongs to the cytochrome bc1 complex a large multimeric structure involved in mitochondrial respiratory function. By facilitating electron transfer it contributes to the establishment of the proton gradient across the inner mitochondrial membrane. This gradient drives ATP synthesis through oxidative phosphorylation highlighting its importance for cellular energy homeostasis. The reduction and oxidation of ubiquinone and cytochrome c ensure effective electron flow within this system.

Pathways

RISP integrates into essential processes within the respiratory chain and oxidative phosphorylation pathways. It directly interacts with other proteins in complex III such as UQCRH and UQCRC1 to facilitate protein complex stability and function. Additionally RISP supports the interaction between complex III and complex IV by aiding in the electron transfer ensuring efficient ATP production. This association clarifies the interprotein dynamics needed for proper mitochondrial respiratory operation.

RISP connects to mitochondrial dysfunction and related pathologies. Mitochondrial complex III deficiencies often link to neurodegenerative disorders like Leigh syndrome due to defective oxidative phosphorylation. Such deficiencies may result from perturbations in RISP function or expression affecting electron transport chain efficiency. Furthermore disruptions in RISP activity relate to ischemic conditions where hypoxic stress compromises ATP production implicating it together with cytochrome c in cellular energy crises.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytochrome b-c1 complex subunit Rieske, mitochondrial. Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation (PubMed : 31883641). The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. The cytochrome b-c1 complex catalyzes electron transfer from ubiquinol to cytochrome c, linking this redox reaction to translocation of protons across the mitochondrial inner membrane, with protons being carried across the membrane as hydrogens on the quinol. In the process called Q cycle, 2 protons are consumed from the matrix, 4 protons are released into the intermembrane space and 2 electrons are passed to cytochrome c. The Rieske protein is a catalytic core subunit containing a [2Fe-2S] iron-sulfur cluster. It cycles between 2 conformational states during catalysis to transfer electrons from the quinol bound in the Q(0) site in cytochrome b to cytochrome c1 (By similarity). Incorporation of UQCRFS1 is the penultimate step in complex III assembly (PubMed : 28673544).. Cytochrome b-c1 complex subunit 9. Component of the ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII). UQCRFS1 undergoes proteolytic processing once it is incorporated in the complex III dimer. One of the fragments, called subunit 9, corresponds to its mitochondrial targeting sequence (MTS). The proteolytic processing is necessary for the correct insertion of UQCRFS1 in the complex III dimer, but the persistence of UQCRFS1-derived fragments may prevent newly imported UQCRFS1 to be processed and assembled into complex III and is detrimental for the complex III structure and function.
See full target information Cytochrome b-c1 complex subunit Rieske, mitochondrial
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 21:454-462 PubMed31746378

2019

Blueberry anthocyanin‑enriched extract ameliorates transverse aortic constriction‑induced myocardial dysfunction via the DDAH1/ADMA/NO signaling pathway in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Weiqing Hu,Wenyue Wang,Qing Ma,Tao Liu,Jiefeng Zhang,Jicun Zhang
View all publications
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Product promise

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