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Rabbit Recombinant Monoclonal UQCRH antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Rat samples. Cited in 2 publications.

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Images

Western blot - Anti-UQCRH antibody [EPR9038(B)] (AB154803), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] (AB154803), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] (AB154803), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-UQCRH antibody [EPR9038(B)] (AB154803), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] (AB154803), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Not recommended
Tested
Mouse
Predicted
Predicted
Not recommended
Predicted
Rat
Expected
Tested
Not recommended
Expected

Tested
Tested

Species
Human
Dilution info
1/50 - 1/100
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Rat
Dilution info
1/1000 - 1/10000
Notes

-

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Rat, Human, Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10 - 1/100
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. The cytochrome b-c1 complex catalyzes electron transfer from ubiquinol to cytochrome c, linking this redox reaction to translocation of protons across the mitochondrial inner membrane, with protons being carried across the membrane as hydrogens on the quinol. In the process called Q cycle, 2 protons are consumed from the matrix, 4 protons are released into the intermembrane space and 2 electrons are passed to cytochrome c.

Alternative names

Complex III subunit 6, Complex III subunit VIII, Cytochrome c1 non-heme 11 kDa protein, Mitochondrial hinge protein, Ubiquinol-cytochrome c reductase complex 11 kDa protein, UQCRH

Recommended products

Rabbit Recombinant Monoclonal UQCRH antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Rat samples. Cited in 2 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR9038(B)
Purification technique
Affinity purification Protein A
Specificity

The immunogen used for this product shares 100% homology with UQCRHL. Cross-reactivity with this protein has not been confirmed experimentally.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

UQCRH also known as ubiquinol-cytochrome c reductase hinge protein plays an important role in the electron transport chain. It forms a part of the core structure of cytochrome bc1 complex (Complex III) in mitochondria. UQCRH has a molecular mass of about 9 kDa and is expressed ubiquitously in human tissues. Its primary role is to assist in transferring electrons by connecting cytochrome c1 and cytochrome c which is essential for cellular respiration and energy production.

Biological function summary

The hinge protein acts as a critical component of the mitochondrial respiratory chain complex III. UQCRH ensures the efficient transfer of electrons within the complex which contributes to generating a proton gradient across the inner mitochondrial membrane. This proton gradient drives ATP synthesis. It is a vital component of the larger respiratory chain complex which is important for aerobic cellular energy metabolism and plays an important role in overall mitochondrial function.

Pathways

UQCRH is a significant player in the oxidative phosphorylation pathway which is central to the production of ATP. The protein closely interacts with components of Complex III and adjacent complexes of the electron transport chain such as NADH dehydrogenase (Complex I) and cytochrome c oxidase (Complex IV). These interactions help UQCRH integrate smoothly within the pathway ensuring the consistent flow of electrons and maintenance of ATP production.

Associated diseases and disorders

UQCRH mutations or dysfunctions link to mitochondrial disorders such as mitochondrial complex III deficiency. Dysfunction in this protein disrupts normal mitochondrial respiration leading to various symptoms including myopathy and encephalopathy. Additionally impaired UQCRH function can contribute to the development of neurodegenerative diseases. Studies have observed connections between abnormal UQCRH activity and proteins such as superoxide dismutase-1 (SOD1) in disease states indicating its possible role in oxidative stress-related pathologies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Western blot - Anti-UQCRH antibody [EPR9038(B)] (ab154803), expandable thumbnail

    Western blot - Anti-UQCRH antibody [EPR9038(B)] (ab154803)

    All lanes: Western blot - Anti-UQCRH antibody [EPR9038(B)] (ab154803) at 1/1000 dilution

    Lane 1: Fetal liver lysate at 10 µg

    Lane 2: PC12 lysate at 10 µg

    Lane 3: C6 lysate at 10 µg

    Lane 4: Fetal brain lysate at 10 µg

    Lane 5: Human spleen lysate at 10 µg

    Lane 6: Saos 2 lysate at 10 µg

    Predicted band size: 11 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] (ab154803), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] (ab154803)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling UQCRH with ab154803 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] (ab154803), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] (ab154803)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling UQCRH with ab154803 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-UQCRH antibody [EPR9038(B)] (ab154803), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-UQCRH antibody [EPR9038(B)] (ab154803)

    Intracellular flow cytometric analysis of permeabilized Saos 2 cells labeling UQCRH with ab154803 at 1/10 dilution (red) or a rabbit IgG (green- negative control).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] (ab154803), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] (ab154803)

    Immunohistochemical analysis of paraffin embedded Human normal brain tissue using ab154803 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] (ab154803), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] (ab154803)

    Immunohistochemical analysis of paraffin embedded Human normal kidney tissue using ab154803 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] (ab154803), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] (ab154803)

    Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue using ab154803 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] (ab154803), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] (ab154803)

    Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab154803 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-UQCRH antibody [EPR9038(B)] (ab154803), expandable thumbnail

    Image collected and cropped by CiteAb under a CC-BY license from the publication

    Western blot - Anti-UQCRH antibody [EPR9038(B)] (ab154803)

    UQCRH western blot using anti-UQCRH antibody [EPR9038(B)] ab154803. Publication image and figure legend from Meng, S., Zhan, S., et al., 2020, Proteome Sci, PubMed 31889914.


    ab154803 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab154803 please see the product overview.

    Validation of TMT-based proteomics data. (a) Two DEPs (UQCRH and HMGN1) identified in proteomics data were validated by western blot. Knockdown of ALKBH7 resulted in upregulation of UQCRH and HMGN1 expression, and the opposite pattern of expression was detected in ALKBH7 overexpression cell lines, which were consistent with our proteomics data. (b) The relative intensity of UQCRH and HMGN1 in western blot (normalized by corresponding β-actin expression)

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Product protocols

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