AB191079

Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal UQCRFS1/RISP antibody. C-terminal. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Rat, Mouse samples. Cited in 1 publication.

View Alternative Names

Complex III subunit 5, Cytochrome b-c1 complex subunit 5, Rieske iron-sulfur protein, Rieske protein UQCRFS1, Ubiquinol-cytochrome c reductase iron-sulfur subunit, RISP, UQCRFS1

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal (AB191079)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal (AB191079)

Immunofluorescent analysis of HepG2 cells (4% Paraformaldehyde-fixed; 0.1% tritonX-100 permeabilized) labeling UQCRFS1/RISP with ab191079 at 1/100 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue). Negative controls also shown.

Flow Cytometry (Intracellular) - Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal (AB191079)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal (AB191079)

Intracellular flow cytometric analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling UQCRFS1/RISP with ab191079 at 1/280 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

Supplier Data

Western blot - Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal (AB191079)

All lanes:

Western blot - Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal (ab191079) at 1/1000 dilution

Lane 1:

PC12 cell lysate at 10 µg

Lanes 2 - 3:

Mouse brain lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugate at 1/1000 dilution

Predicted band size: 30 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal (AB191079)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal (AB191079)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling UQCRFS1/RISP with ab191079 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin (inset : negative control).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal (AB191079)

Western blot analysis of immunoprecipitation pellet from Human fetal heart lysate immunoprecipitated using ab191079 at 1/90 dilution (lane

or PBS control (lane 2).
Secondary : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal (ab191079)

Predicted band size: 30 kDa

false

Supplier Data

Western blot - Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal (AB191079)

All lanes:

Western blot - Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal (ab191079) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

Raji cell lysate at 20 µg

Lane 3:

293 cell lysate at 20 µg

Lane 4:

Human fetal heart lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugate at 1/1000 dilution

Predicted band size: 30 kDa

false

Carrier free

Anti-UQCRFS1/RISP antibody [EPR16289] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16289

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

Flow Cyt (Intra), IP, WB, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/90", "IP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/280", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UQCRFS1 also known as RISP functions as an essential component of the mitochondrial electron transport chain. The protein weighs approximately 29 kDa and plays an important role in the transfer of electrons from ubiquinol to cytochrome c within complex III also named cytochrome bc1 complex. UQCRFS1 expresses primarily in tissues with high energy requirements such as heart and skeletal muscle due to their reliance on aerobic metabolism for ATP production.

Biological function summary

RISP belongs to the cytochrome bc1 complex a large multimeric structure involved in mitochondrial respiratory function. By facilitating electron transfer it contributes to the establishment of the proton gradient across the inner mitochondrial membrane. This gradient drives ATP synthesis through oxidative phosphorylation highlighting its importance for cellular energy homeostasis. The reduction and oxidation of ubiquinone and cytochrome c ensure effective electron flow within this system.

Pathways

RISP integrates into essential processes within the respiratory chain and oxidative phosphorylation pathways. It directly interacts with other proteins in complex III such as UQCRH and UQCRC1 to facilitate protein complex stability and function. Additionally RISP supports the interaction between complex III and complex IV by aiding in the electron transfer ensuring efficient ATP production. This association clarifies the interprotein dynamics needed for proper mitochondrial respiratory operation.

RISP connects to mitochondrial dysfunction and related pathologies. Mitochondrial complex III deficiencies often link to neurodegenerative disorders like Leigh syndrome due to defective oxidative phosphorylation. Such deficiencies may result from perturbations in RISP function or expression affecting electron transport chain efficiency. Furthermore disruptions in RISP activity relate to ischemic conditions where hypoxic stress compromises ATP production implicating it together with cytochrome c in cellular energy crises.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cytochrome b-c1 complex subunit Rieske, mitochondrial. Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation (PubMed : 31883641). The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. The cytochrome b-c1 complex catalyzes electron transfer from ubiquinol to cytochrome c, linking this redox reaction to translocation of protons across the mitochondrial inner membrane, with protons being carried across the membrane as hydrogens on the quinol. In the process called Q cycle, 2 protons are consumed from the matrix, 4 protons are released into the intermembrane space and 2 electrons are passed to cytochrome c. The Rieske protein is a catalytic core subunit containing a [2Fe-2S] iron-sulfur cluster. It cycles between 2 conformational states during catalysis to transfer electrons from the quinol bound in the Q(0) site in cytochrome b to cytochrome c1 (By similarity). Incorporation of UQCRFS1 is the penultimate step in complex III assembly (PubMed : 28673544).. Cytochrome b-c1 complex subunit 9. Component of the ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII). UQCRFS1 undergoes proteolytic processing once it is incorporated in the complex III dimer. One of the fragments, called subunit 9, corresponds to its mitochondrial targeting sequence (MTS). The proteolytic processing is necessary for the correct insertion of UQCRFS1 in the complex III dimer, but the persistence of UQCRFS1-derived fragments may prevent newly imported UQCRFS1 to be processed and assembled into complex III and is detrimental for the complex III structure and function.

See full target information Cytochrome b-c1 complex subunit Rieske, mitochondrial

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nutrition & diabetes 8:1 PubMed29330446

2018

Effect of puerarin in promoting fatty acid oxidation by increasing mitochondrial oxidative capacity and biogenesis in skeletal muscle in diabetic rats.

Applications

Unspecified application

Species

Unspecified reactive species

Xiu-Fang Chen,Lei Wang,Yong-Zheng Wu,Shi-Yu Song,Hai-Yan Min,Yan Yang,Xuan He,Qiao Liang,Long Yi,Yong Wang,Qian Gao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal

Immunocytochemistry/ Immunofluorescence - Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal (AB191079)

Flow Cytometry (Intracellular) - Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal (AB191079)

Western blot - Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal (AB191079)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal (AB191079)

Immunoprecipitation - Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal (AB191079)

Western blot - Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal (AB191079)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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